Information on EC 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.54
-
RECOMMENDED NAME
GeneOntology No.
3-deoxy-7-phosphoheptulonate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-dehydroquinate biosynthesis I
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
chorismate metabolism
-
-
Metabolic pathways
-
-
Phenylalanine, tyrosine and tryptophan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-94-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain U-32; Trp-sensitive isozyme
-
-
Manually annotated by BRENDA team
strain U-32
-
-
Manually annotated by BRENDA team
wild type strain WV2 contains DS I, a leaky L-Phe-requiring auxotroph mutant strain GH141 grown under L-Phe limitation possesses additional DS II activity
-
-
Manually annotated by BRENDA team
2 isozymes: DS-Co and DS-Mn; cv. JL-24
-
-
Manually annotated by BRENDA team
strain B-6
-
-
Manually annotated by BRENDA team
strain B-6
-
-
Manually annotated by BRENDA team
strain 12/60/X, strain 33/X; strain3/2; strain H1; strain H20
-
-
Manually annotated by BRENDA team
Bacteria H1
strain H1
-
-
Manually annotated by BRENDA team
Bacteria H20
strain H20
-
-
Manually annotated by BRENDA team
Bacteria strain3/2
strain3/2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ATCC 17409
-
-
Manually annotated by BRENDA team
basonym Alcaligenes eutrophus; strain H16
-
-
Manually annotated by BRENDA team
strain H16
-
-
Manually annotated by BRENDA team
i.e. Comamonas terrigena; strain ATCC 11299a, two distinct regulatory isoenzymes: DAHP synthase-phe and DAHP synthase-tyr
-
-
Manually annotated by BRENDA team
strain HE 401
-
-
Manually annotated by BRENDA team
strain NST37 (NST, ATCC31882)
-
-
Manually annotated by BRENDA team
strain W3110
-
-
Manually annotated by BRENDA team
strain ATCC 17724
-
-
Manually annotated by BRENDA team
Neurospora crassa 74-OR23-1A
strain 74-OR23-1A
-
-
Manually annotated by BRENDA team
strain 74A
-
-
Manually annotated by BRENDA team
cytosolic isozyme
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
plastidic isozyme DS-Mn and cytosolic isozyme DS-Co
-
-
Manually annotated by BRENDA team
2 isoenzymes: tyrosine-sensitive DAHP synthase-tyr and tryptophane-sensitive DAHP synthase-trp
-
-
Manually annotated by BRENDA team
strain RH1326
-
-
Manually annotated by BRENDA team
tyrosine-sensitive isozyme
-
-
Manually annotated by BRENDA team
cytosolic isozyme Ds-Co
-
-
Manually annotated by BRENDA team
strain T 24
-
-
Manually annotated by BRENDA team
strain T 24
-
-
Manually annotated by BRENDA team
strain A3(2); Trp-sensitive isozyme
-
-
Manually annotated by BRENDA team
strain A3(2)
-
-
Manually annotated by BRENDA team
strain 7C
-
-
Manually annotated by BRENDA team
strain 7C
-
-
Manually annotated by BRENDA team
2 classes of enzymes: class AroAI and AroAII; strain ATCC 33436
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenol-3-fluoropyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
-
enzyme does not discriminate between (E) and (Z)-form of phosphoenol-3-fluoropyruvate
-
-
?
phosphoenolpyruvate + (3S)-2-deoxyerythrose 4-phosphate + H2O
(5S)-[5H]-3,5-dideoxy-D-arabinoheptulosonate 7-phosphate + phosphate
show the reaction diagram
-
-
-
-
ir
phosphoenolpyruvate + 2-deoxy-D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate
3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D,L-lyxose
2-dehydro-3-deoxy-D,L-galacto-octonate + phosphate
show the reaction diagram
-
D-lyxose or L-lyxose, at 1.8% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-arabinose
2-dehydro-3-deoxy-D-gluco-octonate + phosphate
show the reaction diagram
-
at 0.7% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose
2-dehydro-3-deoxy-D-arabino-heptonate + phosphate
show the reaction diagram
-
at 93% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-fructose 1,6-diphosphate
?
show the reaction diagram
-
same activity as with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-fructose 6-phosphate
?
show the reaction diagram
-
same activity as with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-glucose 6-phosphate
?
show the reaction diagram
phosphoenolpyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-threo-hexonate + phosphate
show the reaction diagram
-
at 176% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-ribose 5-phosphate
3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-ribose 5-phosphate + H2O
3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-ribulose 5-phosphate
?
show the reaction diagram
-
at 12% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-threose
2-dehydro-3-deoxy-D-xylo-heptonate + phosphate
show the reaction diagram
-
at 92% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-xylose
2-dehydro-3-deoxy-D-ido-octonate + phosphate
show the reaction diagram
-
at 1% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-xylulose 5-phosphate
?
show the reaction diagram
phosphoenolpyruvate + DL-glyceraldehyde 3-phosphate
pyruvate + phosphate
show the reaction diagram
phosphoenolpyruvate + erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + glycolaldehyde
(S)-2-oxo-4,5-dihydroxypentanoate + phosphate
show the reaction diagram
-
at 245% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + glyoxylate
S-4-hydroxy-2-oxo-1,5-pentanedioate + R-4-hydroxy-2-oxo-1,5-pentanedioate + phosphate
show the reaction diagram
-
at 205% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-erythrose
2-dehydro-3-deoxy-L-arabino-heptonate + phosphate
show the reaction diagram
-
at 70% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-glyceraldehyde
2-dehydro-3-deoxy-L-threo-hexonate + phosphate
show the reaction diagram
-
at 212% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-threose
2-dehydro-3-deoxy-L-xylo-heptonate + phosphate
show the reaction diagram
-
at 52% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-xylose
2-dehydro-3-deoxy-L-ido-octonate + phosphate
show the reaction diagram
-
at 1% of the activity with D-erythrose 4-phosphate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
additional information
?
-
-
structure comparison between 3-deoxy-7-phosphoheptulonate synthase and 3-deoxy-D-manno-octulosonate 8-phosphate synthase, EC 4.1.2.16, reveal that they share a common ancestor and adopt the same catalytic strategy
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
0.1 mM, up to 500% of initial activity
Cd2+
-
activates, Tyr-sensitive isozyme
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2S)-1,2-epoxypropylphosphonic acid
-
-
(2R)-2-(phosphonooxy)propanoic acid
-
mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate
(2S)-2-(phosphonooxy)propanoic acid
-
mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid
-
trifluorinated phosphonate
(E)-2-methyl-3-phosphonoacrylic acid
-
most potent of the tested inhibitors mimicking intermediates in the reaction, vinyl phosphonate 4
1,10-phenanthroline
-
activity is restored by Fe2+ or Zn2+
2,3-bisphosphoglycerate
-
-
2-(phosphonomethyl)prop-2-enoic acid
-
mimics substrate phosphoenolpyruvate, and should be inert due to alkene structure but lacking an electron-donor, acts as competitive inhibitor
2-Methyl-DL-Trp
-
0.02 mM, 30% inhibition
2-phosphoglycerate
-
competitive with respect to phosphoenolpyruvate
3,4-dihydroxycinnamate
-
isoenzyme DS-Co
3-deoxy-D-arabino-heptonic acid 7-phosphate
-
-
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
-
-
3-Methylphosphoenolpyruvate
-
-
3-Propylphosphoenolpyruvate
-
-
4-Methyl-DL-Trp
-
0.02 mM, 30% inhibition
5,5'-dithiobis(2-nitrobenzoate)
-
-
5-Fluoro-DL-Trp
-
0.02 mM, 56% inhibition
5-hydroxy-DL-Trp
-
0.02 mM, 10% inhibition
6-Methyl-DL-Trp
-
0.02 mM, 6% inhibition
7-Aza-DL-Trp
-
0.02 mM, 9% inhibition
7-Methyl-DL-Trp
-
0.02 mM, 14% inhibition
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate
alpha-Methylphenylalanine
-
-
beta-2-Thienyl-D,L-Ala
-
-
beta-phenylserine
-
-
beta-Thienylalanine
-
-
chorismate
CN-
-
Tyr-sensitive isozyme, strong inhibition, reactivation by divalent cations only to a small extent
D-erythrose 4-phosphate
D-fructose 1,6-diphosphate
-
-
D-sedoheptulose 1,7-diphosphate
-
-
D-sedoheptulose 7-phosphate
-
-
diethyl dicarbonate
-
Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G
dihydroxyphenylalanine
-
-
dipicolinic acid
DL-Dibromotryptophan
-
0.02 mM, 52% inhibition
DL-erythro-beta-Methyltryptophan
-
0.02 mM, 40% inhibition
DL-Homotryptophan
-
0.02 mM, 46% inhibition
fosmidomycin
-
uncompetitive inhibitor, maximum level of inhibition after 10 min incubation, extent of inhibition dependent on the type of the metal cofactor, competitive inhibitor with respect to phosphoenolpyruvate
Hg2+
-
complete inhibition at 1 mM
iodoacetamide
-
alkylation, abolishes the dependence on reducing agents
L-arogenate
-
isozyme DS-Mn, competitive against D-erythrose 4-phosphate, non-competitive against phosphoenolpyruvate
L-Phe
-
NCgl0950 DAHP synthase is sensitive to feedback inhibition with 29.1 to 38.8% inhibition at 2 mM, NCgl2098 DAHP synthase is insensitive
L-phenylalanine
L-Trp
-
NCgl0950 DAHP synthase is sensitive to feedback inhibition, NCgl2098 DAHP synthase is slightly sensitive to feedback inhibition with 10-15% decrease of activity at 5 mM L-Trp
L-tryptophan
-
1 mM, 3% residual activity
L-Tyr
-
NCgl0950 DAHP synthase is sensitive to feedback inhibition with 80% loss of activity at 0.03 mM, NCgl2098 DAHP synthase is insensitive, Tyr-linked inhibition is competitive to phophoenolpyruvate
L-tyrosine
m-Chlorophenylalanine
-
-
m-Fluorophenylalanine
-
-
m-hydroxyphenylalanine
-
-
Metal chelators
-
-
-
Mg2+
-
no inhibition of mutant C67L; wild-type and mutants
N-alpha-methyl-DL-Trp
-
0.02 mM, 60% inhibition
N-bromosuccinimide
-
-
N-ethylmaleimide
-
-
N-[Phosphomonomethyl]glycine
Na2SO4
-
slightly
o-Chlorophenylalanine
-
-
o-Fluorophenylalanine
-
-
o-Hydroxyphenylalanine
-
-
p-aminophenylalanine
-
-
p-chloromercuribenzoate
-
complete inhibition at 0.02 mM, reversible by cysteine
p-Fluorophenylalanine
-
-
p-hydroxymercuribenzoate
-
-
phenylalanine
phenylpyruvate
-
DAHP synthase-tyr
phosphate
phosphoenolpyruvate
pronounced substrate inhibition above 1 mM
prephenate
shikimate
-
1 mM, 3% residual activity
tetraammonium (((carboxymethyl)[(2S,3R,4S)-2,3,4-trihydroxy-5-(phosphonatooxy)pentyl]amino)methyl)phosphonate
-
IC50: 0.0066 mM
Tetranitromethane
-
-
Trinitrobenzene sulfonate
-
-
tyrosine
[(1E)-7-bromo-2-carboxyhept-1-en-1-yl]phosphonate
-
inhibitor based on vinyl phosphonate, designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
[2-carboxy-7-(phosphonatooxy)hept-1-en-1-yl]phosphonate
-
inhibitor based on vinyl phosphonate ratio Z:E enantiomer 1:1. Inhibitor is designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
[2-carboxy-7-(phosphonatooxy)hept-2-en-1-yl]phosphonate
-
inhibitor based on allyl phosphonate, ratio Z:E enantiomer 7:3. Inhibitor is designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activates
beta-mercaptoethanol
-
-
Chorismic acid
dithiothreitol
N-[Phosphomonomethyl]glycine
-
i.e. glyphosate; induces synthesis of enzyme
Phe
-
slightly activating, mutants S187Y, S187F, S187C
reduced thioredoxin
-
required for activity, can be partially substituted by dithiothreitol
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
(3S)-2-deoxyerythrose 4-phosphate
-
pH 6.8
0.006
2-deoxy-D-erythrose 4-phosphate
-
pH 6.8, 60C
2.5
2-deoxy-D-ribose 5-phosphate
-
pH 6.8, 60C
6.8
2-deoxyribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25C
0.03 - 2.7
D-arabinose 5-phosphate
2.5
D-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37C
0.0026 - 52.36
D-erythrose 4-phosphate
3.5
D-glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37C
1.58
D-ribose 5-phosphate
-
pH 6.8, 60C
8.4
D-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37C
3
DL-glyceraldehyde 3-phosphate
-
cytosolic isozyme DS-Co, pH 8.6, 37C
0.006
erythrose 4-phosphate
-
pH 7.5, 30C
8.6
glycolaldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37C
3.6
glyoxylate
-
cytosolic isozyme DS-Co, pH 8.6, 37C
5.1
L-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37C
3.3
L-Glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37C
13.9
L-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37C
0.003 - 21
phosphoenolpyruvate
6
ribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25C
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.233
(3S)-2-deoxyerythrose 4-phosphate
Escherichia coli
-
pH 6.8
0.03 - 122
D-erythrose 4-phosphate
0.0035 - 122
phosphoenolpyruvate
additional information
additional information
Corynebacterium glutamicum
-
mutants
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.069 - 60
D-erythrose 4-phosphate
0.22 - 84
phosphoenolpyruvate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.049
(2R)-2-(phosphonooxy)propanoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.67
(2S)-2-(phosphonooxy)propanoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.0088
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.0047
(E)-2-methyl-3-phosphonoacrylic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.27
2-(phosphonomethyl)prop-2-enoic acid
1.5
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
-
pH 7.5, 30C, cosubstrate erythrose 4-phosphate
1.35 - 2.25
chorismate
0.035
fosmidomycin
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.01 - 0.27
Phe
1.35 - 2.55
phenylpyruvate
22 - 54
phosphate
0.005 - 0.04
Trp
0.0009 - 0.023
Tyr
additional information
additional information
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
chorismate
Bacillus subtilis
-
IC50: 1 mM
0.1
prephenate
Bacillus subtilis
-
IC50: 0.1 mM
0.0066
tetraammonium (((carboxymethyl)[(2S,3R,4S)-2,3,4-trihydroxy-5-(phosphonatooxy)pentyl]amino)methyl)phosphonate
Escherichia coli
-
IC50: 0.0066 mM
0.0036
[(1E)-7-bromo-2-carboxyhept-1-en-1-yl]phosphonate
Escherichia coli
-
pH not specified in the publication, temperature not specified in the publication
0.0053
[2-carboxy-7-(phosphonatooxy)hept-1-en-1-yl]phosphonate
Escherichia coli
-
pH not specified in the publication, temperature not specified in the publication
0.154
[2-carboxy-7-(phosphonatooxy)hept-2-en-1-yl]phosphonate
Escherichia coli
-
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.005
-
partially purified isozyme synthase-trp
0.008
-
partially purified isoenzyme DS-Mn
0.026
-
partially purified enzyme
0.056
-
partially purified isozyme synthase-tyr
0.11
-
partially purified isozyme DS-Mn, with Mn2+
0.14
-
partially purified isozyme DS-Co, with Co2+
0.21
-
partially purified isozyme DS-Co
0.27
-
partially purified Tyr-sensitive isozyme
0.29
-
N-terminal deletion mutant, 37C, pH 6.4
0.4
-
partially purified enzyme
0.8
-
recombinant clone M1 in Escherichia coli strain BL21(DE3)
0.81
-
mutant W215A, 37C, pH 6.4
0.91
-
purified enzyme
0.92
-
mutant I10A, 37C, pH 6.4
1.3
-
partially purified enzyme
1.97
-
mutant N5K, 37C, pH 6.4
2.1
-
purified enzyme
2.3
-
purified cytosolic isozyme
2.37
-
mutant V221A, 37C, pH 6.4
2.4
-
partially purified native enzyme
2.7
-
wild-type, 37C, pH 6.4
2.81
-
mutant L179A, 37C, pH 6.4; mutant P150L, 37C, pH 6.4
3.07
-
mutant F144A, 37C, pH 6.4
3.11
-
recombinant clone M2 in Escherichia coli strain BL21(DE3)
3.25
-
mutant F209A, 37C, pH 6.4
3.6
-
mutant L175Q, 37C, pH 6.4
4.25
-
mutant L175A, 37C, pH 6.4
4.46
-
mutant L175D, 37C, pH 6.4
4.5
-
partially purified Phe-sensitive isozyme
7.1
-
purified enzyme
7.7
-
purified Trp-sensitive isozyme
7.8
-
purified enzyme
8.1
-
purified Trp-sensitive isozyme
8.24
-
purified recombinant Tyr-sensitive isozyme
8.33
-
purified Trp-sensitive isozyme
10.75
-
purified enzyme
11.02
-
purified Trp-sensitive isozyme
16.4
-
purified enzyme
18.8
-
partially purified cytosolic isozyme, + 10 mM Mg2+
22
-
purified recombinant Tyr-sensitive isozyme, with Cd2+
30 - 40
-
purified Tyr-sensitive isozyme, wild-type and mutant N8K
56
-
purified enzyme
67
-
purified enzyme
82.6
-
purified enzyme
139.3
-
recombinant wild-type
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
Tris-HCl buffer
6.5
-
wild-type and mutants
6.5 - 7
-
-
6.8 - 7.2
-
DAHP synthase-phe
7 - 7.5
-
recombinant Tyr-sensitive isozyme
7 - 7.4
-
Trp-sensitive isozyme
7.2
-
phosphate buffer, Trp-sensitive isozyme
7.2 - 8.2
-
-
7.2
-
DAHP synthase-trp
7.3
-
tryptophan-sensitive isozyme
7.5
-
assay at; mutant enzyme I181D
7.5 - 8
-
chloroplastic isoenzyme DS-Mn
7.6
-
assay at
8
-
above, Phe-sensitive iaozyme mutant C61G
8.2 - 8.5
-
native enzyme
9.2
-
cytosolic isoenzyme DS-Co
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 8.5
-
wild-type and mutants
5.5 - 8.5
-
pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity
5.7 - 8.5
-
pH 5.7: about 25% of maximal activity, pH 8.5: about 30% of maximal activity
6 - 8.5
-
pH 6.0: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
6 - 9
-
at pH 9.0 the activity is about 60% of the activity at pH 6.0, wid-type enzyme; pH 6.0: about 55% of maximal activity, pH 9.0: about 35% of maximal activity, mutant enzyme I181D
6 - 7.6
-
pH 6.0: about 30% of maximal activity, pH 7.6: about 60% of maximal activity
6 - 7
-
more than 90% of maximum activity
6.2 - 7.9
-
pH 6.2: about 35% of maximal activity, pH 7.9: about 55% of maximal activity
6.5 - 9
-
pH 6.5: about 35% of maximal activity, pH 9.0: about 60% of maximal activity
7
60C, 40% of maximum activity
8.5 - 9.5
-
more than 85% of maximum activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
wild-type and mutants
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
presence of amino-terminal extension characteristic of chloroplast transit peptides on DSH1 and DSH2 suggests that both proteins may be targeted to the chloroplast
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)