Cloned (Comment) | Organism |
---|---|
expression of wild-type FLAG-tagged human HAS1, HAS2, or HAS3, and of HAS T110A mutant enzyme in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T110A | site-directed mutagenesis of the phosphorylation site residue, the mutant is not inhibited by AMP-activated protein kinase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxyglucose | - |
Homo sapiens | |
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside | modulates aortic smooth muscle cell motility and adhesive properties through AMP-activated protein kinase, AMPK | Homo sapiens | |
metformin | modulates aortic smooth muscle cell motility and adhesive properties through AMP-activated protein kinase, AMPK | Homo sapiens | |
additional information | AMP-activated protein kinase, AMPK, phosphorylates HAS2 at Thr110, which inhibits its enzymatic activity, and is itself inhibit by Compound C. Isoenzymes HAS1 and HAS3 are not modified by the kinase | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92819 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | AMP-activated protein kinase, AMPK, phosphorylates HAS2 at Thr110, which inhibits its enzymatic activity. Isoenzymes HAS1 and HAS3 are not modified by the kinase | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aortic smooth muscle cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
Has2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | reduction of HA due to decreased HAS activity, caused by phosphorylation at Thr110 through AMP-activated protein kinase, decreases the ability of aortic smooth muscle cells to proliferate, migrate, and recruit immune cells, thereby reducing the pro-atherosclerotic AoSMC phenotype. AMP-activated protein kinase can block the pro-atherosclerotic signals driven by HA by interaction with its receptors | Homo sapiens |
physiological function | hyaluronan synthesis is inhibited by adenosine monophosphate-activated protein kinase through the regulation of HAS2 activity in human aortic smooth muscle cells | Homo sapiens |