Information on EC 2.4.1.198 - phosphatidylinositol N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.198
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
In some species, the long-chain acyl groups of the phosphatidyl group are partly replaced by long-chain alkyl or alk-1-enyl groups.
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:1-phosphatidyl-1D-myo-inositol 6-(N-acetyl-alpha-D-glucosaminyl)transferase
Involved in the first step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. In mammalian cells, the enzyme is composed of at least five subunits (PIG-A, PIG-H, PIG-C, GPI1 and PIG-P). PIG-A subunit is the catalytic subunit. In some species, the long-chain acyl groups of the phosphatidyl group are partly replaced by long-chain alkyl or alk-1-enyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
144388-35-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
afpig-a; strain YJ-407. Most common opportunistic fungal pathogen of human
Swissprot
Manually annotated by BRENDA team
afpig-a; strain YJ-407. Most common opportunistic fungal pathogen of human
Swissprot
Manually annotated by BRENDA team
GPI-GlcNAc transferase subunit P; formerly Aspergillus nidulans
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene CaGPI19, encoding an accessory subunit of the enzyme complex
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain XM37-10c
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
UDP-N-acetyl-D-glucosamine + phosphatidylinositol
UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol
UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates
K+
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activates
Mg2+
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activates
Mn2+
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activates
additional information
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requires cations for activity, such as Mn2+, Mg2+, K+ or Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetic acid
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N-ethylmaleimide
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binding site is close or at the active site, UDP-N-acetyl-D-glucosamine protects; strong
p-Chloromercuriphenylsulfonic acid
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SH-alkylating reagents
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additional information
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GTP-bound Ras2 protein associates in vivo with enzyme complex and inhibits its activity. Ras2 associates with enzyme complex subunit Eri1
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
component 2 of dolichyl-phosphate-mannose synthase
DPM2 protein binds directly to the enzyme complex and enhances the enzyme activity, regulatory role; i.e. DPM2
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additional information
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no activation by Triton X-100 or Triton X-114
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
overexpression of active froms of the Ras family of proteins does not affect glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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CHO-K1 cell lines G9PLAP and mutant G9PLAP.85
Manually annotated by BRENDA team
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5th instar larvae
Manually annotated by BRENDA team
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with paroxysmal nocturnal hemoglobinuria phenotype, i.e. PNH, obtained by Epstein-Barr immortalization of lymphocytes from PNH-patients
Manually annotated by BRENDA team
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of the 5th instar larvae
Manually annotated by BRENDA team
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BW514.3 thymoma cell line
Manually annotated by BRENDA team
additional information
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the wild-type PIGA-1 protein is solely detected in the germline, and no EGFP-tagged PIGA-1 is detected in so­matic cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity purified
purification of GPI1 by overexpression of PIG-A in JY5 cells and isolation of the complex by affinity chromatography; purification of GPI1 by overexpression of PIG-A in JY5 cells and isolation of the complex by affinity chromatography
recombinant FLAG-tagged and His-tagged PIG-A from human B lymphoblastoid JY5 cells and from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression of FLAG-tagged PIG-C, GST-tagged PIG-A and PIG-H with and without mGPI1 in the mGPI1-deficient F9 cells; mGPI1, DNA sequence determination, expression in embryonal carcinoma F9 cells
expressed in Daudi cells. Daudi cells have no PIG-Y transcritpt. PIG-Y cDNA restores the surface expression of CD59, DAF and CD48, but an empty vector does not. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase activity is also restored; expressed in HeLa cells; in chinese hamster ovary cells
expressed in Escherichia coli DH5alpha
expression of FLAG-tagged and His-tagged PIG-A in PIG-A-deficient human B lymphoblastoid JY5 cells and in Escherichia coli, complementation in JY5 cells
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expression of PIG-A/GPI3 and PIG-C/GPI2 as FLAG-tagged fusion proteins in yeast, expression of FLAG-tagged PIG-A/GPI3 in Escherichia coli
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expression of PIG-P in enzyme deficient mouse mutant T cell line can complement and restore the enzyme activity; overexpression of FLAG- and GST-double tagged PIG-A in human B lymphoblastoid JY5 cells
genes involved in GPI-anchor synthesis in Caenorhabditis elegans, overview. Isolation and analysis of piga-1-knockout allele tm2939
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hGPI1, DNA sequence determination, coexpression as GST-tagged protein in human B lymphoblastoid JY25 or JY5 cells with the other three GST- or FLAG-tagged protein of the active protein complex
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
downregulation of ERG11 downregulates CaGPI19 and GPI biosynthesis
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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