Cloned (Comment) | Organism |
---|---|
isoform UGT1A, expression in HEK-293T cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H37L | mutant of isoform UGT1A9, abolishes activity towards all substrates tested except retigabine | Homo sapiens |
H37P | mutant of isoform UGT1A9, abolishes activity towards all substrates tested except retigabine | Homo sapiens |
H37Q | mutant of isoform UGT1A9, abolishes activity towards all substrates tested except retigabine | Homo sapiens |
M33I | mutant of isoform UGT1A9, increase in both Km and Vmax value | Homo sapiens |
M33Q | mutant of isoform UGT1A9, increase in both Km and Vmax value | Homo sapiens |
M33T | mutant of isoform UGT1A9, increase in both Km and Vmax value | Homo sapiens |
M33V | mutant of isoform UGT1A9, increase in both Km and Vmax value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
4-methylumbeliferone | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.014 | - |
sulfinpyrazone | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.0209 | - |
frusemide | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
0.026 | - |
propofol | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.032 | - |
sulfinpyrazone | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.032 | - |
retigabine | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.033 | - |
sulfinpyrazone | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.039 | - |
sulfinpyrazone | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.046 | - |
Mycophenolic acid | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.053 | - |
sulfinpyrazone | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
0.055 | - |
retigabine | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.062 | - |
retigabine | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.068 | - |
retigabine | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
0.07 | - |
propofol | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.087 | - |
Mycophenolic acid | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.099 | - |
retigabine | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.109 | - |
propofol | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.113 | - |
propofol | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.113 | - |
propofol | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
0.129 | - |
frusemide | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.157 | - |
Mycophenolic acid | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.183 | - |
frusemide | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.187 | - |
frusemide | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.2 | - |
Mycophenolic acid | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.228 | - |
4-methylumbeliferone | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.229 | - |
frusemide | wildtype, pH 7.4, 37°C | Homo sapiens | |
0.233 | - |
4-methylumbeliferone | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.234 | - |
4-methylumbeliferone | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.336 | - |
Mycophenolic acid | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
0.507 | - |
(S)-naproxen | mutant M33V, pH 7.4, 37°C | Homo sapiens | |
0.62 | - |
(S)-naproxen | mutant M33I, pH 7.4, 37°C | Homo sapiens | |
0.66 | - |
(S)-naproxen | mutant M33Q, pH 7.4, 37°C | Homo sapiens | |
0.79 | - |
(S)-naproxen | wildtype, pH 7.4, 37°C | Homo sapiens | |
1.013 | - |
(S)-naproxen | mutant M33T, pH 7.4, 37°C | Homo sapiens | |
1.621 | - |
4-methylumbeliferone | mutant M33T, pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-naproxen + UDP-alpha-D-glucuronate | - |
Homo sapiens | beta-D-glucuronosyl (S)-naproxen + UDP | - |
? | |
4-methylumbeliferone + UDP-alpha-D-glucuronate | - |
Homo sapiens | 4-methylumbelliferyl-7-O-alpha-D-glucuronide | - |
? | |
frusemide + UDP-alpha-D-glucuronate | - |
Homo sapiens | frusemide D-glucuronide | - |
? | |
mycophenolic acid + UDP-alpha-D-glucuronate | - |
Homo sapiens | mycophenolic acid D-glucuronide | - |
? | |
propofol + UDP-alpha-D-glucuronate | - |
Homo sapiens | UDP + propofol D-glucuronide | - |
? | |
retigabine + UDP-alpha-D-glucuronate | - |
Homo sapiens | retigabine D-glucuronide | - |
? | |
sulfinpyrazone + UDP-alpha-D-glucuronate | - |
Homo sapiens | sulfinpyrazone D-glucuronide | - |
? |