Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Tetranychus urticae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abamectin | competitive inhibition (38.46% at 0.05 mM) of isoform GSTd14 | Tetranychus urticae | |
bifenthrin | 7.27% inhibition of isoform GSTd10 at 0.05 mM | Tetranychus urticae | |
clofentezine | 28.05% inhibition of isoform GSTd14 at 0.05 mM and 27.68% inhibition of isoform GSTm09 at 0.05 mM | Tetranychus urticae | |
hexythiazox | 11.21% inhibition of isoform GSTd14 at 0.05 mM and 12.94% inhibition of isoform GSTm09 at 0.05 mM | Tetranychus urticae | |
pyridaben | 25.84% inhibition of isoform GSTm09 at 0.05 mM | Tetranychus urticae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae | |
0.33 | - |
glutathione | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
0.84 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
1.69 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae | |
2.34 | - |
glutathione | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae | |
3.79 | - |
glutathione | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetranychus urticae | - |
two-spotted spider mite | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Tetranychus urticae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
isoform GSTd10, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
0.08 | - |
isoform GSTd14, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
0.09 | - |
isoform GSTm09, with 1,2-dicloro-4-nitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
0.1 | - |
isoform GSTd14, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
0.34 | - |
isoform GSTd10, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
0.69 | - |
isoform GSTd14, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
1.1 | - |
isoform GSTd10, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
3.34 | - |
isoform GSTm09, with cumene hydroperoxide as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
15.94 | - |
isoform GSTm09, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C | Tetranychus urticae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dichloro-4-nitrobenzene + glutathione | low activity | Tetranychus urticae | ? | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | best substrate | Tetranychus urticae | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
cumene hydroperoxide + glutathione | - |
Tetranychus urticae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Tetranychus urticae |
GSTD10 | isoform | Tetranychus urticae |
GSTd14 | isoform | Tetranychus urticae |
GSTm09 | isoform | Tetranychus urticae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
glutathione | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae | |
0.027 | - |
glutathione | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
0.03 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae | |
0.051 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
0.25 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae | |
0.39 | - |
glutathione | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
glutathione | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae | |
0.018 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd14, at pH 6.5 and 25°C | Tetranychus urticae | |
0.06 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
0.08 | - |
glutathione | isoform GSTd10, at pH 6.5 and 25°C | Tetranychus urticae | |
0.17 | - |
glutathione | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae | |
0.94 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTm09, at pH 6.5 and 25°C | Tetranychus urticae |