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Literature summary for 2.5.1.54 extracted from

  • Jossek, R.; Bongaerts, J.; Sprenger, G.A.
    Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli (2001), FEMS Microbiol. Lett., 202, 145-148.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene aroF, Tyr-sensitive isozyme, expression of wild-type and mutants in strain AB3257 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion mutant of Tr-sensitive isozyme, gene aroF, lacking the first 7 amino acid residues of the N-terminus, mutant is insensitive against inhibition by tyrosine Escherichia coli
N8K similar activity and substrate affinities like the wild-type, but insensitive against inhibition by tyrosine, decreased thermostability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Tyr wild-type isozyme, mutant N8K and N-terminal deletion mutant are not inhibited, N-terminus is structurally involved in the sensitivity for feedback inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
Tyr-sensitive isozyme, mutant N8K, gel filtration Escherichia coli
75000
-
Tyr-sensitive isozyme, wild-type, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
K12
-
Escherichia coli
-
tyrosine-sensitive isozyme
-
Escherichia coli W3110 / ATCC 27325
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Tyr-sensitive isozyme, wild-type and mutants from strain AB3257 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30 40 purified Tyr-sensitive isozyme, wild-type and mutant N8K Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli W3110 / ATCC 27325 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
Tyr-sensitive isozyme, wild-type: 50% loss of activity in 80 min in presence of phosphoenolpyruvate, mutant N8K: 50% loss of activity in 20 min in presence of phosphoenolpyruvate Escherichia coli