Literature summary for 2.6.1.42 extracted from

Ruan, J.; Hu, J.; Yin, A.; Wu, W.; Cong, X.; Feng, X.; Li, S.
Structure of the branched-chain aminotransferase from Streptococcus mutans (2012), Acta Crystallogr. Sect. D, 68, 996-1002.

Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment)	Organism
GST-tagged protein expressed in Escherichia coli BL21(DE3)	Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion method with PEG 3350 as the primary precipitant	Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38140	-	monomer with an additional five-amino-acid tag (GPLGS)MALDI-TOF-TOF	Streptococcus mutans

Organism

Organism	UniProt	Comment	Textmining
Streptococcus mutans	Q8DTW7	-	-
Streptococcus mutans UA159	Q8DTW7	-	-

Purification (Commentary)

Purification (Comment)	Organism
GST-affinity chromatography, cleavage of the GST tag, anion exchange chromatography, gel filtration	Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.74	-	glycine, pH 8.0, 3°C	Streptococcus mutans
5.23	-	L-tryptophan, pH 8.0, 3°C	Streptococcus mutans
77.03	-	L-valine, pH 8.0, 3°C	Streptococcus mutans
86.84	-	L-leucine, pH 8.0, 3°C	Streptococcus mutans
104.6	-	L-isoleucine, pH 8.0, 3°C	Streptococcus mutans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycine + 2-oxoglutarate	-	Streptococcus mutans	L-glutamic acid + glyoxylate	-	?
glycine + 2-oxoglutarate	-	Streptococcus mutans UA159	L-glutamic acid + glyoxylate	-	?
L-isoleucine + 2-oxoglutarate	-	Streptococcus mutans	3-methyl-2-oxopentanoic acid + L-glutamate	-	r
L-isoleucine + 2-oxoglutarate	-	Streptococcus mutans UA159	3-methyl-2-oxopentanoic acid + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Streptococcus mutans	4-methyl-2-oxopentanoic acid + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Streptococcus mutans UA159	4-methyl-2-oxopentanoic acid + L-glutamate	-	r
L-tryptophan + 2-oxoglutarate	-	Streptococcus mutans	L-glutamic acid + 3-indole-2-oxopropanoate	-	?
L-tryptophan + 2-oxoglutarate	-	Streptococcus mutans UA159	L-glutamic acid + 3-indole-2-oxopropanoate	-	?
L-valine + 2-oxoglutarate	-	Streptococcus mutans	3-methyl-2-oxobutanoic acid + L-glutamate	-	r
L-valine + 2-oxoglutarate	-	Streptococcus mutans UA159	3-methyl-2-oxobutanoic acid + L-glutamate	-	r

Subunits

Subunits	Comment	Organism
homodimer	gel filtration	Streptococcus mutans

Synonyms

Synonyms	Comment	Organism
branched-chain aminotransferase	-	Streptococcus mutans

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Release 2023.1 (January 2023)