Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of single knock-out of gene GPI2 and double knockout mutants of genes GPI1 and GPI119 from Cancdida albicans strain BWP17, microsomes generated from GPI2 mutants exhibited lower GPI-GnT activity as compared with CAI4, phenotypes, overview | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Candida albicans | 5618 | - |
microsome | - |
Candida albicans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | Candida albicans | - |
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q5A6L6 | enzyme complex subunit GPI2; diverse strains | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | - |
Candida albicans | UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GPI-GnT | - |
Candida albicans |
GPI-N-acetylglucosaminyltransferase | - |
Candida albicans |
GPI2 | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Candida albicans |
General Information | Comment | Organism |
---|---|---|
malfunction | GPI19 mutants show up-regulation of GPI2, whereas GPI2 mutants show upregulation of GPI19. GPI2 disruption leads to defective hyphal morphogenesis due to altered Ras signaling. Reintroduction of GPI2 into the GPI2 heterozygous strain can reverse the phenotypes | Candida albicans |
metabolism | analysis of the regulatory function of the enzyme, overview | Candida albicans |
physiological function | the enzyme complex, GPI-N-acetylglucosaminyltransferase (GPI-GnT), is involved in the first step of GPI anchor biosynthesis in eukaryotes. Candida albicans has several glycosylphosphatidylinositol (GPI)-anchored virulence factors. Inhibiting GPI biosynthesis attenuates its virulence. Enzyme complex subunit GPI2 is essential for growth and hyphal morphogenesis and is needed for filamentation. The GPI-GnT enzyme complex accessory subunits, GPI2 and GPI19, exhibit opposite effects on ergosterol biosynthesis and Ras signaling (which determines hyphal morphogenesis), because the two subunits negatively regulate one another. GPI19 controls ergosterol biosynthesis through ERG11 levels, whereas GPI2 determines the filamentation by cross-talk with Ras1 signaling. GPI2 affects GPI anchor biosynthesis and cell wall | Candida albicans |