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Literature summary for 2.5.1.32 extracted from

  • Liu, J.X.; Chiou, C.Y.; Shen, C.H.; Chen, P.J.; Liu, Y.C.; Jian, C.D.; Shen, X.L.; Shen, F.Q.; Yeh, K.W.
    RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid (2014), SpringerPlus, 3, 478.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information RNA interference-based gene silencing of phytoene synthase, the OgPSY RNAi construct driven by the 35S promoter is introduced into protocorm-like body (PLB) of Oncidium orchid by Agrobacterium tumefaciens-mediated transformation Oncidium hybrid cultivar

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Oncidium hybrid cultivar 9507
-

Organism

Organism UniProt Comment Textmining
Oncidium hybrid cultivar
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oncidium hybrid cultivar
-
shoot
-
 Oncidium hybrid cultivar
-

Synonyms

Synonyms Comment Organism
PSY
-
 Oncidium hybrid cultivar

General Information

General Information Comment Organism
malfunction RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid. PSY-RNAi in transgenic Oncidium alters the expression pattern of biosynthetic pathway and leads to decreased accumulation of carotenoid contents. The transgenic orchids show downregulated level of enzyme PSY and geranyl synthase gene. They display semi-dwarf phenotype, brilliant green leaves, and development-arrested plastids with rare grana. The total carotenoid content is decreased and the efficiency of the photosynthetic electron transport is declined. The chlorophyll level and the expression of chlorophyll biosynthetic genes, such as OgGLUTR and OgCS are dramatically reduced. The endogenous level of gibberellic acid and abscisic acid in the dwarf transformants are 4fold lower than in wild-type plants. Chilling tolerance of the transgenic Oncidium plants is reduced Oncidium hybrid cultivar
metabolism phytoene synthase is the first rate-limiting regulatory enzyme in the carotenoid biosynthesis pathway Oncidium hybrid cultivar