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Literature summary for 2.5.1.54 extracted from

  • Bracher, M.; Schweingruber, M.E.
    Purification of the tyrosine inhibitable 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Schizosaccharomyces pombe (1977), Biochim. Biophys. Acta, 485, 446-451.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Tyr at pH 6.2-7.8; Tyr-sensitive isozyme Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no stimulation of purified enzyme Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
tyrosine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
326fold Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.75
-
purified enzyme Schizosaccharomyces pombe

Storage Stability

Storage Stability Organism
0.1 M potassium phosphate, pH 7.4, 1 mM phosphoenolpyruvate, 0.02% NaN3, purified enzyme, stable for 2-3 months Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Schizosaccharomyces pombe 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer
-
Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 7.8
-
Schizosaccharomyces pombe