Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21 | Escherichia coli |
His-tagged protein expressed in Escherichia coli BL21 | Enterobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Enterobacter sp. | |
2-oxoglutarate | - |
Escherichia coli | |
aminooxyacetic acid | - |
Enterobacter sp. | |
gabaculine | - |
Enterobacter sp. | |
hydroxylamine | - |
Enterobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
trimethylpyruvate | pH 8.0, 37°C | Enterobacter sp. | |
0.09 | - |
trimethylpyruvate | pH 8.0, 37°C | Escherichia coli | |
0.42 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
0.63 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. | |
0.78 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Enterobacter sp. | |
0.97 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. | |
0.99 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
1.64 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Escherichia coli | |
7.38 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Escherichia coli | |
9.52 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Enterobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. | - |
- |
- |
Enterobacter sp. TL3 | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Escherichia coli |
immobilized metal ion affinity chromatography (Ni2+) | Enterobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate | - |
Escherichia coli | 3-hydroxyadamantylglycine + 2-oxoglutarate | - |
? | |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate | - |
Enterobacter sp. | 3-hydroxyadamantylglycine + 2-oxoglutarate | - |
? | |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate | - |
Enterobacter sp. TL3 | 3-hydroxyadamantylglycine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxobutyric acid + L-glutamate | - |
Escherichia coli | L-valine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxobutyric acid + L-glutamate | - |
Enterobacter sp. | L-valine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxobutyric acid + L-glutamate | - |
Enterobacter sp. TL3 | L-valine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxovaleric acid + L-glutamate | - |
Escherichia coli | L-isoleucine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxovaleric acid + L-glutamate | - |
Enterobacter sp. | L-isoleucine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxovaleric acid + L-glutamate | - |
Enterobacter sp. TL3 | L-isoleucine + 2-oxoglutarate | - |
? | |
4-methyl-2-oxovaleric acid + L-glutamate | - |
Escherichia coli | L-leucine + 2-oxoglutarate | - |
? | |
4-methyl-2-oxovaleric acid + L-glutamate | - |
Enterobacter sp. | L-leucine + 2-oxoglutarate | - |
? | |
4-methyl-2-oxovaleric acid + L-glutamate | - |
Enterobacter sp. TL3 | L-leucine + 2-oxoglutarate | - |
? | |
trimethylpyruvate + L-glutamate | - |
Escherichia coli | L-tert-leucine + 2-oxoglutarate | - |
? | |
trimethylpyruvate + L-glutamate | - |
Enterobacter sp. | L-tert-leucine + 2-oxoglutarate | - |
? | |
trimethylpyruvate + L-glutamate | - |
Enterobacter sp. TL3 | L-tert-leucine + 2-oxoglutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
branched chain aminotransferase | - |
Escherichia coli |
branched chain aminotransferase | - |
Enterobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
trimethylpyruvate | pH 8.0, 37°C | Escherichia coli | |
2.59 | - |
trimethylpyruvate | pH 8.0, 37°C | Enterobacter sp. | |
2.9 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Enterobacter sp. | |
4.11 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Escherichia coli | |
6.61 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
12.15 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. | |
12.45 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Enterobacter sp. | |
13.75 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
16.84 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Escherichia coli | |
17.32 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
7.5 | - |
- |
Enterobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 58% activity at pH 6.0, 78% activity at pH 9.0 | Escherichia coli |
6 | 9 | 58% activity at pH 6.0, 78% activity at pH 9.0 | Enterobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Enterobacter sp. | |
0.56 | - |
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid | pH 8.0, 37°C | Escherichia coli | |
10.27 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Escherichia coli | |
13.89 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
15.74 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Escherichia coli | |
15.96 | - |
3-methyl-2-oxobutyric acid | pH 8.0, 37°C | Enterobacter sp. | |
17.85 | - |
4-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. | |
19.29 | - |
3-methyl-2-oxovaleric acid | pH 8.0, 37°C | Enterobacter sp. | |
24.44 | - |
trimethylpyruvate | pH 8.0, 37°C | Escherichia coli | |
43.16 | - |
trimethylpyruvate | pH 8.0, 37°C | Enterobacter sp. |