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Literature summary for 2.5.1.54 extracted from

  • Tzin, V.; Malitsky, S.; Ben Zvi, M.M.; Bedair, M.; Sumner, L.; Aharoni, A.; Galili, G.
    Expression of a bacterial feedback-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of the shikimate pathway in Arabidopsis elucidates potential metabolic bottlenecks between primary and secondary metabolism (2012), New Phytol., 194, 430-439.
    View publication on PubMed

Application

Application Comment Organism
agriculture expression of wild-type enzyme and phenylalanine-feedback insensitive mutant L175Q in Arabidopsis thaliana. Transgenic plants have comparable phenotypes and are fully fertile. The levels of shikimate, prephenate and Phe are higher in the different lines expressing the mutant enzyme than in the lines expressing the natural feedback-sensitive bacterial enzyme, and the control plants. Results imply that the bacterial enzyme is active in the transgenic plants and, similar to its operation in bacteria, the feedback insensitivity trait of the mutant enzyme is fundamental for enhancement of the flow of primary carbon metabolites via the shikimate pathway into the production of aromatic amino acids also in the plant Escherichia coli

Protein Variants

Protein Variants Comment Organism
L175Q phenylalanine-feedback-insensitive mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AB91
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General Information

General Information Comment Organism
physiological function expression of wild-type enzyme and phenylalanine-feedback insensitive mutant L175Q in Arabidopsis thaliana. Transgenic plants have comparable phenotypes and are fully fertile. The levels of shikimate, prephenate and Phe are higher in the different lines expressing the mutant enzyme than in the lines expressing the natural feedback-sensitive bacterial enzyme, and the control plants. Results imply that the bacterial enzyme is active in the transgenic plants and, similar to its operation in bacteria, the feedback insensitivity trait of the mutant enzyme is fundamental for enhancement of the flow of primary carbon metabolites via the shikimate pathway into the production of aromatic amino acids also in the plant Escherichia coli