Crystallization (Comment) | Organism |
---|---|
- |
Aeromonas hydrophila |
Protein Variants | Comment | Organism |
---|---|---|
E213A | kcat highly decreased compared to wild-type, Km similar to wild-type | Aeromonas hydrophila |
E215A | kcat highly decreased compared to wild-type, Km (ADP-D-ribosyl)n-actin similar to wild-type, Km (NAD+) increased compared to wild-type | Aeromonas hydrophila |
N86A | kcat moderately decreased compared to wild-type, Km decreased compared to wild-type | Aeromonas hydrophila |
S178A | kcat decreased compared to wild-type, Km increased compared to wild-type | Aeromonas hydrophila |
Y77A | kcat decreased compared to wild-type, Km increased compared to wild-type | Aeromonas hydrophila |
Y82A | kcat decreased compared to wild-type, Km increased compared to wild-type | Aeromonas hydrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol | - |
Aeromonas hydrophila | |
N-(acridin-9-yl)-4-nitrobenzamide | - |
Aeromonas hydrophila | |
N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide | - |
Aeromonas hydrophila | |
suramin | - |
Aeromonas hydrophila | |
V8 protease | - |
Aeromonas hydrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NAD+ | mutant N86A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.005 | - |
NAD+ | mutant E213A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.006 | - |
NAD+ | wild-type, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.008 | - |
NAD+ | mutant Y77A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.011 | - |
(ADP-D-ribosyl)n-actin | mutant N86A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.012 | - |
NAD+ | mutant Y82A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.014 | - |
NAD+ | mutant E215A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.018 | - |
NAD+ | mutant S178A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.02 | - |
(ADP-D-ribosyl)n-actin | mutant E213A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.023 | - |
(ADP-D-ribosyl)n-actin | mutant E215A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.024 | - |
(ADP-D-ribosyl)n-actin | wild-type, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.033 | - |
(ADP-D-ribosyl)n-actin | mutant S178A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.035 | - |
(ADP-D-ribosyl)n-actin | mutant Y82A, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.037 | - |
(ADP-D-ribosyl)n-actin | mutant Y77A, 25°C, pH not specified in the publication | Aeromonas hydrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila | Q49TP5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-actin | VahC is shown to ADP-ribosylate Arg-177 of actin. VahC activity causes depolymerization of actin filaments, which induces caspase-mediated apoptosis in HeLa Tet-Off cells | Aeromonas hydrophila | nicotinamide + (ADP-D-ribosyl)n+1-actin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mART | - |
Aeromonas hydrophila |
mono-ADP-ribosyltransferase | - |
Aeromonas hydrophila |
VahC | - |
Aeromonas hydrophila |
vsdc | - |
Aeromonas hydrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | mutant E215A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.05 | - |
(ADP-D-ribosyl)n-actin | mutant E215A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.11 | - |
NAD+ | mutant E213A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.11 | - |
(ADP-D-ribosyl)n-actin | mutant E213A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.65 | - |
NAD+ | mutant Y82A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.65 | - |
(ADP-D-ribosyl)n-actin | mutant Y82A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
1.3 | - |
NAD+ | mutant Y77A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
1.3 | - |
(ADP-D-ribosyl)n-actin | mutant Y77A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
1.38 | - |
NAD+ | mutant S178, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
1.38 | - |
(ADP-D-ribosyl)n-actin | mutant S178A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
9.35 | - |
NAD+ | mutant N86A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
9.35 | - |
(ADP-D-ribosyl)n-actin | mutant N86A, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
22 | - |
NAD+ | wild-type, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication | Aeromonas hydrophila | |
22 | - |
(ADP-D-ribosyl)n-actin | wild-type, co-substrate: NAD+, 25°C, pH not specified in the publication | Aeromonas hydrophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
suramin | 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.0041 | - |
N-(acridin-9-yl)-4-nitrobenzamide | 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.0041 | - |
N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide | 25°C, pH not specified in the publication | Aeromonas hydrophila | |
0.0075 | - |
2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol | 25°C, pH not specified in the publication | Aeromonas hydrophila |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
25°C, pH not specified in the publication | Aeromonas hydrophila | suramin | |
0.0858 | - |
25°C, pH not specified in the publication | Aeromonas hydrophila | N-(acridin-9-yl)-4-nitrobenzamide | |
0.086 | - |
25°C, pH not specified in the publication | Aeromonas hydrophila | N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide | |
0.157 | - |
25°C, pH not specified in the publication | Aeromonas hydrophila | V8 protease |