Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not hysteretically activated by dithiols, e.g. DTT | Xanthomonas campestris |
Cloned (Comment) | Organism |
---|---|
2 clones M1 and M2, expression in Escherichia coli strain BL21(DE3) and strain AB3248, which is defective in all endogenous isozyme forms, functional complementation of the mutant | Xanthomonas campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chorismate | main allosteric feedback inhibitor and regulator for enzyme of class AroAII | Xanthomonas campestris | |
EDTA | strong, reversible by dialysis | Xanthomonas campestris | |
Trp | minor feedback inhibitor | Xanthomonas campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Xanthomonas campestris | - |
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
strain ATCC 33436 | - |
Xanthomonas campestris | - |
2 classes of enzymes: class AroAI and AroAII | - |
Purification (Comment) | Organism |
---|---|
partial, 19fold, recombinant from Escherichia coli | Xanthomonas campestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
recombinant clone M1 in Escherichia coli strain BL21(DE3) | Xanthomonas campestris |
2.4 | - |
partially purified native enzyme | Xanthomonas campestris |
3.11 | - |
recombinant clone M2 in Escherichia coli strain BL21(DE3) | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Xanthomonas campestris | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | 2 distinct homology classes: AroAI and AroAII, both being found in bacteria and higher plant chloroplasts, AroAII can be devided into 4 subgroups, phylogenetic evolution | Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | native enzyme | Xanthomonas campestris |