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Literature summary for 2.5.1.54 extracted from

  • Schoner, R.; Herrmann, K.M.
    3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli (1976), J. Biol. Chem., 251, 5440-5447.
    View publication on PubMed

General Stability

General Stability Organism
phosphoenolpyruvate stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phosphate non-competitive with respect to both phosphoenolpyruvate and D-erythrose 4-phosphate Escherichia coli
Tyr feedback inhibition; noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Escherichia coli
0.0058
-
phosphoenolpyruvate pH 7.0, 37°C Escherichia coli
0.0965
-
D-erythrose 4-phosphate pH 7.0, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, either a rapid monomer-dimer equilibrium or a very asymetric shape for the native enzyme, SDS-PAGE Escherichia coli
66000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
tyrosine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
105fold Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate mechanism Escherichia coli
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered ping-pong bi-bi mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56
-
purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, in phosphate buffer containing phosphoenolpyruvate, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 39000, either a rapid monomer-dimer equilibrium or a very asymetric shape for the native enzyme, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
2 h, in 3(N-morpholono)propane-sulfonic acid buffer, 50% loss of activity Escherichia coli
25
-
1 h, in 1,3-bis[tris(hydroxymethyl)methylamino]propane buffer, 10% loss of activity Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
122
-
phosphoenolpyruvate pH 7.0, 37°C, MW 79000 assumed Escherichia coli
122
-
D-erythrose 4-phosphate pH 7.0, 37°C, MW 79000 assumed Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8.5 pH 5.7: about 25% of maximal activity, pH 8.5: about 30% of maximal activity Escherichia coli