Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | in cell extracts, alanine activity is inhibited by 50% by about 2.5 mM 2-oxoglutarate, while the aspartate activity is unaffected at this concentration. The highly purified enzyme is also inhibited by 2-oxoglutarate, but the extent of inhibition is slightly more than that in extracts. Thus, alanine activity is completely inhibited by 10 mM 2-oxoglutarate, and 36% of the aspartate activity is inhibited | Methanococcus aeolicus | |
L-aspartate | inhibits tyrosine transamination | Methanococcus aeolicus | |
L-cysteine | 10 mM, 30% inactivation | Methanococcus aeolicus | |
L-histidine | inhibition is relieved by high concentrations of substrates | Methanococcus aeolicus | |
additional information | the activities with 2 mM aspartate or alanine are not inhibited by 10 mM isoleucine, phenylalanine, arginine, asparagine, glutamine, glycine, lysine, methionine, proline, serine, and threonine | Methanococcus aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
2-oxoglutarate | pH 7.5, 37°C | Methanococcus aeolicus | |
0.5 | - |
2-oxoglutarate | pH 7.5, 37°C | Methanococcus aeolicus | |
3.5 | - |
L-alanine | pH 7.5, 37°C | Methanococcus aeolicus | |
3.7 | - |
L-aspartate | pH 7.5, 37°C | Methanococcus aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
gel filtration | Methanococcus aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Methanococcus aeolicus | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Methanococcus aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
pH 7.5, 37°C, enzyme in crude extract | Methanococcus aeolicus |
25.3 | - |
pH 7.5, 37°C, partiall purified enzyme | Methanococcus aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | 0.7% of the activity with L-aspartate | Methanococcus aeolicus | pyruvate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Methanococcus aeolicus | oxaloacetate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | 0.07% of the activity with L-aspartate | Methanococcus aeolicus | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | 0.08% of the activity with aspartate | Methanococcus aeolicus | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | 0.08% of the activity with L-aspartate | Methanococcus aeolicus | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | 0.04% of the activity with aspartate | Methanococcus aeolicus | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | i.e. 4-hydroxyphenylpyruvate | ? | |
L-tyrosine + 2-oxoglutarate | 0.04% of the activity with L-aspartate | Methanococcus aeolicus | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate aminotransferase | - |
Methanococcus aeolicus |
AspAT | - |
Methanococcus aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanococcus aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanococcus aeolicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanococcus aeolicus | isoelectric focusing | - |
4.4 |