Cloned (Comment) | Organism |
---|---|
gene aroF, Tyr-sensitive isozyme, expression of wild-type and mutants in strain AB3257 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutant of Tr-sensitive isozyme, gene aroF, lacking the first 7 amino acid residues of the N-terminus, mutant is insensitive against inhibition by tyrosine | Escherichia coli |
N8K | similar activity and substrate affinities like the wild-type, but insensitive against inhibition by tyrosine, decreased thermostability | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tyr | wild-type isozyme, mutant N8K and N-terminal deletion mutant are not inhibited, N-terminus is structurally involved in the sensitivity for feedback inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
Tyr-sensitive isozyme, mutant N8K, gel filtration | Escherichia coli |
75000 | - |
Tyr-sensitive isozyme, wild-type, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
K12 | - |
Escherichia coli | - |
tyrosine-sensitive isozyme | - |
Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Tyr-sensitive isozyme, wild-type and mutants from strain AB3257 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | 40 | purified Tyr-sensitive isozyme, wild-type and mutant N8K | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli W3110 / ATCC 27325 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
Tyr-sensitive isozyme, wild-type: 50% loss of activity in 80 min in presence of phosphoenolpyruvate, mutant N8K: 50% loss of activity in 20 min in presence of phosphoenolpyruvate | Escherichia coli |