Cloned (Comment) | Organism |
---|---|
modified N-terminal region, His-tagged version, expressed in Pichia pastoris | Mus musculus |
modified N-terminal region, His-tagged version, expressed in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C369A | improvement of enzyme secretion (expressed in Pichia pastoris) | Homo sapiens |
I73N | artificial glycosylation site | Homo sapiens |
I73N/C369A | further improvement of enzyme secretion (expressed in Pichia pastoris) | Homo sapiens |
I74N | artificial glycosylation site | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzimidazole | competitive | Homo sapiens | |
benzimidazole | competitive | Mus musculus | |
benzylimidazole | competitive | Homo sapiens | |
benzylimidazole | competitive | Mus musculus | |
cysteamine | - |
Mus musculus | |
imidazole | - |
Homo sapiens | |
imidazole | - |
Mus musculus | |
L-glutaminyl-7-amido-4-methylcoumarin | substrate inhibition | Homo sapiens | |
L-glutaminyl-beta-naphthylamide | substrate inhibition | Homo sapiens | |
L-glutaminyl-beta-naphthylamide | substrate inhibition | Mus musculus | |
methylimidazole | - |
Homo sapiens | |
methylimidazole | - |
Mus musculus | |
N,N-dimethylcysteamine | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.03 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.03 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.032 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.035 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.04 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.058 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.06 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.08 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.092 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.1 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.102 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.11 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.16 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.16 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.16 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.23 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.36 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.47 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.61 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi complex | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged version | Homo sapiens |
hydrophobic interaction chromatography, ion exchange chromatography, gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gln-Gln | - |
Mus musculus | pGlu-Gln + NH3 | - |
? | |
Gln-Gln | - |
Homo sapiens | pGlu-Gln + NH3 | - |
? | |
Gln-Glu | - |
Mus musculus | pGlu-Glu + NH3 | - |
? | |
Gln-Glu | - |
Homo sapiens | pGlu-Glu + NH3 | - |
? | |
Gln-Gly | - |
Mus musculus | pGlu-Gly + NH3 | - |
? | |
Gln-Gly | - |
Homo sapiens | pGlu-Gly + NH3 | - |
? | |
Gln-Gly-Pro | - |
Mus musculus | pGlu-Gly-Pro + NH3 | - |
? | |
Gln-Gly-Pro | - |
Homo sapiens | pGlu-Gly-Pro + NH3 | - |
? | |
Gln-Phe-Ala | - |
Mus musculus | pGlu-Phe-Ala + NH3 | - |
? | |
Gln-Phe-Ala | - |
Homo sapiens | pGlu-Phe-Ala + NH3 | - |
? | |
Gln-Tyr-Ala | - |
Mus musculus | pGlu-Tyr-Ala + NH3 | - |
? | |
Gln-Tyr-Ala | - |
Homo sapiens | pGlu-Tyr-Ala + NH3 | - |
? | |
L-glutaminyl-7-amido-4-methylcoumarin | - |
Mus musculus | pGlu-7-amido-4-methylcoumarin + NH3 | - |
? | |
L-glutaminyl-7-amido-4-methylcoumarin | - |
Homo sapiens | pGlu-7-amido-4-methylcoumarin + NH3 | - |
? | |
L-glutaminyl-beta-naphthylamide | - |
Mus musculus | pGlu-beta-naphthylamide + NH3 | - |
? | |
L-glutaminyl-beta-naphthylamide | - |
Homo sapiens | pGlu-beta-naphthylamide + NH3 | - |
? | |
QEDL | - |
Mus musculus | pEEDL + NH3 | - |
? | |
QEDL | - |
Homo sapiens | pEEDL + NH3 | - |
? | |
QEYF | - |
Mus musculus | pEEYF + NH3 | - |
? | |
QEYF | - |
Homo sapiens | pEEYF + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutaminyl cyclase | isoenzyme | Mus musculus |
glutaminyl cyclase | isoenzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
1.65 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.6 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.7 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
3.3 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
3.4 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
4 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
4.57 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
6.4 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
6.4 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
6.98 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
7.5 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
7.7 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
7.79 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
8.66 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
11.4 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
11.78 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
17.48 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
22.9 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
24.1 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | for kcat/KM | Mus musculus |
7.5 | 8 | for kcat/KM | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
benzylimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.0062 | - |
benzylimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.027 | - |
N,N-dimethylcysteamine | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.052 | - |
methylimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.069 | - |
cysteamine | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.082 | - |
methylimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.103 | - |
imidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.124 | - |
benzimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
0.235 | - |
imidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
0.25 | - |
benzimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
1.57 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
3.55 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
4.47 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
9 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
16 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
28 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
38 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
54 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
55 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
95 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
103 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
104 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
111 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
117 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
123 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
207 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
229 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
311 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
394 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
403 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
413 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
554 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus |