Information on EC 2.3.2.5 - glutaminyl-peptide cyclotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.3.2.5
-
RECOMMENDED NAME
GeneOntology No.
glutaminyl-peptide cyclotransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
general acid/base working mechanism, pivoting around a strictly conserved glutamate
-
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
DFT theoretical investigation on the catalytic mechanism of glutaminyl cyclase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
aminoacyl group transfer
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutaminyl-peptide gamma-glutamyltransferase (cyclizing)
Involved in the formation of thyrotropin-releasing hormone and other biologically active peptides containing N-terminal pyroglutamyl residues. The enzyme from papaya also acts on glutaminyl-tRNA.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cyclotransferase, glutaminyl-transfer ribonucleate
-
-
-
-
glutamine cyclotransferase
-
glutaminyl cyclase
-
-
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
isoenzyme
glutaminyl cyclase
-
glutaminyl cyclase
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
isoenzyme
glutaminyl cyclase
-
glutaminyl cyclase
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
glutaminyl cyclase
-
glutaminyl cyclase
Xanthomonas campestris ATCC33913
-
-
glutaminyl cyclase
-
glutaminyl-tRNA cyclotransferase
-
-
-
-
Golgi-resident glutaminyl cyclase
-
h-isoQC
-
-
QC
-
-
-
-
sQC
-
secretory glutaminyl cyclase
CAS REGISTRY NUMBER
COMMENTARY
37257-21-9
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; expressed in Pichia pastoris
-
-
Manually annotated by BRENDA team
isoform DromeQC
SwissProt
Manually annotated by BRENDA team
isoform isoDromeQC
UniProt
Manually annotated by BRENDA team
expressed in Pichia pastoris
Uniprot
Manually annotated by BRENDA team
neonatal
-
-
Manually annotated by BRENDA team
expressed in Pichia pastoris
-
-
Manually annotated by BRENDA team
Xanthomonas campestris ATCC33913
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
glutaminyl cyclase contributes to the formation of focal and diffuse diglutamate-Abeta peptide deposits in hippocampus
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
39SrpL21 protein
?
show the reaction diagram
-
-
-
?
Acp33A protein
?
show the reaction diagram
-
-
-
?
ALDH2 protein
?
show the reaction diagram
-
-
-
?
amyloid beta(3-40)
pyroglutamyl-amyloid beta(3-40) + NH3
show the reaction diagram
-
-
-
?
amyloid peptide Abeta(3-40,42)
Abeta(3[pE]-40,42) + H2O
show the reaction diagram
-
-
-
?
amyloid peptide Abeta(3-40/42)
pGluAbeta(3-40/42) + H2O
show the reaction diagram
-
-
significant portion of Abeta peptides of amyloid plaques in Alzheimer's disease brains
?
DIM-10 protein
?
show the reaction diagram
-
-
-
?
EFRHHDSGYE-NH2
pEFRHHDSGYE-NH2 + H2O
show the reaction diagram
-
-
-
?
Gln
L-5-oxoproline + NH3
show the reaction diagram
-
-
-
?
Gln
pyroglutamate + NH3
show the reaction diagram
-
-
-
?
Gln(3)-amyloid-beta peptide 3-11 amide
?
show the reaction diagram
-
-
-
-
?
Gln(3)-amyloid-beta peptide 3-21 amide
?
show the reaction diagram
-
-
-
-
?
Gln(3)-amyloid-beta peptide 3-40 amide
?
show the reaction diagram
-
-
-
-
?
Gln-2-naphthylamide
pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
Gln-2-naphthylamide
2-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
Gln-4-methylcoumarinylamide
2-oxoprolyl-4-methylcoumarinylamide + NH3
show the reaction diagram
-
-
-
?
Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
-
?
Gln-7-amino-4-methylcoumarin
pyroglutamic acid-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
Gln-7-amino-4-methylcoumarin
pyroglutamic acid-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Ala
pyroglutamic acid-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Ala-Ala-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Ala-Ala-Ser-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-Ser-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Arg-Gly-Ile-NH2
pyroglutamic acid-Arg-Gly-Ile-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Arg-Tyr-Phe-NH2
pyroglutamic acid-Arg-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Asn-Gly-Ile-NH2
pyroglutamic acid-Asn-Gly-Ile-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-beta-naphthylamide
pyroglutamic acid-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
?
Gln-beta-naphthylamide
pyroglutamic acid-beta-naphthylamine + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
L-5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
pyroglutamic acid-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
pyroglutamic acid-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
pyroglutamic acid-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
pyroglutamyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
pyroglutamyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
isoDromeQC
-
?
Gln-Gln
pGlu-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
?
Gln-Gln-Tyr-Phe-NH2
pyroglutamic acid-Gln-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu
Glp-Glu + NH3
show the reaction diagram
-
-
-
?
Gln-Glu
Glp-Glu + NH3
show the reaction diagram
-
-
-
?
Gln-Glu
pyroglutamic acid-Glu + NH3
show the reaction diagram
-
-
-
?
Gln-Glu
pGlu-Glu + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Ala-Ala-NH2
pyroglutamic acid-Glu-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Ala-Phe-NH2
pyroglutamic acid-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Asp-Leu-NH2
pyroglutamic acid-Glu-Asp-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Tyr-Ala-NH2
pyroglutamic acid-Glu-Tyr-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Tyr-NH2
pyroglutamic acid-Glu-Tyr-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Glu-Tyr-Phe-NH2
pyroglutamic acid-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
pyroglutamic acid-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
?
Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
isoDromeQC
-
?
Gln-Gly
pGlu-Gly + NH3
show the reaction diagram
-
-
-
?
Gln-Gly-Pro
Glp-Gly-Pro + NH3
show the reaction diagram
-
-
-
?
Gln-Gly-Pro
pyroglutamic acid-Gly-Pro + NH3
show the reaction diagram
-
-
-
?
Gln-Gly-Pro
pGlu-Gly-Pro + NH3
show the reaction diagram
-
-
-
?
Gln-His-Pro
pyroglutamyl-His-Pro
show the reaction diagram
-
-
-
?
Gln-His-Pro-NH2
L-5-oxoprolyl-His-Pro-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-His-Tyr-Phe-NH2
pyroglutamic acid-His-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Lys-Arg-Leu-NH2
pyroglutamic acid-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
?
Gln-Phe-Ala
pGlu-Phe-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Phe-Ala-NH2
pyroglutamic acid-Phe-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Pro-Tyr-Phe-NH2
pyroglutamic acid-Pro-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Ser-Tyr-Phe-NH2
pyroglutamic acid-Ser-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
Gln-Trp-Ala-NH2
pyroglutamic acid-Trp-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
Gln-Tyr
pyroglutamic acid-Tyr + NH3
show the reaction diagram
-
-
-
?
Gln-Tyr-Ala
pyroglutamic acid-Tyr-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Tyr-Ala
pGlu-Tyr-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Tyr-Ala-OH
L-5-oxoprolyl-Tyr-Ala + NH3
show the reaction diagram
-
-
-
?
Gln-Val
Glp-Val + NH3
show the reaction diagram
-
-
-
?
Gln-Val
pyroglutamic acid-Val + NH3
show the reaction diagram
-
-
-
?
Glu-2-naphthylamide
?
show the reaction diagram
-
-
-
-
?
glucagon(3-29)
?
show the reaction diagram
-
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
? + NH3
show the reaction diagram
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
? + NH3
show the reaction diagram
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Glu-Tyr-Ala-NH2
5-oxoprolyl-Glu-Tyr-Ala-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-NH2
? + NH3
show the reaction diagram
-
-
-
?
H-Gln-NH2
? + NH3
show the reaction diagram
-
-
?
H-Gln-Phe-Ala-NH2
5-oxoprolyl-Phe-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Phe-Ala-NH2
5-oxoprolyl-Phe-Ala-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Phe-Ala-OH
5-oxoprolyl-Phe-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Phe-Ala-OH
5-oxoprolyl-Phe-Ala-OH + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Phe-Arg-His-NH2
5-oxoprolyl-Phe-Arg-His-NH2 + NH3
show the reaction diagram
-
-
-
?
H-Gln-Ser-Tyr-Phe-NH2
5-oxoprolyl-Ser-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
?
H-Gln-Tyr-Ala-OH
5-oxoprolyl-Tyr-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Tyr-Ala-OH
5-oxoprolyl-Tyr-Ala-OH + NH3
show the reaction diagram
isoDromeQC
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
isoDromeQC
-
?
L-Gln(3)-amyloid-beta peptide 3-42
L-pyroglutamyl(3)-amyloid-beta peptide 3-42 + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
reaction in cell supernatant is exclusively enzyme-catalyzed
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Xanthomonas campestris ATCC33913
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
pyroglutamate-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
?
L-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
?
L-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
isoDromeQC
-
?
L-Gln-amide
5-oxoprolyl-amide + NH3
show the reaction diagram
-
-
?
L-Gln-amide
5-oxoprolyl-amide + NH3
show the reaction diagram
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
-
-
-
?
L-Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
-
-
L-Gln-Gly
5-oxoprolylglycine + NH3
show the reaction diagram
-
-
?
L-Gln-Gly
5-oxoprolylglycine + NH3
show the reaction diagram
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Arg-Gly-L-Ile-NH2
pyroglutamyl-L-Arg-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Asn-Gly-L-Ile-NH2
L-pyroglutamyl-L-Asn-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Asp-L-Glu-L-Leu-NH2
pyroglutamyl-L-Asp-L-Glu-L-Leu-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Asp-L-Glu-L-Leu-NH2
pyroglutamyl-L-Asp-L-Glu-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
-
-
?
L-Gln-L-Gln-OH
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu-L-Ala-L-Phe-NH2
pyroglutamyl-L-Glu-L-Ala-L-Phe-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu-L-Ala-L-Phe-NH2
pyroglutamyl-L-Glu-L-Ala-L-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
pyroglutamic acid-L-Glu-L-Tyr-L-Ala-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
pyroglutamyl-L-Glu-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
pyroglutamyl-L-Glu-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Glu-OH
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu
5-oxoprolyl-L-Lys-L-Arg-L-Leu + NH3
show the reaction diagram
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
pyroglutamyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
pyroglutamyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
5-oxoprolyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
L-Gln-L-Phe-L-Ala
5-oxoprolyl-L-Phe-L-Ala + NH3
show the reaction diagram
-
-
?
L-Gln-L-Phe-L-Ala-NH2
L-pyroglutamyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Phe-L-Ala-NH2
L-pyroglutamyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
pyroglutamic acid-L-Ser-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Thr-Gly-L-Ile-NH2
L-pyroglutamyl-L-Thr-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Trp-L-Ala-NH2
L-pyroglutamyl-L-Trp-L-Ala-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Val-L-Ala-NH2
L-pyroglutamyl-L-Val-L-Ala-NH2 + NH3
show the reaction diagram
-
-
?
L-Gln-L-Val-L-Ala-NH2
L-pyroglutamyl-L-Val-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
?
L-Gln-tert-butyl ester
L-pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
?
L-Gln-tert-butyl ester
L-pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
L-Gln-tert-butyl ester
5-oxoprolyl-tert-butyl ester
show the reaction diagram
-
-
?
L-Gln-tert-butyl ester
5-oxoprolyl-tert-butyl ester
show the reaction diagram
-
-
?
L-Glu(3)-amyloid-beta peptide 3-42
L-pyroglutamyl(3)-amyloid-beta peptide 3-42 + H2O
show the reaction diagram
-
-
-
?
L-glutamine tert-butyl ester
L-pyroglutamate tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
L-glutamine tert-butyl ester
L-pyroglutamate tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
L-glutamine tert-butyl ester
L-pyroglutamate tert-butyl ester + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-2-naphthylamide
L-5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-4-methylcoumarinylamide
L-5-oxoprolyl-4-methylcoumarinylamide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-beta-naphthylamide
pGlu-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
simple intramolecular cyclization. The mechanism consists of the following main steps: 1. intramolecular nucleophilic attack on the gamma-C=O carbon by the nitrogen of the alpha-amino group, 2. transfer of a proton from the alpha-amino group to the nitrogen of the amide group, facilitated by an acidic group of the enzyme, 3. expulsion of the ammonia-leaving group promoted by this or another acidic enzyme group
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
Gln-His-Pro-Glythyrotropin-releasing hormone, gonadotropin-releasing hormone and L-Gln-Tyr-Ala
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the N-terminal formation of 5-oxoproline is a common post-translational event during biosynthesis of a number of peptides
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the enzyme may participate in the posttranslational processing of hormonal precursors to pyroglutamyl peptides
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
involved in posttranslational modification of the N-terminal glutamine of peptide hormones or neurotransmitters, such as thyrotropin releasing hormone, luteinizing hormone releasing hormone, gastrin, heavy chain of gamma-globulin
-
?
NPLP4 protein
?
show the reaction diagram
-
-
-
?
QAAE
5-oxoprolyl-AAE + NH3
show the reaction diagram
-
-
?
QAAE
5-oxoprolyl-AAE + NH3
show the reaction diagram
-
-
?
QAAF
5-oxoprolyl-AAF + NH3
show the reaction diagram
-
-
?
QAAF
5-oxoprolyl-AAF + NH3
show the reaction diagram
-
-
?
QAAR
5-oxoprolyl-AAR + NH3
show the reaction diagram
-
-
?
QAEA
5-oxoprolyl-AEA + NH3
show the reaction diagram
-
-
?
QAEA
5-oxoprolyl-AEA + NH3
show the reaction diagram
-
-
?
QAFA
5-oxoprolyl-AFA + NH3
show the reaction diagram
-
-
?
QAFA
5-oxoprolyl-AFA + NH3
show the reaction diagram
-
-
?
QARA
5-oxoprolyl-ARA + NH3
show the reaction diagram
-
-
?
QARA
5-oxoprolyl-ARA + NH3
show the reaction diagram
-
-
?
QEAA
5-oxoprolyl-EAA + NH3
show the reaction diagram
-
-
?
QEAA
5-oxoprolyl-EAA + NH3
show the reaction diagram
-
-
?
QEDL
pEEDL + NH3
show the reaction diagram
-
-
-
?
QEYF
pEEYF + NH3
show the reaction diagram
-
-
-
?
QFAA
5-oxoprolyl-FAA + NH3
show the reaction diagram
-
-
?
QFAA
5-oxoprolyl-FAA + NH3
show the reaction diagram
-
-
?
QFRH-NH2
pEFRH-NH2 + H2O
show the reaction diagram
-
-
-
?
QFRH-NH2
pEFRH-NH2 + NH3
show the reaction diagram
-
-
-
?
QGGG
5-oxoprolyl-GGG + NH3
show the reaction diagram
-
-
?
QGGG
5-oxoprolyl-GGG + NH3
show the reaction diagram
-
-
?
QRAA
5-oxoprolyl-RAA + NH3
show the reaction diagram
-
-
?
QRAA
5-oxoprolyl-RAA + NH3
show the reaction diagram
-
-
?
rho-7 protein
?
show the reaction diagram
-
-
-
?
[Gln1]-fertilization promoting peptide
[pyroglutamyl]-fertilization promoting peptide + NH3
show the reaction diagram
-
-
-
?
[Gln1]-gastrin
[pyroglutamyl]-gastrin + NH3
show the reaction diagram
-
-
-
?
[Gln1]-gonadotropin releasing-hormone
[pyroglutamyl]-gonadotropin releasing-hormone + NH3
show the reaction diagram
-
-
-
?
[Gln1]-neurotensin
[pyroglutamyl]-neurotensin + NH3
show the reaction diagram
-
-
-
?
[Gln1]-thyrotropin releasing-hormone
[pyroglutamyl]-thyrotropin releasing-hormone + NH3
show the reaction diagram
-
-
-
?
MCCB protein
?
show the reaction diagram
-
-
-
?
additional information
?
-
-
Cys residues do not participate to the catalytic events
-
-
-
additional information
?
-
-
the second amino acid residue in N-terminal glutaminyl peptides significantly accelerates activity while the third residue provides no further rate enhancement. Substrate binding is the main specificity-determining step
-
-
-
additional information
?
-
-
no substrates are D-Gln-Tyr-Ala and Lys-Arg-Gln-His-Pro-Gly-Lys-Arg, i.e. thyrotropin releasing hormone precursor
-
-
-
additional information
?
-
-
the enzyme may play a key role in posttranslational modification
-
-
-
additional information
?
-
-
enzyme is important during cellular maturation of L-pyroglutamyl-containing peptides
-
-
-
additional information
?
-
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
highest specificities are observed with substrates possessing large hydrophobic residues adjacent to the N-terminal glutamine and for fluorogenic dipeptide surrogates. Enzyme also catalyzes the conversion of N-terminal glutamic acid residues to pyroglutamic acid, but with about 100000fold lower specificity
-
-
-
additional information
?
-
-
highest specificities are observed with substrates possessing large hydrophobic residues adjacent to the N-terminal glutamine and for fluorogenic dipeptide surrogates. Enzyme also catalyzes the conversion of N-terminal glutamic acid residues to pyroglutamic acid, but with about 100000fold lower specificity
-
-
-
additional information
?
-
-
catalyzes the formation of amyloid-beta3(pE)-40/42 after amyloidogenic processing of amyloid precursor protein
-
-
-
additional information
?
-
the enzyme shows no activity towards L-Gln-Gly
-
-
-
additional information
?
-
the enzyme shows no activity towards QAAR
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
39SrpL21 protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
Acp33A protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
ALDH2 protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
DIM-10 protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the N-terminal formation of 5-oxoproline is a common post-translational event during biosynthesis of a number of peptides
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the enzyme may participate in the posttranslational processing of hormonal precursors to pyroglutamyl peptides
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
involved in posttranslational modification of the N-terminal glutamine of peptide hormones or neurotransmitters, such as thyrotropin releasing hormone, luteinizing hormone releasing hormone, gastrin, heavy chain of gamma-globulin
-
?
NPLP4 protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
rho-7 protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
MCCB protein
?
show the reaction diagram
Q86PD7, Q9VRQ9
-
-
-
?
additional information
?
-
-
the enzyme may play a key role in posttranslational modification
-
-
-
additional information
?
-
-
enzyme is important during cellular maturation of L-pyroglutamyl-containing peptides
-
-
-
additional information
?
-
A7ISW1
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
A7ISW2
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
contains a calcium ion
Co2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be restored partially by Co2+ or Mn2+
Zinc
-
contains one zinc ion per protein molecule
Zinc
stoichiometric amounts of zinc bound to protein. Depletion of zinc has no significant effect on protein structure, metal has a catalytic role
Zn
equimolar protein-bound zinc
Zn2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA
Zn2+
-
the active site is coordinated to three conserved residues and one water molecule, which is replaced by an imidazole nitrogen upon binding of inhibitor
Zn2+
-
-
Zn2+
-
the enzyme contains a catalytic zinc ion
Zn2+
contains zinc; contains zinc
Zn2+
the enzyme contains one zinc ion; the enzyme contains one zinc ion
Mn2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be restored partially by Co2+ or Mn2+
additional information
-
substrate specificity for substrates with uncharged backbone and for Gln-Ala and Gln-Glu, does not change significantly by salt addition. With the positively charged substrates Gln-Arg-Gly-Ile-NH2 and Gln-Lys-Arg-Leu-NH2 addition of salt reveals a positive effect on catalysis
additional information
-
substrate specificity for substrates with uncharged backbone does not change significantly by salt addition. For substrates such as Gln-Ala and Gln-Glu, addition of KCl decreases specificity. With the positively charged substrates Gln-Arg-Gly-Ile-NH2 and Gln-Lys-Arg-Leu-NH2 addition of salt reveals a positive effect on catalysis
additional information
-
human glutaminyl cyclase and bacterial zinc aminopeptidase share a common fold and active site. Glutaminyl cyclase does not appear to require zinc for enzymatic activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(3,4-dichlorophenyl)-2-cyano-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(3,4-dimethoxyphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-(trifluoromethyl)phenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-isopropylphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-methoxyphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(cyclopropylmethyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-methylguanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-phenylguanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-3-(4-bromophenyl)-2-cyanoguanidine
-
-
(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
1,10-phenanthroline
-
2 mM, complete inhibition
1,10-phenanthroline
-
inactivated enzyme can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA, little reactivation by Co2+ and Mn2+
1,10-phenanthroline
time-dependent inactivation
1,10-phenanthroline
-
-
1,4-bis-(imidazol-1-yl)methyl-2,5-dimethylbenzene
-
-
1-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(2,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(2-oxo-2-phenylethyl)-imidazole
-
-
1-(3,4-dimethoxybenzyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(2-[1-(1H-imidazol-1-yl)cyclopropyl]ethyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[(3R)-3-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[(3S)-3-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
i.e. P150/03, complete inhibition at 0.01 mM
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]urea
-
-
1-(3,4-dimethoxyphenyl)-3-[4-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-N-(3-(4-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
1-(3,4-dimethoxyphenyl)-N-(3-(5-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
1-(3,5-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3-aminopropyl)-imidazole
-
-
1-(4-acetylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(4-ethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(4-ethylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(6-phenoxyhexyl)-1H-imidazole
-
-
1-Benzylimidazole
-
0.14 mM, 58% inhibition
1-Benzylimidazole
; isoDromeQC
1-Benzylimidazole
-
-
1-Benzylimidazole
;
1-methyl-4-(beta-aminoethyl)-imidazole
-
-
1-methyl-5-(beta-aminoethyl)-imidazole
-
-
1-Methylimidazole
; isoDromeQC
1-Methylimidazole
-
-
1-Methylimidazole
-
-
1-vinylimidazole
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(1-naphthyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3,4,5-trimethoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3-methoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methylphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-[4-(methylthio)phenyl]thiourea
-
-
1-[4-(benzyloxy)phenyl]-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
2,3-dihydro-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
2,3-dihydro-6-methyl-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-5-phenyl-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
2,6-dipicolinic acid
time-dependent inactivation
2-aminobenzimidazole
-
-
2-cyano(3,4,5-trimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
2-cyano(3-(5-methyl-1H-imidazol-1-yl)propyl)-3-(3,4-dimethylphenyl)guanidine
-
-
2-cyano-1-[3-(5-methyl-1H-imidazol-1-yl)propyl]-4-phenylbenzene-1-guanidine
-
-
2-ethyl-4-methyl-imidazole
-
-
2-mercaptoethanol
-
2-methyl-benzylimidazole
-
-
3,5-diamino-1,2-S4-triazole
-
0.14 mM, 10% inhibition
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-2-thioxoquinazolin-4(1H)-one
-
-
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-7-methyl-2-thioxothieno[3,2-d]pyrimidin-4(1H)-one
-
-
3-Amino-1,2,4-triazole
-
0.14 mM, 15% inhibition
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-1,2,3,5,6,7-hexahydro-4H-cyclopenta[4,5]thieno[2,3-d]pyrimidin-4-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]thieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]-thieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
4-(2-imidazol-1-yl-ethoxy)-benzoic acid
-
-
4-imidazole-carboxaldehyde
-
-
4-methylimidazole
-
4-phenyl-1,2,4-triazoleine-3,5-dione
-
0.14 mM, 22% inhibition
5-(methylthio)-1-H-tetrazole
-
0.14 mM, 11% inhibition
5-amino-3H-imidazole-4-carboxylic acid amide
-
-
5-hydroxymethyl-4-methyl-imidazole
-
-
6-benzyl-2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
6-methylpterin
-
-
8-hydroxyquinoline
time-dependent inactivation
ascorbate
-
-
benzimidazole
-
-
benzimidazole
; isoDromeQC
benzimidazole
-
-
benzimidazole
-
competitive
benzimidazole
-
-
benzylimidazole
-
-
benzylimidazole
-
competitive
benzylimidazole
-
-
benzylimidazole
-
-
cacodylate
;
Citric acid
inactivation at pH 5.5, Zn2+ protects
CuCl2
-
0.1 mM
cysteamine
competitive
cysteamine
; isoDromeQC
diethyl dicarbonate
-
rapid inactivation by modification of three essential His residues, at neutral pH, partial reactivation with hydroxylamine
dipicolinic acid
-
inactivated enzyme can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA, little reactivation by Co2+ and Mn2+
EDTA
-
-
EFRH-NH2
-
competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate
EFRHHDSGYE-NH2
-
competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate
ethanolamine
-
ethyl-1H-tetrazole-4-acetate
-
0.14 mM, 9% inhibition
ethylamine
-
ethylenediamine
-
ethylmercaptane
-
FADH2
-
2 mM
Gln-tert-butyl ester
-
-
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-Gln-beta-naphthylamide
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-His-Trp-OH
-
-
imidazol-4-carbonic acid methylester
-
-
imidazole
; isoDromeQC
imidazole
-
-
L-Gln-2-naphthylamide
substrate inhibition
L-glutamine-4-nitroanilide
substrate inhibition; substrate inhibition, isoDromeQC
L-glutaminyl-7-amido-4-methylcoumarin
-
substrate inhibition
L-glutaminyl-beta-naphthylamide
-
substrate inhibition
L-histamine
-
-
L-histidinamide
-
-
L-histidine
-
-
L-Histidinol
-
-
methylimidazole
-
-
methylimidazole
-
-
N,N-dimethylcysteamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-3,4-dimethoxy-benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)-benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chlorobenzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-methoxybenzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
N-(1-(3-(4-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)benzenamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chloro-benzenamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)cyclohexanamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
N-(3-(1H-imidazol-1-yl)propyl)-N-cyclohexyl-2-nitroethene-1,1-diamine
-
-
N-(3-(1H-imidazol-1-yl)propyl)-N-methyl-2-nitroethene-1,1-diamine
-
-
N-(4-chlorophenyl)-N'-[2-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
-
N-(trimethylsilyl)-imidazole
-
-
N-Acetylimidazole
-
-
N-benzoylimidazole
-
-
N-diethylcysteamine
-
N-diethylcysteamine
;
N-dimethylcysteamine
-
N-dimethylcysteamine
; isoDromeQC
N-dimethylcysteamine
-
-
N-methylimidazole
-
-
N-omega-acetylhistamine
-
0.14 mM, 33% inhibition
N-omega-acetylhistamine
; isoDromeQC
N-omega-acetylhistamine
-
-
N-[3-(1H-imidazol-1-yl)propyl]-5-methoxy-1,3-benzothiazol-2-amine
-
-
N-[3-(1H-imidazol-1-yl)propyl]-6-methoxy-1,3-benzothiazol-2-amine
-
-
NEM
-
-
NH4Cl
-
1.0 M
NiCl2
-
0.1 mM
nitron
-
0.14 mM, 39% inhibition
Nomega-acetylhistamine
-
-
Nomega-acetylhistamine
;
Nomega-acetylhistamine
;
oxalic acid diimidazolidide
-
-
P150/03
; isoDromeQC
-
PBD150
-
-
-
PBD150
-
-
-
PBD150
-
highly potent inhibitor
-
PBD150
potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea; potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea
-
PBD150
-
potent inhibitor
-
ZnCl2
-
0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6
EFRH-NH2
-
at pH 6.5, 10times higher compared to KM-value of Gln substrate
4.6
EFRHHDSGYE-NH2
-
-
16
Gln
-
-
0.087
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, in absence of DMSO
-
0.155
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, 1% DMSO
-
0.162
Gln(3)-amyloid-beta peptide 3-21 amide
-
pH 6.0, 30C, 1% DMSO
-
0.089
Gln(3)-amyloid-beta peptide 3-40 amide
-
pH 6.0, 30C, 1% DMSO
-
0.058
Gln-2-naphthylamide
-
pH 8.0, 25C, wild-type enzyme
0.125
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248A
0.161
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme H319L
0.174
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160A
0.254
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305N
0.33
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248Q
0.431
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305E
0.615
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160G
0.736
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme E201D
1.1
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305A
0.054
Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
0.042
Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.143
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
0.158
Gln-Ala
-
pH 8.0, 30C
0.21
Gln-Ala
-
-
0.232
Gln-Ala
-
pH 8.0, 30C
0.357
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
1.3
Gln-Ala
-
-
0.065
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
0.197
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
0.087
Gln-Ala-Ala-NH2
-
pH 8.0, 30Cl
0.164
Gln-Ala-Ala-NH2
-
pH 8.0, 30Cl
0.079
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
0.216
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
0.065
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.091
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.123
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
0.143
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
0.055
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
0.124
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
0.153
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
0.172
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
0.07
Gln-beta-naphthylamide
-
pH 8.0, 30C
0.038
Gln-beta-naphthylamine
-
pH 8.0, 30C
0.044
Gln-Gln
-
pH 8.0, 30C
0.09
Gln-Gln
-
-
0.092
Gln-Gln
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.11
Gln-Gln
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.148
Gln-Gln
-
pH 8.0, 30C
0.59
Gln-Gln
-
pH 8.0, mutant enzyme W207F
0.6
Gln-Gln
-
-
0.63
Gln-Gln
-
pH 8.0, wild-type enzyme
0.64
Gln-Gln
-
-
0.7
Gln-Gln
-
-
0.76
Gln-Gln
-
pH 8.0, mutant enzyme R54W
0.79
Gln-Gln
-
pH 7.0, wild-type enzyme
0.82
Gln-Gln
-
pH 8.0, mutant enzyme F146A
0.9
Gln-Gln
-
pH 7.5, wild-type enzyme; pH 8.5, wild-type enzyme
1.16
Gln-Gln
-
pH 8.0, mutant enzyme Q304L
1.47
Gln-Gln
-
pH 8.0, mutant enzyme K144A
1.77
Gln-Gln
-
pH 8.0, mutant enzyme W207L
2.06
Gln-Gln
-
pH 8.8, wild-type enzyme
4.67
Gln-Gln
-
pH 8.0, mutant enzyme F325A
12.62
Gln-Gln
-
pH 8.0, mutant enzyme E201D
29.53
Gln-Gln
-
pH 8.0, mutant enzyme W329A
0.31
Gln-Gln-Gln
-
-
0.53
Gln-Gln-Gln
-
-
0.148
Gln-Gln-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.041
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
0.045
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
0.094
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
0.106
Gln-Glu
-
pH 8.0, 30C
0.17
Gln-Glu
-
-
0.359
Gln-Glu
-
pH 8.0, 30C
0.47
Gln-Glu
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.607
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
0.61
Gln-Glu
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.077
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
0.102
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
0.039
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.081
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.055
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
0.061
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.094
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.107
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
0.069
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
0.113
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
0.079
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
0.103
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
0.047
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.1
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.16
Gln-Gly
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.247
Gln-Gly
-
pH 8.0, 30C
0.36
Gln-Gly
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.641
Gln-Gly
-
pH 8.0, 30C
1.1
Gln-Gly
-
-
1.88
Gln-Gly
-
-
0.102
Gln-Gly-Pro
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.13
Gln-Gly-Pro
-
pH 8.0, 30C
0.23
Gln-Gly-Pro
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.333
Gln-Gly-Pro
-
pH 8.0, 30C
0.09
Gln-His-Pro-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.068
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
0.092
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
0.034
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.053
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.054
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.118
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.401
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.409
Gln-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.409
Gln-NH2
-
pH 8.0, 30C
0.433
Gln-NH2
-
pH 8.0, 30C
0.446
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
1.1
Gln-NH2
-
wild-type enzyme
1.3
Gln-NH2
-
-
1.4
Gln-NH2
-
-
4.3
Gln-NH2
-
mutant enzyme H307Q
4.9
Gln-NH2
-
mutant enzyme H319Q
7.15
Gln-NH2
-
-
0.06
Gln-Phe-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.1
Gln-Phe-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.069
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.111
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.149
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
18.89
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
0.055
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
0.135
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
0.223
Gln-tert-butyl ester
-
pH 8.0, 30C
1.235
Gln-tert-butyl ester
-
pH 8.0, 30C
4.1
Gln-tert-butyl ester
-
-
6.7
Gln-tert-butyl ester
-
-
0.132
Gln-Thr-Ala
-
-
0.05
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
0.056
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.072
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.078
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
0.211
Gln-Tyr
-
pH 8.0, 30C
0.058
Gln-Tyr-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.063
Gln-Tyr-Ala
-
pH 8.0, 30C
0.08
Gln-Tyr-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.101
Gln-Tyr-Ala
-
pH 8.0, 30C
0.101
Gln-Tyr-Ala-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.153
Gln-Tyr-Ala-OH
-
37C, recombinant enzyme, expressed in Pichia pastoris
0.12
Gln-Val
-
-
0.196
Gln-Val
-
pH 8.0, 30C
0.019
glucagon(3-29)
-
pH 6.0, 30C, 1% DMSO
-
0.32
glutamine tert-butyl ester
-
-
0.063
gonadotropin releasing hormone
-
-
-
0.017
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.08
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.105
H-Gln-7-amino-4-methylcoumarin
pH 8.0, 30C
0.294
H-Gln-Ala-OH
-
pH 8.0, 30C
1.838
H-Gln-Ala-OH
pH 8.0, 30C
2.8
H-Gln-Ala-OH
30C, isoDromeQC
0.487
H-Gln-Asp-Glu-Leu-NH2
-
pH 8.0, 30C
1.748
H-Gln-Asp-Glu-Leu-NH2
pH 8.0, 30C
2.3
H-Gln-Asp-Glu-Leu-NH2
30C
0.028
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
0.036
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
0.056
H-Gln-beta-naphthylamide
pH 8.0, 30C
0.15
H-Gln-beta-naphthylamide
30C, isoDromeQC
0.17
H-Gln-beta-naphthylamide
30C
0.053
H-Gln-Gln-OH
-
pH 8.0, 30C
0.115
H-Gln-Gln-OH
-
pH 8.0, 30C
0.487
H-Gln-Gln-OH
pH 8.0, 30C
0.94
H-Gln-Gln-OH
30C, isoDromeQC
1.5
H-Gln-Gln-OH
30C
0.08
H-Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.42
H-Gln-Glu-Ala-Phe-NH2
30C
0.68
H-Gln-Glu-Ala-Phe-NH2
30C, isoDromeQC
0.742
H-Gln-Glu-Ala-Phe-NH2
pH 8.0, 30C
0.265
H-Gln-Glu-OH
-
pH 8.0, 30C
0.705
H-Gln-Glu-OH
-
pH 8.0, 30C
2.516
H-Gln-Glu-OH
pH 8.0, 30C
29.5
H-Gln-Glu-OH
30C
0.33
H-Gln-Glu-Tyr-Ala-NH2
pH 8.0, 30C
0.065
H-Gln-Glu-Tyr-Phe-NH2
30C
0.086
H-Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.41
H-Gln-Glu-Tyr-Phe-NH2
pH 8.0, 30C
0.424
H-Gln-Gly-OH
-
pH 8.0, 30C
1.1
H-Gln-Gly-OH
-
pH 8.0, 30C
2.4
H-Gln-Gly-OH
30C, isoDromeQC
2.64
H-Gln-Gly-OH
pH 8.0, 30C
7.1
H-Gln-Gly-OH
30C
0.214
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
0.59 - 1
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
1.17
H-Gln-Gly-Pro-OH
30C, isoDromeQC
1.791
H-Gln-Gly-Pro-OH
pH 8.0, 30C
4.1
H-Gln-Gly-Pro-OH
30C
0.06
H-Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.08
H-Gln-Lys-Arg-Leu-NH2
30C
0.448
H-Gln-Lys-Arg-Leu-NH2
pH 8.0, 30C
0.48
H-Gln-NH2
-
pH 8.0, 30C
2.048
H-Gln-NH2
pH 8.0, 30C
0.106
H-Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.44
H-Gln-Phe-Ala-NH2
pH 8.0, 30C
0.128
H-Gln-Phe-Ala-OH
30C, isoDromeQC
0.35
H-Gln-Phe-Ala-OH
30C
0.247
H-Gln-Ser-Tyr-Phe-NH2
pH 8.0, 30C
0.72
H-Gln-Tyr-Ala-OH
30C, isoDromeQC
0.014
H-Gln-Val-Ala-OH
-
pH 8.0, 30C
0.1293
H-Gln-Val-Ala-OH
30C, isoDromeQC
0.14
H-Gln-Val-Ala-OH
30C
0.282
H-Gln-Val-Ala-OH
pH 8.0, 30C
0.028
L-Gln-2-naphthylamide
-
pH 8.0, 30C
0.039
L-Gln-2-naphthylamide
mutant enzyme E45Q, at pH 7.0 at 25C
0.04
L-Gln-2-naphthylamide
pH 8.0, 30C
0.056
L-Gln-2-naphthylamide
pH 8.0, 30C
0.121
L-Gln-2-naphthylamide
wild type enzyme, at pH 7.0 at 25C
0.4
L-Gln-2-naphthylamide
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.5
L-Gln-2-naphthylamide
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0.048
L-Gln-7-amido-4-methylcoumarin
pH 8.0, 30C
0.05
L-Gln-7-amido-4-methylcoumarin
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0.08
L-Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
0.105
L-Gln-7-amido-4-methylcoumarin
pH 8.0, 30C
0.3
L-Gln-7-amido-4-methylcoumarin
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.205
L-Gln-7-amino-4-methylcoumarin
30C, isoDromeQC
0.31
L-Gln-7-amino-4-methylcoumarin
30C
2.7
L-Gln-amide
in 0.1 M MES, pH 7.5, temperature not specified in the publication
2.9
L-Gln-amide
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.41
L-Gln-Gly
pH 8.0, 30C
1.1
L-Gln-Gly
-
pH 8.0, 30C
2.64
L-Gln-Gly
pH 8.0, 30C
3.8
L-Gln-Gly
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.17
L-Gln-Gly-L-Pro
pH 8.0, 30C
0.59 - 1
L-Gln-Gly-L-Pro
-
pH 8.0, 30C
1.791
L-Gln-Gly-L-Pro
pH 8.0, 30C
0.294
L-Gln-L-Ala
-
pH 8.0, 30C
0.4
L-Gln-L-Ala
pH 8.0, 30C
1.838
L-Gln-L-Ala
pH 8.0, 30C
0.32
L-Gln-L-Arg-Gly-L-Ile-NH2
pH 8.0, 30C
0.36
L-Gln-L-Asn-Gly-L-Ile-NH2
pH 8.0, 30C
0.487
L-Gln-L-Asp-L-Glu-L-Leu-NH2
-
pH 8.0, 30C
1.74
L-Gln-L-Asp-L-Glu-L-Leu-NH2
pH 8.0, 30C
0.053
L-Gln-L-Gln
-
pH 8.0, 30C
0.15
L-Gln-L-Gln
pH 8.0, 30C
0.487
L-Gln-L-Gln
pH 8.0, 30C
6.5
L-Gln-L-Gln
in 0.1 M MES, pH 7.5, temperature not specified in the publication
20.4
L-Gln-L-Gln
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.265
L-Gln-L-Glu
-
pH 8.0, 30C
0.8
L-Gln-L-Glu
pH 8.0, 30C
2.4
L-Gln-L-Glu
in 0.1 M MES, pH 7.5, temperature not specified in the publication
2.516
L-Gln-L-Glu
pH 8.0, 30C
4.2
L-Gln-L-Glu
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.08
L-Gln-L-Glu-L-Ala-L-Phe-NH2
-
pH 8.0, 30C
0.742
L-Gln-L-Glu-L-Ala-L-Phe-NH2
pH 8.0, 30C
0.33
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
pH 8.0, 30C
0.086
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
-
pH 8.0, 30C
0.41
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
pH 8.0, 30C
0.06
L-Gln-L-Lys-L-Arg-L-Leu-NH2
-
pH 8.0, 30C
0.448
L-Gln-L-Lys-L-Arg-L-Leu-NH2
pH 8.0, 30C
0.106
L-Gln-L-Phe-L-Ala-NH2
-
pH 8.0, 30C
0.44
L-Gln-L-Phe-L-Ala-NH2
pH 8.0, 30C
0.247
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
pH 8.0, 30C
0.16
L-Gln-L-Thr-Gly-L-Ile-NH2
pH 8.0, 30C
0.072
L-Gln-L-Trp-L-Ala-NH2
pH 8.0, 30C
0.014
L-Gln-L-Val-L-Ala-NH2
-
pH 8.0, 30C
0.272
L-Gln-L-Val-L-Ala-NH2
pH 8.0, 30C
0.48
L-Gln-NH2
-
pH 8.0, 30C
2.048
L-Gln-NH2
pH 8.0, 30C
0.151
L-Gln-tert-butyl ester
-
pH 8.0, 30C
0.294
L-Gln-tert-butyl ester
pH 8.0, 30C
1
L-Gln-tert-butyl ester
in 0.1 M MES, pH 7.5, temperature not specified in the publication
8.2
L-Gln-tert-butyl ester
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.379
L-glutamine tert-butyl ester
-
pH 6.5
0.4
L-glutamine tert-butyl ester
-
pH 6.75
0.43
L-glutamine tert-butyl ester
-
pH 7.45
0.44
L-glutamine tert-butyl ester
-
pH 8.05
0.46
L-glutamine tert-butyl ester
-
pH 7.0 or pH 8.6
0.55
L-glutamine tert-butyl ester
-
pH 6.2
0.6
L-glutamine tert-butyl ester
-
pH 9.0
0.62
L-glutamine tert-butyl ester
-
pH 6.3
0.9
L-glutamine tert-butyl ester
-
pH 9.55
1.3
L-glutamine tert-butyl ester
-
pH 9.95
0.06
L-glutaminyl-2-naphthylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.051
L-glutaminyl-4-methylcoumarinylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.03
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.03
L-glutaminyl-7-amido-4-methylcoumarin
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.032
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.032
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
0.063
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
1.1
QAAE
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
1.6
QAAE
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.1
QAAF
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.8
QAAF
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.4
QAAR
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.2
QAEA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.7
QAEA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
2.1
QAFA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
2.5
QAFA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.1
QARA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
24.4
QARA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
1
QEAA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
1.1
QEAA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.16
QEDL
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.16
QEDL
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.029
QEYF
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.04
QEYF
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
1.9
QFAA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
3.5
QFAA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.1
QGGG
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
5.6
QGGG
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.9
QRAA
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
2.9
QRAA
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.088
[Gln1,Gly4]-thyrotropin releasing hormone
-
-
-
0.087
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
0.151
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
0.031
[Gln1]-gastrin
-
pH 8.0, 30C
-
0.033
[Gln1]-gastrin
-
pH 8.0, 30C
-
0.053
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
0.054
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
0.023
[Gln1]-neurotensin
-
pH 8.0, 30C
-
0.037
[Gln1]-neurotensin
-
pH 8.0, 30C
-
0.09
[Gln1]-thyrotropin releasing-hormone
-
pH 8.0, 30C
-
0.035
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00022
EFRH-NH2
Homo sapiens
-
at pH 6.5, 150000fold lower compared to Gln substrate
0.00042
EFRHHDSGYE-NH2
Homo sapiens
-
-
41.4
Gln(3)-amyloid-beta peptide 3-11 amide
Homo sapiens
-
pH 6.0, 30C, 1% DMSO
-
55
Gln(3)-amyloid-beta peptide 3-11 amide
Homo sapiens
-
pH 6.0, 30C, in absence of DMSO
-
62
Gln(3)-amyloid-beta peptide 3-21 amide
Homo sapiens
-
pH 6.0, 30C, 1% DMSO
-
40
Gln(3)-amyloid-beta peptide 3-40 amide
Homo sapiens
-
pH 6.0, 30C, 1% DMSO
-
0.0019
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme D305E
0.0042
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme D248Q
0.03
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme D305N
0.068
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme D305A
0.085
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme D248A
0.84
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme S160G
1
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme E201D; pH 8.0, 25C, mutant enzyme H319L
5.3
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, mutant enzyme S160A
5.4
Gln-2-naphthylamide
Homo sapiens
-
pH 8.0, 25C, wild-type enzyme
5.3
Gln-7-amido-4-methylcoumarin
Homo sapiens
-
pH 8.0, 30C
39.4
Gln-7-amino-4-methylcoumarin
Carica papaya
-
pH 8.0, 30C
1.27
Gln-Ala
Homo sapiens
-
-
47.6
Gln-Ala
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
57.2
Gln-Ala
Homo sapiens
-
pH 8.0, 30C
68.1
Gln-Ala
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
69.8
Gln-Ala
Carica papaya
-
pH 8.0, 30C
60.5
Gln-Ala-Ala-Ala-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
74.6
Gln-Ala-Ala-Ala-Ala-NH2
Carica papaya
-
pH 8.0, 30C
76.3
Gln-Ala-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
83.2
Gln-Ala-Ala-NH2
Carica papaya
-
pH 8.0, 30C
55.3
Gln-Ala-Ala-Ser-Ala-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
78.5
Gln-Ala-Ala-Ser-Ala-Ala-NH2
Carica papaya
-
pH 8.0, 30C
29.8
Gln-Arg-Gly-Ile-NH2
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
33.5
Gln-Arg-Gly-Ile-NH2
Homo sapiens
-
pH 8.0, 30C
48.4
Gln-Arg-Gly-Ile-NH2
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
49.2
Gln-Arg-Gly-Ile-NH2
Carica papaya
-
pH 8.0, 30C
29.6
Gln-Arg-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
48.9
Gln-Arg-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
51.4
Gln-Asn-Gly-Ile-NH2
Carica papaya
-
pH 8.0, 30C
56.6
Gln-Asn-Gly-Ile-NH2
Homo sapiens
-
pH 8.0, 30C
20.6
Gln-beta-naphthylamide
Homo sapiens
-
pH 8.0, 30C
51.4
Gln-beta-naphthylamine
Carica papaya
-
pH 8.0, 30C
0.43
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme W207L
0.848
Gln-Gln
Homo sapiens
-
-
0.87
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme E201D
1.35
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme W329A
2.32
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme W207F
2.7
Gln-Gln
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
7.3
Gln-Gln
Homo sapiens
-
pH 7.0, wild-type enzyme
7.35
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme R54W
7.91
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme F146A
8.56
Gln-Gln
Homo sapiens
-
pH 8.8, wild-type enzyme
8.6
Gln-Gln
Homo sapiens
-
-
8.63
Gln-Gln
Homo sapiens
-
pH 8.0, wild-type enzyme
8.66
Gln-Gln
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
9.39
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme Q304L
9.76
Gln-Gln
Homo sapiens
-
pH 7.5, wild-type enzyme
9.93
Gln-Gln
Homo sapiens
-
pH 8.5, wild-type enzyme
10
Gln-Gln
Homo sapiens
-
-
11.67
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme K144A
12.91
Gln-Gln
Homo sapiens
-
pH 8.0, mutant enzyme F325A
20.7
Gln-Gln
Homo sapiens
-
pH 8.0, 30C
43.2
Gln-Gln
Carica papaya
-
pH 8.0, 30C
0.755
Gln-Gln-Gln
Homo sapiens
-
-
12.8
Gln-Gln-OH
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
41.4
Gln-Gln-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
52.9
Gln-Gln-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
2.6
Gln-Glu
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
7.79
Gln-Glu
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
18.9
Gln-Glu
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
24.7
Gln-Glu
Homo sapiens
-
pH 8.0, 30C
44.4
Gln-Glu
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
50.3
Gln-Glu
Carica papaya
-
pH 8.0, 30C
46
Gln-Glu-Ala-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
53.7
Gln-Glu-Ala-Ala-NH2
Carica papaya
-
pH 8.0, 30C
39
Gln-Glu-Ala-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
48.5
Gln-Glu-Ala-Phe-NH2
Carica papaya
-
pH 8.0, 30C
45.6
Gln-Glu-Asp-Leu-NH2
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
45.8
Gln-Glu-Asp-Leu-NH2
Homo sapiens
-
pH 8.0, 30C
53.6
Gln-Glu-Asp-Leu-NH2
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
58.5
Gln-Glu-Asp-Leu-NH2
Carica papaya
-
pH 8.0, 30C
42.1
Gln-Glu-Tyr-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
44.7
Gln-Glu-Tyr-Ala-NH2
Carica papaya
-
pH 8.0, 30C
45.1
Gln-Glu-Tyr-NH2
Homo sapiens
-
pH 8.0, 30C
53.6
Gln-Glu-Tyr-NH2
Carica papaya
-
pH 8.0, 30C
46
Gln-Glu-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
54.6
Gln-Glu-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
0.368
Gln-Gly
Homo sapiens
-
-
1.65
Gln-Gly
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
4.57
Gln-Gly
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
13.2
Gln-Gly
Homo sapiens
-
pH 8.0, 30C
45.8
Gln-Gly
Carica papaya
-
pH 8.0, 30C
4
Gln-Gly-Pro
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
11.4
Gln-Gly-Pro
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
25.3
Gln-Gly-Pro
Homo sapiens
-
pH 8.0, 30C
41.7
Gln-Gly-Pro
Carica papaya
-
pH 8.0, 30C
83
Gln-His-Pro-NH2
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
55.4
Gln-His-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
75.9
Gln-His-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
31.6
Gln-Lys-Arg-Leu-NH2
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
33.4
Gln-Lys-Arg-Leu-NH2
Homo sapiens
-
pH 8.0, 30C
48.2
Gln-Lys-Arg-Leu-NH2
Carica papaya
-
pH 8.0, 30C
58.1
Gln-Lys-Arg-Leu-NH2
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
0.36
Gln-NH2
Homo sapiens
-
mutant enzyme H319Q
0.388
Gln-NH2
Homo sapiens
-
mutant enzyme H307Q
0.435
Gln-NH2
Homo sapiens
-
unmutated enzyme
0.55
Gln-NH2
Homo sapiens
-
-
12.2
Gln-NH2
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
12.8
Gln-NH2
Homo sapiens
-
pH 8.0, 30C
13
Gln-NH2
Homo sapiens
-
-
20.7
Gln-NH2
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
44.8
Gln-NH2
Carica papaya
-
pH 8.0, 30C
45.2
Gln-NH2
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
7.5
Gln-Phe-Ala
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
24.1
Gln-Phe-Ala
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
109
Gln-Phe-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
132
Gln-Phe-Ala-NH2
Carica papaya
-
pH 8.0, 30C
18.8
Gln-Pro-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
31.7
Gln-Pro-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
52.8
Gln-Ser-Tyr-Phe-NH2
Homo sapiens
-
pH 8.0, 30C
64.9
Gln-Ser-Tyr-Phe-NH2
Carica papaya
-
pH 8.0, 30C
6.7
Gln-tert-butyl ester
Homo sapiens
-
pH 8.0, 30C
16
Gln-tert-butyl ester
Homo sapiens
-
-
20.9
Gln-tert-butyl ester
Homo sapiens
-
-
49.4
Gln-tert-butyl ester
Carica papaya
-
pH 8.0, 30C
47
Gln-Trp-Ala-NH2
Homo sapiens
-
pH 8.0, 30C
50
Gln-Trp-Ala-NH2
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
133
Gln-Trp-Ala-NH2
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
151.8
Gln-Trp-Ala-NH2
Carica papaya
-
pH 8.0, 30C
94
Gln-Tyr
Homo sapiens
-
pH 8.0, 30C
7.7
Gln-Tyr-Ala
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
22.9
Gln-Tyr-Ala
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
104
Gln-Tyr-Ala
Carica papaya
-
pH 8.0, 30C
125
Gln-Tyr-Ala
Homo sapiens
-
pH 8.0, 30C
125
Gln-Tyr-Ala-OH
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
220
Gln-Tyr-Ala-OH
Homo sapiens
-
37C, recombinant enzyme, expressed in Pichia pastoris
17.2
Gln-Val
Homo sapiens
-
pH 8.0, 30C
10
glucagon(3-29)
Homo sapiens
-
pH 6.0, 30C, 1% DMSO
-
1.07
H-Gln-7-amino-4-methylcoumarin
Homo sapiens
-
pH 8.0, 30C
19
H-Gln-7-amino-4-methylcoumarin
Arabidopsis thaliana
-
pH 8.0, 30C
25
H-Gln-7-amino-4-methylcoumarin
Solanum tuberosum
Q84WV9
pH 8.0, 30C
16
H-Gln-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
17
H-Gln-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C
30
H-Gln-Ala-OH
Arabidopsis thaliana
-
pH 8.0, 30C
37.7
H-Gln-Ala-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
13.1
H-Gln-Asp-Glu-Leu-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
14
H-Gln-Asp-Glu-Leu-NH2
Drosophila melanogaster
Q9VRQ9
30C
40
H-Gln-Asp-Glu-Leu-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
1.1
H-Gln-beta-naphthylamide
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
1.3
H-Gln-beta-naphthylamide
Drosophila melanogaster
Q9VRQ9
30C
3.4
H-Gln-beta-naphthylamide
Homo sapiens
-
pH 8.0, 30C
8
H-Gln-beta-naphthylamide
Arabidopsis thaliana
-
pH 8.0, 30C
45
H-Gln-beta-naphthylamide
Solanum tuberosum
Q84WV9
pH 8.0, 30C
2.72
H-Gln-Gln-OH
Homo sapiens
-
pH 8.0, 30C
7
H-Gln-Gln-OH
Drosophila melanogaster
Q9VRQ9
30C
7.4
H-Gln-Gln-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
17
H-Gln-Gln-OH
Arabidopsis thaliana
-
pH 8.0, 30C
24
H-Gln-Gln-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
11
H-Gln-Glu-Ala-Phe-NH2
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
12.7
H-Gln-Glu-Ala-Phe-NH2
Drosophila melanogaster
Q9VRQ9
30C
14.9
H-Gln-Glu-Ala-Phe-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
64
H-Gln-Glu-Ala-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
2.65
H-Gln-Glu-OH
Homo sapiens
-
pH 8.0, 30C
16.4
H-Gln-Glu-OH
Arabidopsis thaliana
-
pH 8.0, 30C
30
H-Gln-Glu-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
38
H-Gln-Glu-OH
Drosophila melanogaster
Q9VRQ9
30C
49
H-Gln-Glu-Tyr-Ala-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
6.9
H-Gln-Glu-Tyr-Phe-NH2
Drosophila melanogaster
Q9VRQ9
30C
9.2
H-Gln-Glu-Tyr-Phe-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
50
H-Gln-Glu-Tyr-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
1.66
H-Gln-Gly-OH
Homo sapiens
-
pH 8.0, 30C
2 - 8
H-Gln-Gly-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
4.9
H-Gln-Gly-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
9
H-Gln-Gly-OH
Drosophila melanogaster
Q9VRQ9
30C
22
H-Gln-Gly-OH
Arabidopsis thaliana
-
pH 8.0, 30C
4.02
H-Gln-Gly-Pro-OH
Homo sapiens
-
pH 8.0, 30C
14
H-Gln-Gly-Pro-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
15.7
H-Gln-Gly-Pro-OH
Arabidopsis thaliana
-
pH 8.0, 30C
16
H-Gln-Gly-Pro-OH
Drosophila melanogaster
Q9VRQ9
30C
32
H-Gln-Gly-Pro-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
7
H-Gln-Lys-Arg-Leu-NH2
Drosophila melanogaster
Q9VRQ9
30C
20.6
H-Gln-Lys-Arg-Leu-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
62
H-Gln-Lys-Arg-Leu-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
25
H-Gln-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
37
H-Gln-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
55
H-Gln-Phe-Ala-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
57
H-Gln-Phe-Ala-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
16
H-Gln-Phe-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
22
H-Gln-Phe-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C
44
H-Gln-Ser-Tyr-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
17.04
H-Gln-Tyr-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
3.02
H-Gln-Val-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
5.3
H-Gln-Val-Ala-OH
Drosophila melanogaster
Q9VRQ9
30C
10.9
H-Gln-Val-Ala-OH
Arabidopsis thaliana
-
pH 8.0, 30C
27
H-Gln-Val-Ala-OH
Solanum tuberosum
Q84WV9
pH 8.0, 30C
1.4
L-Gln-2-naphthylamide
Xanthomonas campestris
Q8P8M4
wild type enzyme, at pH 7.0 at 25C
2.7
L-Gln-2-naphthylamide
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
5.8
L-Gln-2-naphthylamide
Xanthomonas campestris
Q8P8M4
mutant enzyme E45Q, at pH 7.0 at 25C
8
L-Gln-2-naphthylamide
Arabidopsis thaliana
-
pH 8.0, 30C
18.9
L-Gln-2-naphthylamide
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
22
L-Gln-2-naphthylamide
Mus musculus
Q9CYK2
pH 8.0, 30C
45
L-Gln-2-naphthylamide
Solanum tuberosum
Q84WV9
pH 8.0, 30C
0.45
L-Gln-7-amido-4-methylcoumarin
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
6
L-Gln-7-amido-4-methylcoumarin
Mus musculus
Q9CYK2
pH 8.0, 30C
7.1
L-Gln-7-amido-4-methylcoumarin
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19
L-Gln-7-amido-4-methylcoumarin
Arabidopsis thaliana
-
pH 8.0, 30C
25
L-Gln-7-amido-4-methylcoumarin
Solanum tuberosum
Q84WV9
pH 8.0, 30C
2.09
L-Gln-7-amino-4-methylcoumarin
Drosophila melanogaster
Q9VRQ9
30C, isoDromeQC
7
L-Gln-7-amino-4-methylcoumarin
Drosophila melanogaster
Q9VRQ9
30C
35.7
L-Gln-amide
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
61.5
L-Gln-amide
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
2 - 8
L-Gln-Gly
Solanum tuberosum
Q84WV9
pH 8.0, 30C
12.4
L-Gln-Gly
Mus musculus
Q9CYK2
pH 8.0, 30C
22
L-Gln-Gly
Arabidopsis thaliana
-
pH 8.0, 30C
32
L-Gln-Gly
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
15.7
L-Gln-Gly-L-Pro
Arabidopsis thaliana
-
pH 8.0, 30C
21.6
L-Gln-Gly-L-Pro
Mus musculus
Q9CYK2
pH 8.0, 30C
32
L-Gln-Gly-L-Pro
Solanum tuberosum
Q84WV9
pH 8.0, 30C
30
L-Gln-L-Ala
Arabidopsis thaliana
-
pH 8.0, 30C
37.7
L-Gln-L-Ala
Solanum tuberosum
Q84WV9
pH 8.0, 30C
42
L-Gln-L-Ala
Mus musculus
Q9CYK2
pH 8.0, 30C
34
L-Gln-L-Arg-Gly-L-Ile-NH2
Mus musculus
Q9CYK2
pH 8.0, 30C
68
L-Gln-L-Asn-Gly-L-Ile-NH2
Mus musculus
Q9CYK2
pH 8.0, 30C
13.1
L-Gln-L-Asp-L-Glu-L-Leu-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
40
L-Gln-L-Asp-L-Glu-L-Leu-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
0.27
L-Gln-L-Gln
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
17
L-Gln-L-Gln
Arabidopsis thaliana
-
pH 8.0, 30C
24
L-Gln-L-Gln
Solanum tuberosum
Q84WV9
pH 8.0, 30C
31.7
L-Gln-L-Gln
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
32
L-Gln-L-Gln
Mus musculus
Q9CYK2
pH 8.0, 30C
0.021
L-Gln-L-Glu
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
12.8
L-Gln-L-Glu
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
16.4
L-Gln-L-Glu
Arabidopsis thaliana
-
pH 8.0, 30C
29
L-Gln-L-Glu
Mus musculus
Q9CYK2
pH 8.0, 30C
30
L-Gln-L-Glu
Solanum tuberosum
Q84WV9
pH 8.0, 30C
14.9
L-Gln-L-Glu-L-Ala-L-Phe-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
64
L-Gln-L-Glu-L-Ala-L-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
49
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
9.2
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
50
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
20.6
L-Gln-L-Lys-L-Arg-L-Leu-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
62
L-Gln-L-Lys-L-Arg-L-Leu-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
55
L-Gln-L-Phe-L-Ala-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
57
L-Gln-L-Phe-L-Ala-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
44
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
23
L-Gln-L-Thr-Gly-L-Ile-NH2
Mus musculus
Q9CYK2
pH 8.0, 30C
41
L-Gln-L-Trp-L-Ala-NH2
Mus musculus
Q9CYK2
pH 8.0, 30C
10.9
L-Gln-L-Val-L-Ala-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
27
L-Gln-L-Val-L-Ala-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
25
L-Gln-NH2
Arabidopsis thaliana
-
pH 8.0, 30C
37
L-Gln-NH2
Solanum tuberosum
Q84WV9
pH 8.0, 30C
21
L-Gln-tert-butyl ester
Arabidopsis thaliana
-
pH 8.0, 30C
31.3
L-Gln-tert-butyl ester
Solanum tuberosum
Q84WV9
pH 8.0, 30C
64.9
L-Gln-tert-butyl ester
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
128
L-Gln-tert-butyl ester
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
33
L-glutamine tert-butyl ester
Carica papaya
-
-
18.8
L-glutaminyl-2-naphthylamide
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
5.4
L-glutaminyl-4-methylcoumarinylamide
Homo sapiens
-
30C, recombinant enzyme, expressed in Pichia pastoris
1.07
L-glutaminyl-7-amido-4-methylcoumarin
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
6.98
L-glutaminyl-7-amido-4-methylcoumarin
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
3.4
L-glutaminyl-beta-naphthylamide
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
17.48
L-glutaminyl-beta-naphthylamide
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
20
N1-naphthalen-2-yl-L-glutamamide
Homo sapiens
-
pH 8.0, 30C, 0.5 M KCl
47.2
N1-naphthalen-2-yl-L-glutamamide
Carica papaya
-
pH 8.0, 30C, 0.5 M KCl
0.054
QAAE
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
40
QAAE
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.00008
QAAF
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
183
QAAF
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
170
QAAR
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0042
QAEA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
40
QAEA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0064
QAFA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
102
QAFA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0032
QARA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
129
QARA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.007
QEAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
17.9
QEAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
6.4
QEDL
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
6.4
QEDL
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
3.3
QEYF
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
11.78
QEYF
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.084
QFAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
60
QFAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
30
QFRH-NH2
Homo sapiens
-
at pH 6.5
40
QFRH-NH2
Mus musculus
-
at pH 6.5
42
QFRH-NH2
Carica papaya
-
at pH 6.5
56
QFRH-NH2
Solanum tuberosum
-
at pH 6.5
0.012
QGGG
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
1.1
QGGG
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0074
QRAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
66
QRAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
37.7
[Gln1]-fertilization promoting peptide
Carica papaya
-
pH 8.0, 30C
-
69.6
[Gln1]-fertilization promoting peptide
Homo sapiens
-
pH 8.0, 30C
-
31.6
[Gln1]-gastrin
Carica papaya
-
pH 8.0, 30C
-
54.1
[Gln1]-gastrin
Homo sapiens
-
pH 8.0, 30C
-
69.2
[Gln1]-gonadotropin releasing-hormone
Homo sapiens
-
pH 8.0, 30C
-
72.4
[Gln1]-gonadotropin releasing-hormone
Carica papaya
-
pH 8.0, 30C
-
37.7
[Gln1]-neurotensin
Carica papaya
-
pH 8.0, 30C
-
48.8
[Gln1]-neurotensin
Homo sapiens
-
pH 8.0, 30C
-
82.8
[Gln1]-thyrotropin releasing-hormone
Homo sapiens
-
pH 8.0, 30C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
15.9
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169955
20.2
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169955
20.9
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169955
21.1
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169955
21.9
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169955
27.6
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169955
45.3
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169955
49.9
39SrpL21 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169955
35
Acp33A protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169959
36
Acp33A protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169959
36.9
Acp33A protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169959
38
Acp33A protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169959
7.8
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169957
8.3
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169957
26.2
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169957
32
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169957
33.9
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169957
40.9
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169957
69
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169957
73.1
ALDH2 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169957
13.9
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169960
17.5
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169960
25.9
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169960
31.2
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0); isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169960
43.3
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169960
50.7
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169960
59.3
DIM-10 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169960
0.000019
EFRH-NH2
Mus musculus
-
at pH 6.5
8823
0.000037
EFRH-NH2
Homo sapiens
-
at pH 6.5
8823
0.00017
EFRH-NH2
Solanum tuberosum
-
at pH 6.5
8823
0.00074
EFRH-NH2
Carica papaya
-
at pH 6.5
8823
0.000073
EFRHHDSGYE-NH2
Homo sapiens
-
at low substrate concentrations
8824
0.00009
EFRHHDSGYE-NH2
Homo sapiens
-
-
8824
54
Gln-Gln
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
1575
95
Gln-Gln
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
1575
9
Gln-Glu
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
4663
16
Gln-Glu
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
4663
9
Gln-Gly
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
5330
28
Gln-Gly
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
5330
38
Gln-Gly-Pro
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
6773
111
Gln-Gly-Pro
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
6773
117
Gln-Phe-Ala
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
19170
403
Gln-Phe-Ala
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
19170
207
Gln-Tyr-Ala
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
7638
394
Gln-Tyr-Ala
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
7638
6.64
L-Gln-2-naphthylamide
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
3931
12.6
L-Gln-2-naphthylamide
Xanthomonas campestris
Q8P8M4
wild type enzyme, at pH 7.0 at 25C
3931
35
L-Gln-2-naphthylamide
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
3931
166.1
L-Gln-2-naphthylamide
Xanthomonas campestris
Q8P8M4
mutant enzyme E45Q, at pH 7.0 at 25C
3931
1.46
L-Gln-7-amido-4-methylcoumarin
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
5401
142
L-Gln-7-amido-4-methylcoumarin
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
5401
13
L-Gln-amide
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19662
21.2
L-Gln-amide
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19662
8.5
L-Gln-Gly
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
4937
0.013
L-Gln-L-Gln
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
6275
4.8
L-Gln-L-Gln
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
6275
0.0049
L-Gln-L-Glu
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
6276
5.4
L-Gln-L-Glu
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
6276
15.7
L-Gln-tert-butyl ester
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
7709
63.6
L-Gln-tert-butyl ester
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
7709
103
L-glutaminyl-7-amido-4-methylcoumarin
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
12059
311
L-glutaminyl-7-amido-4-methylcoumarin
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
12059
229
L-glutaminyl-beta-naphthylamide
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
8825
554
L-glutaminyl-beta-naphthylamide
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
8825
22
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169958
22.8
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169958
25
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169958
25.5
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169958
26.1
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169958
28.9
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169958
32.3
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169958
33.2
MCCB protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169958
4.2
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169961
4.6
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169961
6
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169961
7.4
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169961
20.1
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169961
20.9
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169961
21.5
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169961
23.8
NPLP4 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169961
0.034
QAAE
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19670
36
QAAE
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19670
0.0008
QAAF
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19665
219
QAAF
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19665
412
QAAR
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
42192
0.02
QAEA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19669
55
QAEA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19669
0.0031
QAFA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19664
41
QAFA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19664
0.0013
QARA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19667
1215
QARA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19667
0.0067
QEAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19668
18
QEAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19668
55
QEDL
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
19172
104
QEDL
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
19172
123
QEYF
Homo sapiens
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
19171
413
QEYF
Mus musculus
-
protein expressed in Pichia pastoris, pH 8.0, 30C
19171
0.24
QFAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19663
31
QFAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19663
98.9
QFRH-NH2
Solanum tuberosum
-
at pH 6.5
14944
100
QFRH-NH2
Homo sapiens
-
at pH 6.5
14944
135
QFRH-NH2
Mus musculus
-
at pH 6.5
14944
145
QFRH-NH2
Carica papaya
-
at pH 6.5
14944
0.0021
QGGG
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19671
9
QGGG
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19671
0.0026
QRAA
Zymomonas mobilis
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
19666
76
QRAA
Myxococcus xanthus
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19666
26.2
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
169956
30.2
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
169956
40.9
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
169956
43.4
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
169956
50.2
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
169956
53.3
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
169956
55
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
169956
88
rho-7 protein
Drosophila melanogaster
Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
169956
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.253
(3,4-dichlorophenyl)-2-cyano-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
0.62
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
1.36
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(3,4-dimethoxyphenyl)guanidine
-
-
6.72
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-(trifluoromethyl)phenyl)guanidine
-
-
0.83
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-isopropylphenyl)guanidine
-
-
0.7
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-methoxyphenyl)guanidine
-
-
1.37
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(cyclopropylmethyl)guanidine
-
-
1.53
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-methylguanidine
-
-
1.02
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-phenylguanidine
-
-
1.09
(3-(1H-imidazol-1-yl)propyl)-3-(4-bromophenyl)-2-cyanoguanidine
-
-
1.65
(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
0.13
(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
0.295
1,4-bis-(imidazol-1-yl)methyl-2,5-dimethylbenzene
-
30C, pH 8.0
0.00112
1-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00056
1-(2,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.184
1-(2-oxo-2-phenylethyl)-imidazole
-
30C, pH 8.0
0.00155
1-(3,4-dimethoxybenzyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00233
1-(3,4-dimethoxyphenyl)-3-(2-[1-(1H-imidazol-1-yl)cyclopropyl]ethyl)thiourea
-
pH 8.0, 30C
0.06
1-(3,4-dimethoxyphenyl)-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
0.0063
1-(3,4-dimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
0.00034
1-(3,4-dimethoxyphenyl)-3-[(3R)-3-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
0.00076
1-(3,4-dimethoxyphenyl)-3-[(3S)-3-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
0.00006
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00049
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]urea
-
pH 8.0, 30C
0.00055
1-(3,4-dimethoxyphenyl)-3-[4-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
0.041
1-(3,4-dimethoxyphenyl)-N-(3-(4-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
0.0026
1-(3,4-dimethoxyphenyl)-N-(3-(5-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
0.00075
1-(3,5-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.41
1-(3-aminopropyl)-imidazole
-
30C, pH 8.0
0.0018
1-(4-acetylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00089
1-(4-ethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.0028
1-(4-ethylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.0062
1-(6-phenoxyhexyl)-1H-imidazole
-
-
0.000262
1-Benzylimidazole
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.000607
1-Benzylimidazole
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.0071
1-Benzylimidazole
-
30C, pH 8.0
0.013
1-Benzylimidazole
pH 7
0.019
1-Benzylimidazole
pH 8, isoDromeQC
0.048
1-Benzylimidazole
pH 8
0.114
1-Benzylimidazole
pH 7, isoDromeQC
0.12
1-methyl-5-(beta-aminoethyl)-imidazole
-
30C, pH 8.0
0.000101
1-Methylimidazole
pH 7, isoDromeQC
0.03
1-Methylimidazole
-
30C, pH 8.0
0.036
1-Methylimidazole
pH 7
0.103
1-Methylimidazole
pH 8, isoDromeQC
0.109
1-Methylimidazole
pH 8
0.049
1-vinylimidazole
-
30C, pH 8.0
0.0028
1-[3-(1H-imidazol-1-yl)propyl]-3-(1-naphthyl)thiourea
-
pH 8.0, 30C
0.00034
1-[3-(1H-imidazol-1-yl)propyl]-3-(3,4,5-trimethoxyphenyl)thiourea
-
pH 8.0, 30C
0.00186
1-[3-(1H-imidazol-1-yl)propyl]-3-(3-methoxyphenyl)thiourea
-
pH 8.0, 30C
0.0007
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methoxyphenyl)thiourea
-
pH 8.0, 30C
0.00214
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methylphenyl)thiourea
-
pH 8.0, 30C
0.00166
1-[3-(1H-imidazol-1-yl)propyl]-3-[4-(methylthio)phenyl]thiourea
-
pH 8.0, 30C
0.00097
1-[4-(benzyloxy)phenyl]-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.23
2,3-dihydro-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
0.018
2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
0.083
2,3-dihydro-6-methyl-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-5-phenyl-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
1.8
2-aminobenzimidazole
-
30C, pH 8.0
0.061
2-cyano(3,4,5-trimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
0.082
2-cyano(3-(5-methyl-1H-imidazol-1-yl)propyl)-3-(3,4-dimethylphenyl)guanidine
-
-
0.065
2-cyano-1-[3-(5-methyl-1H-imidazol-1-yl)propyl]-4-phenylbenzene-1-guanidine
-
-
0.58
2-ethyl-4-methyl-imidazole
-
30C, pH 8.0
2.58
2-mercaptoethanol
-
0.165
2-methyl-benzylimidazole
-
30C, pH 8.0
0.3
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-2-thioxoquinazolin-4(1H)-one
-
-
0.36
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-7-methyl-2-thioxothieno[3,2-d]pyrimidin-4(1H)-one
-
-
0.62
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-1,2,3,5,6,7-hexahydro-4H-cyclopenta[4,5]thieno[2,3-d]pyrimidin-4-one
-
-
0.9
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]thieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.34
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.818
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
30C, pH 8.0
0.039
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]-thieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.017
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.0023
4-(2-imidazol-1-yl-ethoxy)-benzoic acid
-
30C, pH 8.0
7.6
4-imidazole-carboxaldehyde
-
30C, pH 8.0
0.073
4-methylimidazole
pH 8
15.5
5-amino-3H-imidazole-4-carboxylic acid amide
-
30C, pH 8.0
0.129
5-hydroxymethyl-4-methyl-imidazole
-
30C, pH 8.0
0.02
6-benzyl-2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.017
benzimidazole
pH 7, isoDromeQC
0.124
benzimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.13
benzimidazole
-
pH 8.0, 30C
0.138
benzimidazole
-
30C, pH 8.0
0.192
benzimidazole
pH 8.0, 30C
0.199
benzimidazole
-
pH 8.0, 30C
0.25
benzimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.549
benzimidazole
pH 7
1.145
benzimidazole
pH 8, isoDromeQC
2.188
benzimidazole
pH 8
0.0035
benzylimidazole
-
pH 8.0, 30C
0.0039
benzylimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.0062
benzylimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.0064
benzylimidazole
pH 8.0, 30C
0.0073
benzylimidazole
-
pH 8.0, 30C
1.824
cacodylate
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
6.696
cacodylate
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.042
cysteamine
pH 8.0, 30C
0.056
cysteamine
pH 8
0.068
cysteamine
-
pH 8.0, 30C
0.069
cysteamine
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.154
cysteamine
pH 7, isoDromeQC
0.205
cysteamine
pH 8, isoDromeQC
0.301
cysteamine
pH 7
3.25
EFRH-NH2
-
at pH 8.0
8.33
EFRH-NH2
-
at pH 8.0
24.64
EFRH-NH2
-
at pH 6.0
35.75
EFRH-NH2
-
at pH 6.0
4.69
EFRHHDSGYE-NH2
-
at pH 8.0
6.1
EFRHHDSGYE-NH2
-
at pH 8.0
8.32
EFRHHDSGYE-NH2
-
at pH 6.0
10.4
EFRHHDSGYE-NH2
-
at pH 6.0
19.3
ethanolamine
-
22
ethylenediamine
-
0.97
Gln-tert-butyl ester
-
pH 8.0, 30C, 0.5 M KCl
1.2
Gln-tert-butyl ester
-
pH 8.0, 30C
1.21
Gln-tert-butyl ester
-
pH 8.0, 30C
1.33
Gln-tert-butyl ester
-
pH 8.0, 30C, 0.5 M KCl
4.5
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
5.73
H-Gln-7-amino-4-methylcoumarin
-
-
1.3
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
1.47
H-Gln-beta-naphthylamide
-
-
0.6
H-His-Trp-OH
-
30C, pH 8.0
14.5
imidazol-4-carbonic acid methylester
-
30C, pH 8.0
0.103
imidazole
-
30C, pH 8.0
0.103
imidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.116
imidazole
-
pH 8.0, 30C
0.16
imidazole
pH 8.0, 30C
0.219
imidazole
-
pH 8.0, 30C
0.235
imidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.304
imidazole
pH 7
0.364
imidazole
pH 8, isoDromeQC
0.671
imidazole
pH 7, isoDromeQC
1.141
imidazole
pH 8
1.54
L-Gln-2-naphthylamide
wild type enzyme, at pH 7.0 at 25C
5.06
L-Gln-2-naphthylamide
mutant enzyme E45Q, at pH 7.0 at 25C
4.47
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
1.57
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
3.55
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.85
L-histamine
-
30C, pH 8.0
0.56
L-histidinamide
-
30C, pH 8.0
4.4
L-histidine
-
30C, pH 8.0
1.53
L-Histidinol
-
30C, pH 8.0
0.023
methylimidazole
pH 8.0, 30C
0.052
methylimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.079
methylimidazole
-
pH 8.0, 30C
0.082
methylimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.027
N,N-dimethylcysteamine
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.051
N-(-1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chloro-benzenamine
-
-
0.54
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.61
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-3,4-dimethoxy-benzenamine
-
-
0.54
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)-benzenamine
-
-
0.57
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chlorobenzenamine
-
-
1.03
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-methoxybenzenamine
-
-
1.17
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)benzenamine
-
-
0.52
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
1.29
N-(1-(3-(4-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.067
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.034
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)benzenamine
-
-
0.044
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)cyclohexanamine
-
-
0.042
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
0.5
N-(3-(1H-imidazol-1-yl)propyl)-N-cyclohexyl-2-nitroethene-1,1-diamine
-
-
20.55
N-(3-(1H-imidazol-1-yl)propyl)-N-methyl-2-nitroethene-1,1-diamine
-
-
0.0012
N-(4-chlorophenyl)-N'-[2-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
30C, pH 8.0
0.167
N-(trimethylsilyl)-imidazole
-
30C, pH 8.0
0.107
N-Acetylimidazole
-
30C, pH 8.0
0.174
N-benzoylimidazole
-
30C, pH 8.0
0.0109
N-diethylcysteamine
-
0.093
N-diethylcysteamine
pH 8
0.405
N-diethylcysteamine
pH 8, isoDromeQC
0.022
N-dimethylcysteamine
-
pH 8.0, 30C
0.029
N-dimethylcysteamine
-
0.063
N-dimethylcysteamine
pH 8, isoDromeQC
0.079
N-dimethylcysteamine
pH 8
0.08
N-dimethylcysteamine
pH 7
0.013
N-methylimidazole
-
pH 8.0, 30C
0.017
N-omega-acetylhistamine
-
30C, pH 8.0
0.0391
N-omega-acetylhistamine
pH 7
0.084
N-omega-acetylhistamine
pH 8
0.14
N-omega-acetylhistamine
pH 8, isoDromeQC
0.827
N-omega-acetylhistamine
pH 7, isoDromeQC
0.002
N-[3-(1H-imidazol-1-yl)propyl]-5-methoxy-1,3-benzothiazol-2-amine
-
pH 8.0, 30C
0.00157
N-[3-(1H-imidazol-1-yl)propyl]-6-methoxy-1,3-benzothiazol-2-amine
-
pH 8.0, 30C
0.001698
Nomega-acetylhistamine
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.005748
Nomega-acetylhistamine
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.078
oxalic acid diimidazolidide
-
30C, pH 8.0
0.000097
P150/03
pH 8, isoDromeQC
-
0.00094
P150/03
pH 7
-
0.00314
P150/03
pH 8
-
0.145
P150/03
pH 7, isoDromeQC
-
0.000095
PBD150
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
-
0.0001
PBD150
-
pH 8.0, 30C
-
0.0001
PBD150
-
wild type enzyme, in 50 mM Tris, pH 8.0, at 30C
-
0.0001013
PBD150
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
-
0.0001093
PBD150
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
-
0.000114
PBD150
-
wild type enzyme, in 50 mM Tris, pH 8.0, at 30C
-
0.0003706
PBD150
isoform isoDromeQC, at 30C in 50 mM MES (pH 6.0)
-
0.000944
PBD150
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
-
0.001817
PBD150
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
-
0.00248
PBD150
isoform DromeQC, at 30C in 50 mM MES (pH 6.0)
-
0.003139
PBD150
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
11.2
pH 8.0, 30C, recombinant enzyme
18.7
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
-
cyclization of glutamic acid
6.8
-
EFRH-NH2 as substrate
7.2 - 7.5
-
-
7.3
-
pituitary enzyme
7.5 - 7.8
-
Gln-peptide as substrate
7.5 - 8
-
for kcat/KM
7.5 - 8
isoform isoDromeQC
8
-
cyclization of glutamine
8.5
-
enzyme from spleen
8.5
-
enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/L NaCl
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 8.3
-
pH 6.5: about 50% of maximal activity, pH 8.3: about 40% of maximal activity, pituitary enzyme
7.5 - 9.3
-
pH 7.5: about 55% of maximal activity, pH 9.3: about 45% of maximal activity, enzyme from spleen
additional information
-
activity changes very slightly in the pH range between 4.5 and 10
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
secretory and non-secretory cell line P3X63Ag8
Manually annotated by BRENDA team
-
high expression of QC in neurons of several cell layers of the cortex and hippocampus
Manually annotated by BRENDA team
-
slightly lower glutaminyl cyclase activity in cerebrospinal fluid of multiple sclerosis patients compared to controls. The presence of glutaminyl cyclase in cerebrospinal fluid can stabilize peptides from degradation by aminopeptidases
Manually annotated by BRENDA team
-
high expression of QC in neurons of several cell layers of the cortex and hippocampus
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
-
plasma cell leukemia cell line
Manually annotated by BRENDA team
-
; estimated as a fraction of papaya latex
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
additional information
-
ubiquitous expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
exclusively localized within the Golgi complex, obviously retained by the N-terminus. Retention in the Golgi complex suggests involvement in the protein maturation machinery
Manually annotated by BRENDA team
-
a 40000 Da enzyme form is exclusively detected in soluble pituitary extract, a 32000 Da enzyme form is detected in soluble fraction of all tissues which show immunoreactivity. The difference in the two immunoreacive proteins lies in the proteolytic cleavage within a hydrophilic region approximately 80 amino acids from the carboxy-terminus
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1)
Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30000
-
fraction are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography is carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry
695532
37520
-
mass spectrometry analysis
660450
48500
-
gel filtration
487992
55000
-
gel filtration
487989
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 32980, native, glycosylated enzyme, SDS-PAGE; x * 33000, native, glycosylated enzyme, SDS-PAGE
?
-
x * 40000, SDS-PAGE; x * 40876, calculation from nucleotide sequence
?
-
x * 38795, deglycosylated enzyme, MALDI-TOF mass analysis
?
-
x * 37000, SDS-PAGE
?
-
x *32000, or x * 34000, SDS-PAGE, different glycosylation forms of recombinant enzyme. x * 29000, SDS-PAGE of deglycosylated enzyme, x * 29381, calculated
?
x *32000, or x * 34000, SDS-PAGE, different glycosylation forms of recombinant enzyme. x * 29000, SDS-PAGE of deglycosylated enzyme, x * 29945, calculated
?
x * 30000, SDS-PAGE; x * 31100, MALDI-TOF mass spectrometry
?
x * 29900, MALDI-TOF mass spectrometry; x * 30000, SDS-PAGE
additional information
-
enzyme contains extensive beta-sheet structure and is likely to have only short immobile loops connecting ist beta-strands
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
treatment of recombinant enzyme with endoglycosidase H results in decrease of mass by 2 kDa. Deglycosylated enzyme has similar kinetic parameters for conversion of Gln-Gln and Gln-7-amido-4-methylcoumarin as native enzyme
glycoprotein
sequence contain potential N-glycosylation sites at Asn53, Asn292, and Asn352
proteolytic modification
sequence contains an ambiguous signal peptide cleavage site leading to a mature enzyme of 345, 340 or 335 amino acids
glycoprotein
sequence contain potential N-glycosylation sites at Asn53, Asn292, and Asn352
proteolytic modification
sequence contains an ambiguous signal peptide cleavage site leading to a mature enzyme of 345, 340 or 335 amino acids
glycoprotein
-
-
glycoprotein
-
presence of terminal mannose residues, mannose and N-acetylglucosamine in a 3:1 molar ratio
glycoprotein
-
-
glycoprotein
-
deglycosylation has no effect on enzymatic activity
glycoprotein
-
N-glycan sugar moiety is bound to Asn49 in the recombinant enzyme. Presence or absence of glycan has no apparent effect on structure, stability, or enzymatic activity of the enzyme
glycoprotein
-
-
glycoprotein
sequence contains a potential glycosylation site at Asp50
glycoprotein
-
-
glycoprotein
-
-
glycoprotein
treatment of recombinant enzyme with endoglycosidase H results in decrease of mass by 2 kDa. Deglycosylated enzyme has similar kinetic parameters for conversion of Gln-Gln and Gln-7-amido-4-methylcoumarin as native enzyme
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters A = 62.82 A, b = 81.23 A, c = 108.17 A and two molecules per asymmetric unit, resolution 1.7 A
-
hatbox-shaped molecule that forms an unusual five-fold beta-propeller traversed by a central channel
-
structure determinantion by single wavelength anomalous diffraction technique using sulfur and zinc as anomalous scatterers. The zinc ion present is bound via an octahedral coordination into an elongated cavity along the pseudo 5fold axis of the beta-propeller fold. The active site is located at the C-terminal entrance of the enzymes central tunnel with residues W83, W110, Q24, E69, N155, K225, F22, and F67 in the entrance
-
hanging drop vapor diffusion method, using 10% (w/v) PEG 35000 and 100 mM Tris (pH 8.5) for isoform DromeQC; hanging drop vapor diffusion method, using 200 mM MnCl2, 30% (w/v) PEG 4000, and 100 mM Tris (pH 8.5) for isoform isoDromeQC
hanging drop vapor diffusion method, using 100 mM imidazole (pH 8.0), 30% (v/v) 2-methyl-2,4-pentanediol, and 11% (w/v) 4000 PEG
-
hanging drop vapor-diffusion method. The space group of the crystal is R32, with typical unit cells of a = b = 119 A and c = 333A, in which an asymmetric unit comprises two human glutaminyl cyclase molecules. Crystal structures of the enzyme bound to inhibitors: 1-vinylimidazole, 1-benzylimidazole and N-omega-acetylhistamine
-
hanging-drop vapor diffusion
-
mutant enzyme W207F, hanging drop vapor diffusion method, using 1.7 M (NH4)2SO4, 4% (v/v) dioxane, and 100 mM MES, pH 6.5, at 25C; sitting drop vapor diffusion method, using either 30% (w/v) PEG 200, 5% (w/v) PEG 3000, and 0.1 M MES, pH 6.0, or 50% (w/v) PEG 200, 0.2 M MgCl2, and 0.1 M cacodylate, pH 6.5
hanging drop vapor diffusion method, using 100 mM sodium acetate (pH 5.3), 200 mM ammonium sulfate, and 12% (w/v) 2000 MME-PEG
-
hanging drop vapor diffusion method, using 0.1 M MES and 30% (w/v) PEG-4000
mutant enzyme E89A, hanging drop vapor diffusion method, using 50 mM imidazole and 0.8 M sodium citrate, pH 8.7, at 25C
hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate and 12% (w/v) PEG-4000
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 9
the enzyme possesses relatively high activities between pH 5.0 and 7.5, but exhibits significantly decreased activities above pH 8.0 and below pH 5.0
720244
4
-
30C, 30 min, about 10% loss of activity
658233
5 - 6
-
30C, 30 min, about 30% loss of activity
658233
5.5
-
30C, 30 min, about 70% loss of activity
658233
5.5 - 9
stable for at least 30 min at 30C, both native and recombinant enzyme. At pH values below or above, rapid inactivation. 0.02 mM ZnSO4 partially prevents denaturation in the acidic range
671982
6
-
30C, 30 min, about 50% loss of activity
658233
6.5
-
30C, 30 min, about 40% loss of activity
658233
6.5 - 8
-
30C, 30 min, about 10% loss of activity
658233
7 - 9
-
30C, 30 min, less than 20% loss of activity
658233
8.5
-
30C, 30 min, about 20% loss of activity
658233
9 - 10
-
30C, 30 min, about 15% loss of activity
658233
9.5
-
30C, 30 min, about 50% loss of activity
658233
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 75
the enzyme is only stable below 40C and exhibits remarkable instability above 50C
720244
65
-
pH 8.8, several hours, no inactivation
487993
85
-
heat stability of QCT up to 85C
695532
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
complete unfolding of the enzyme requires a combination of an acidic medium and chemical denaturant such as urea or guanidine hydrochloride
-
resistance to denaturation induced by guanidine-HCl
-
treatment with trypsin and chymotrypsin at pH 8.2 and 8.0 respectively, for 16 h at 37C: enzyme is recovered in its native form
-
after a 24-h incubation in 1 M guanidine hydrochloride the recombinant enzyme becomes aggregated
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration
-
; Ni-NTA column chromatography and Sephacryl S-100 gel filtration
chelating Sepharose column chromatography and butyl Sepharose column chromatography
-
hanging-drop vapour-diffusion method, wild-type and mutant enzymes
-
His-tagged version
-
of 6His-tagged enzyme expressed in Pichia pastoris and enzyme expressed in Escherichia coli
-
hydrophobic interaction chromatography, ion exchange chromatography, gel filtration
-
recombinant enzyme
Q-Sepharose column chromatography
Ni-NTA column chromatography
Q-Sepharose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
-
expression in Escherichia coli as either His-tagged enzyme with three different signal peptides and in fusions with three different signal peptides and in fusion with thioredoxin, glutathione S-transferase, and (pre-)maltose-binding protein. In all cases, the expressed protein is either undetectable or insoluble. Expression in Pichia pastoris of the enzyme fused to the alpha-factor leader results in low levels of activity. Extracellular expression of the enzyme in the insect cell/baculovirus system is sucessfull. Enzyme N-terminally fused to a combined secretion signal/His-tag peptide is correctly processed by the host signal peptidase and the His-tag can subsequently be removed with dipeptidyl peptidase I
-
expressed in Escherichia coli M15 cells (isoform isoDromeQC); expressed in Pichia pstoris strain X33 (isoform DromeQC)
heterologously expressed in Pichia pastoris and Escherichia coli; heterologously expressed in Pichia pastoris and Escherichia coli
; expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells
cloned into the Escherichia coli expression vectors pMALc2 and pET19b. Expression of this cDNA in either vector results in the production of a glutaminyl cyclase fusion protein which is enzymatically active and reacts with anti-bovine glutaminyl cyclase antisera
-
expressed in HEK-293 cell, expressed in Pichia pastoris
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain X33
-
expression in Drosophila S2 cells
-
expression in Escherichia coli
-
expression in Pichia pastoris. In Escherichia coli only 50% of the protein does not contain a disulfide bond that is present in the enzyme expressed in Pichia pastoris
-
h-isoQC, lacking the N-terminal signal anchor and the short cytosolic tail, is expressed as a fusion protein in Escherichia coli
-
modified N-terminal region, His-tagged version, expressed in Pichia pastoris
-
expressed in Pichia pastoris
-
modified N-terminal region, His-tagged version, expressed in Pichia pastoris
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Pichia pastoris
-
expression in Pichia pastoris
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C113A/C136A
mutant of isoform DromeQC lacking a disulfide bond, without influence on the stability of the enzyme
C136A/C158A
mutant of isoform isoDromeQC lacking a disulfide bond, without influence on the stability of the enzyme
C369A
-
improvement of enzyme secretion (expressed in Pichia pastoris)
D248A
-
no activity
D248A
-
kcat/KM for Gln-2-naphthylamide is 136fold lower than wild-type value
D248Q
-
kcat/KM for Gln-2-naphthylamide is 7123fold lower than wild-type value
D305A
-
kcat/KM for Gln-2-naphthylamide is 1493fold lower than wild-type value
D305E
-
kcat/KM for Gln-2-naphthylamide is 21000fold lower than wild-type value
D305L
-
no activity
D305N
-
kcat/KM for Gln-2-naphthylamide is 772fold lower than wild-type value
E201D
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 200fold lower than wild-type ratio
E201D
-
kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value
E201Q
-
almost no catalytic activity
F146A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.43fold lower than wild-type ratio
F325A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 4.9fold lower than wild-type ratio
F325A
-
the mutant shows a 190fold increase in Ki of PBD150 compared to the wild type enzyme
F325N
-
the mutant shows a 815fold increase in Ki of PBD150 compared to the wild type enzyme
F325Y
-
the mutant shows a 381fold increase in Ki of PBD150 compared to the wild type enzyme
H140Q
-
inactive enzyme
H307Q
-
mutant enzyme with increased KM-value
H319L
-
kcat/KM for Gln-2-naphthylamide is 15fold lower than wild-type value
H319Q
-
mutant enzyme with increased KM-value
H330Q
-
inactive enzyme
I303A
-
the mutation results in a 66.3fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303F
-
the mutation results in a 6.2fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303N
-
the mutation results in a 112fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303V
-
the mutation results in a 1.9fold increase in the Ki value of PBD150 compared to the wild type enzyme
I73N
-
artificial glycosylation site
I73N/C369A
-
further improvement of enzyme secretion (expressed in Pichia pastoris)
K144A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.7fold lower than wild-type ratio
K144A
-
the mutant shows a 1.21fold increase in Ki of PBD150 compared to the wild type enzyme
K144M
-
the mutant shows a 335fold increase in Ki of PBD150 compared to the wild type enzyme
K144R
-
the mutant shows a 237fold increase in Ki of PBD150 compared to the wild type enzyme
N49A
-
mutant enzyme is not glycosylated, kinetic properties are indistinguishable from unmutated enzyme
Q304L
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.7fold lower than wild-type ratio
R54W
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.4fold lower than wild-type ratio
S160A
-
kcat/KM for Gln-2-naphthylamide is 3fold lower than wild-type value
S160G
-
kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value
S323A
-
the mutation leads to a significant decrease (0.2fold) in the Ki value of PBD150 compared to the wild type enzyme
S323T
-
the mutation leads to a significant decrease (0.21fold) in the Ki value of PBD150 compared to the wild type enzyme
S323V
-
the mutation leads to a strong decrease (0.074fold) in the Ki value of PBD150 compared to the wild type enzyme
W207F
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 3.45fold lower than wild-type ratio
W207F
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W207F
the mutation reduces the turnover rate (kcat) of the enzyme by about 3.7fold compared to the wild type enzyme
W207L
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 56fold lower than wild-type ratio
W207L
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W207Q
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W329A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 297fold lower than wild-type ratio
W329F
-
the mutation leads to a significant decrease (0.56fold) in the Ki value of PBD150 compared to the wild type enzyme
W329Y
-
the mutation does not affect the Ki of PBD150 compared to the wild type enzyme
Y299A
-
the mutant shows a 122fold increase in Ki of PBD150 compared to the wild type enzyme
Y299F
-
the mutant shows a 220fold increase in Ki of PBD150 compared to the wild type enzyme
I74N
-
artificial glycosylation site
Q46E
the mutants pH optimum is shifted towards lower pH values, although the activity towards L-glutamine-2-naphtylamine is some 1000fold lower than the wild type
E45A
the mutation leads to a drop in the enzyme activity (1.12% activity compared to the wild type enzyme)
E45Q
the mutation increases the enzyme activity by an order of magnitude (1079.68% activity compared to the wild type enzyme)
E89A
the mutant exhibits 5.46% activity compared to the wild type enzyme
F43A
the mutant exhibits 5.46% activity compared to the wild type enzyme
F87A
the mutant exhibits 3.87% activity compared to the wild type enzyme
W103A
the mutant exhibits 3.05% activity compared to the wild type enzyme
E45A
Xanthomonas campestris ATCC33913
-
the mutation leads to a drop in the enzyme activity (1.12% activity compared to the wild type enzyme)
-
E45Q
Xanthomonas campestris ATCC33913
-
the mutation increases the enzyme activity by an order of magnitude (1079.68% activity compared to the wild type enzyme)
-
E89A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 5.46% activity compared to the wild type enzyme
-
F87A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 3.87% activity compared to the wild type enzyme
-
W103A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 3.05% activity compared to the wild type enzyme
-
E48Q
the mutants pH optimum is shifted towards higher pH values
additional information
-
N53A and N123A: no expression of either of the mutants is detected
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
-
QCT would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease
medicine
-
inhibition of glutaminyl cyclase offers a new therapeutic option for the treatment of Alzheimers disease and provides implications for other amyloidoses, such as familial Danish dementia
medicine
-
the enzyme is a pharmacological target for Alzheimers disease therapy