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Literature summary for 2.5.1.54 extracted from

  • Webby, C.J.; Patchett, M.L.; Parker, E.J.
    Characterization of a recombinant type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Helicobacter pylori (2005), Biochem. J., 390, 223-230.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
-70°C, presence of 1 mM dithiothreitol, stable Helicobacter pylori
4°C, presence of 1 mM dithiothreitol, stabel for at least 24 h Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
-
Helicobacter pylori
EDTA inactivation, reactivation by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori
additional information not inhibitory: phenylalanine, tyrosine, tryptophan, chorismate Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
phosphoenolpyruvate pH 7.5, 30°C Helicobacter pylori
0.006
-
D-erythrose 4-phosphate pH 7.5, 30°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
additional information reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
x * 51000, SDS-PAGE Helicobacter pylori
100000
-
gel fitlration Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Helicobacter pylori

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered sequential mechanism Helicobacter pylori

Renatured (Commentary)

Renatured (Comment) Organism
reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information reaction involves interaction of si face of phosphoenolpyruvate with re face of eryxthrose 4-phosphate Helicobacter pylori ?
-
?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
-
Helicobacter pylori 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Helicobacter pylori 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-ribose 5-phosphate + H2O
-
Helicobacter pylori 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer x * 51000, SDS-PAGE Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
phosphoenolpyruvate pH 7.5, 30°C Helicobacter pylori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27
-
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate pH 7.5, 30°C, cosubstrate phosphoenolpyruvate Helicobacter pylori
1.5
-
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate pH 7.5, 30°C, cosubstrate erythrose 4-phosphate Helicobacter pylori