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Literature summary for 2.5.1.54 extracted from

  • Suzuki, N.; Sakuta, M.; Shimizu, S.
    Purification and characterization of a cytosolic isozyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from cultured carrot cells (1996), J. Plant Physiol., 149, 19-22.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
D-erythrose 4-phosphate cytosolic isozyme, pH 8.6 Daucus carota

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Daucus carota 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Daucus carota
Mg2+ stimulates Daucus carota
Mn2+ stimulates Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
x * 115000, cytosolic isozyme, SDS-PAGE Daucus carota
400000
-
gel filtration Daucus carota

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Daucus carota first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Daucus carota
-
cytosolic isozyme
-
Daucus carota
-
cv. Kurodagosun
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic isozyme, 79.8fold to homogeneity Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
purified cytosolic isozyme Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Daucus carota 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Daucus carota 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 115000, cytosolic isozyme, SDS-PAGE Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
cytosolic isozyme Daucus carota