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Literature summary for 2.5.1.54 extracted from

  • Sheflyan, G.Y.; Howe, D.L.; Wilson, T.L.; Woodard, R.W.
    Enzymatic synthesis of 3-deoxy-D-manno-octulosonate 8-phosphate 3-deoxy-D-altro-octulosonate 8-phosphate, 3,5-dideoxy-D-gluco(manno)-octulosonate 8-phosphate by 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (1998), J. Am. Chem. Soc., 120, 11027-11032.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
phosphoenolpyruvate recombinant enzyme, with D-arabinose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
0.0053
-
phosphoenolpyruvate recombinant enzyme, with erythrose 4-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
0.01
-
phosphoenolpyruvate recombinant enzyme, with ribose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
0.015
-
phosphoenolpyruvate recombinant enzyme, with 2-deoxyribose 5-phosphate as cosubstrate, pH 7.5, 25°C Escherichia coli
0.03
-
D-arabinose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
0.141
-
D-erythrose 4-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
6
-
ribose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli
6.8
-
2-deoxyribose 5-phosphate recombinant enzyme, pH 7.5, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Escherichia coli first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
phenylalanine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from overexpressing strain Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate
-
Escherichia coli 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
-
Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
ir
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-ribose 5-phosphate
-
Escherichia coli 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
DAH7-P synthase (phe)
-
Escherichia coli