Information on EC 2.3.2.5 - glutaminyl-peptide cyclotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.3.2.5
-
RECOMMENDED NAME
GeneOntology No.
glutaminyl-peptide cyclotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
general acid/base working mechanism, pivoting around a strictly conserved glutamate
-
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3
show the reaction diagram
DFT theoretical investigation on the catalytic mechanism of glutaminyl cyclase
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
aminoacyl group transfer
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutaminyl-peptide gamma-glutamyltransferase (cyclizing)
Involved in the formation of thyrotropin-releasing hormone and other biologically active peptides containing N-terminal pyroglutamyl residues. The enzyme from papaya also acts on glutaminyl-tRNA.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
cyclotransferase, glutaminyl-transfer ribonucleate
-
-
-
-
DromeQC
Q86PD7
isoform
DromeQC
Q9VRQ9
-
DromeQC
Q9VRQ9
isoform
glutamine cyclotransferase
-, O81226
-
glutaminyl cyclase
-
-
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
Q86PD7, Q9VRQ9
-
glutaminyl cyclase
-
isoenzyme
glutaminyl cyclase
Q16769
-
glutaminyl cyclase
Q9NXS2
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
isoenzyme
glutaminyl cyclase
Q9CYK2
-
glutaminyl cyclase
Q1DDS6
-
glutaminyl cyclase
-
-
glutaminyl cyclase
-
-
glutaminyl cyclase
Q84WV9
-
glutaminyl cyclase
Q8P8M4
-
glutaminyl cyclase
Xanthomonas campestris ATCC33913
Q8P8M4
-
-
glutaminyl cyclase
Q5NLA9
-
glutaminyl-tRNA cyclotransferase
-
-
-
-
Golgi-resident glutaminyl cyclase
Q9NXS2
-
gQC
Q9NXS2
-
h-isoQC
-
-
isoDromeQC
Q86PD7, Q9VRQ9
isoform
QC
-
-
-
-
QCT
-, O81226
-
sQC
-
secretory glutaminyl cyclase
StQC
Q84WV9
-
CAS REGISTRY NUMBER
COMMENTARY
37257-21-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
; expressed in Pichia pastoris
-
-
Manually annotated by BRENDA team
isoform DromeQC
SwissProt
Manually annotated by BRENDA team
isoform isoDromeQC
UniProt
Manually annotated by BRENDA team
expressed in Pichia pastoris
Uniprot
Manually annotated by BRENDA team
neonatal
-
-
Manually annotated by BRENDA team
expressed in Pichia pastoris
-
-
Manually annotated by BRENDA team
Xanthomonas campestris ATCC33913
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
glutaminyl cyclase contributes to the formation of focal and diffuse diglutamate-Abeta peptide deposits in hippocampus
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
39SrpL21 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
Acp33A protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
ALDH2 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
amyloid beta(3-40)
pyroglutamyl-amyloid beta(3-40) + NH3
show the reaction diagram
-
-
-
-
?
amyloid peptide Abeta(3-40,42)
Abeta(3[pE]-40,42) + H2O
show the reaction diagram
-
-
-
-
?
amyloid peptide Abeta(3-40/42)
pGluAbeta(3-40/42) + H2O
show the reaction diagram
-
-
significant portion of Abeta peptides of amyloid plaques in Alzheimer's disease brains
-
?
DIM-10 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
EFRH-NH2
pEFRH-NH2 + H2O
show the reaction diagram
-
-
-
-
?
EFRHHDSGYE-NH2
pEFRHHDSGYE-NH2 + H2O
show the reaction diagram
-
-
-
-
?
Gln
L-5-oxoproline + NH3
show the reaction diagram
-
-
-
-
?
Gln
pyroglutamate + NH3
show the reaction diagram
-
-
-
-
?
Gln(3)-amyloid-beta peptide 3-11 amide
?
show the reaction diagram
-
-
-
-
?
Gln(3)-amyloid-beta peptide 3-21 amide
?
show the reaction diagram
-
-
-
-
?
Gln(3)-amyloid-beta peptide 3-40 amide
?
show the reaction diagram
-
-
-
-
?
Gln-2-naphthylamide
pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln-2-naphthylamide
2-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln-4-methylcoumarinylamide
2-oxoprolyl-4-methylcoumarinylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
-
-
?
Gln-7-amino-4-methylcoumarin
pyroglutamic acid-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
Gln-7-amino-4-methylcoumarin
pyroglutamic acid-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala
Glp-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala
pyroglutamic acid-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala-Ala-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ala-Ala-Ser-Ala-Ala-NH2
pyroglutamic acid-Ala-Ala-Ser-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Arg-Gly-Ile-NH2
pyroglutamic acid-Arg-Gly-Ile-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Arg-Tyr-Phe-NH2
pyroglutamic acid-Arg-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Asn-Gly-Ile-NH2
pyroglutamic acid-Asn-Gly-Ile-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-beta-naphthylamide
pyroglutamic acid-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln-beta-naphthylamide
pyroglutamic acid-beta-naphthylamine + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
L-5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
Glp-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
pyroglutamic acid-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
pyroglutamic acid-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
pyroglutamyl-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
Gln-Gln
5-oxoprolyl-Gln + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
Gln-Gln
pGlu-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln-Gln
Glp-Gln-Gln + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gln-Tyr-Phe-NH2
pyroglutamic acid-Gln-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu
Glp-Glu + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu
Glp-Glu + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu
pyroglutamic acid-Glu + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu
pGlu-Glu + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Ala-Ala-NH2
pyroglutamic acid-Glu-Ala-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Ala-Phe-NH2
pyroglutamic acid-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Asp-Leu-NH2
pyroglutamic acid-Glu-Asp-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Tyr-Ala-NH2
pyroglutamic acid-Glu-Tyr-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Tyr-NH2
pyroglutamic acid-Glu-Tyr-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Glu-Tyr-Phe-NH2
pyroglutamic acid-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly
Glp-Gly + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly
pyroglutamic acid-Gly + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly
pGlu-Gly + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly-Pro
Glp-Gly-Pro + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly-Pro
pyroglutamic acid-Gly-Pro + NH3
show the reaction diagram
-
-
-
-
?
Gln-Gly-Pro
pGlu-Gly-Pro + NH3
show the reaction diagram
-
-
-
-
?
Gln-His-Pro
pyroglutamyl-His-Pro
show the reaction diagram
-
-
-
-
?
Gln-His-Pro-NH2
L-5-oxoprolyl-His-Pro-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-His-Tyr-Phe-NH2
pyroglutamic acid-His-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Lys-Arg-Leu-NH2
pyroglutamic acid-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
-
?
Gln-NH2
Glp-amide + NH3
show the reaction diagram
-
-
-
-
?
Gln-Phe-Ala
pGlu-Phe-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Phe-Ala-NH2
pyroglutamic acid-Phe-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Pro-Tyr-Phe-NH2
pyroglutamic acid-Pro-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Ser-Tyr-Phe-NH2
pyroglutamic acid-Ser-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
-
?
Gln-tert-butyl ester
pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
-
?
Gln-Trp-Ala-NH2
pyroglutamic acid-Trp-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
Gln-Tyr
pyroglutamic acid-Tyr + NH3
show the reaction diagram
-
-
-
-
?
Gln-Tyr-Ala
pyroglutamic acid-Tyr-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Tyr-Ala
pGlu-Tyr-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Tyr-Ala-OH
L-5-oxoprolyl-Tyr-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gln-Val
Glp-Val + NH3
show the reaction diagram
-
-
-
-
?
Gln-Val
pyroglutamic acid-Val + NH3
show the reaction diagram
-
-
-
-
?
Glu-2-naphthylamide
?
show the reaction diagram
-
-
-
-
?
glucagon(3-29)
?
show the reaction diagram
-
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-7-amino-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
? + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-7-amino-4-methylcoumarin
? + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Ala-OH
5-oxoprolyl-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Asp-Glu-Leu-NH2
5-oxoprolyl-Asp-Glu-Leu-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-beta-naphthylamide
5-oxoprolyl-beta-naphthylamide + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Glu-Ala-Phe-NH2
5-oxoprolyl-Glu-Ala-Phe-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Glu-OH
5-oxoprolyl-Glu-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Glu-Tyr-Ala-NH2
5-oxoprolyl-Glu-Tyr-Ala-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Glu-Tyr-Phe-NH2
5-oxoprolyl-Glu-Tyr-Phe-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Gly-OH
5-oxoprolyl-Gly-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Gly-Pro-OH
5-oxoprolyl-Gly-Pro-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Lys-Arg-Leu-NH2
5-oxoprolyl-Lys-Arg-Leu-NH2 + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-NH2
? + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-NH2
? + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Phe-Ala-NH2
5-oxoprolyl-Phe-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Phe-Ala-NH2
5-oxoprolyl-Phe-Ala-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Phe-Ala-OH
5-oxoprolyl-Phe-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Phe-Ala-OH
5-oxoprolyl-Phe-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Phe-Arg-His-NH2
5-oxoprolyl-Phe-Arg-His-NH2 + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Ser-Tyr-Phe-NH2
5-oxoprolyl-Ser-Tyr-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Tyr-Ala-OH
5-oxoprolyl-Tyr-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Tyr-Ala-OH
5-oxoprolyl-Tyr-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-
-
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
H-Gln-Val-Ala-OH
5-oxoprolyl-Val-Ala-OH + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
L-Gln(3)-amyloid-beta peptide 3-42
L-pyroglutamyl(3)-amyloid-beta peptide 3-42 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-2-naphthylamide
L-pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
reaction in cell supernatant is exclusively enzyme-catalyzed
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Q9NXS2
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Q8P8M4, -
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
L-Gln-2-naphthylamide
5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
Xanthomonas campestris ATCC33913
Q8P8M4
-
-
-
?
L-Gln-7-amido-4-methylcoumaride
5-oxoprolyl-7-amido-4-methylcoumaride + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-7-amido-4-methylcoumaride
5-oxoprolyl-7-amido-4-methylcoumaride + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
pyroglutamate-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-7-amido-4-methylcoumarin
5-oxoprolyl-7-amido-4-methylcoumarin + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
L-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-, Q9VRQ9
-
-
-
?
L-Gln-7-amino-4-methylcoumarin
5-oxoprolyl-4-methylcoumarin-7-amide + NH3
show the reaction diagram
-, Q9VRQ9
isoDromeQC
-
-
?
L-Gln-amide
5-oxoprolyl-amide + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-amide
5-oxoprolyl-amide + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-Gly
L-pyroglutamyl-Gly + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-Gly
5-oxoprolyl-Gly + NH3
show the reaction diagram
-
-
-
-
-
L-Gln-Gly
5-oxoprolylglycine + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-Gly
5-oxoprolylglycine + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-Gly-L-Pro
pyroglutamyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-Gly-L-Pro
5-oxoprolyl-Gly-L-Pro + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Ala
L-pyroglutamyl-L-Ala + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Arg-Gly-L-Ile-NH2
pyroglutamyl-L-Arg-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Asn-Gly-L-Ile-NH2
L-pyroglutamyl-L-Asn-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Asp-L-Glu-L-Leu-NH2
pyroglutamyl-L-Asp-L-Glu-L-Leu-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Asp-L-Glu-L-Leu-NH2
pyroglutamyl-L-Asp-L-Glu-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Gln
L-pyroglutamyl-L-Gln + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-L-Gln
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
L-Gln-L-Gln-OH
5-oxoprolyl-L-Gln + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Glu
L-pyroglutamyl-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-L-Glu
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
L-Gln-L-Glu-L-Ala-L-Phe-NH2
pyroglutamyl-L-Glu-L-Ala-L-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Glu-L-Ala-L-Phe-NH2
pyroglutamyl-L-Glu-L-Ala-L-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Glu-L-Asp-L-Leu-NH2
5-oxoprolyl-L-Glu-L-Asp-L-Leu-NH2 + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
pyroglutamic acid-L-Glu-L-Tyr-L-Ala-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
pyroglutamyl-L-Glu-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
pyroglutamyl-L-Glu-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Glu-OH
5-oxoprolyl-L-Glu + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu
5-oxoprolyl-L-Lys-L-Arg-L-Leu + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
pyroglutamyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
pyroglutamyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Lys-L-Arg-L-Leu-NH2
5-oxoprolyl-L-Lys-L-Arg-L-Leu-NH2 + NH3
show the reaction diagram
-
-
-
-
-
L-Gln-L-Phe-L-Ala
5-oxoprolyl-L-Phe-L-Ala + NH3
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
L-pyroglutamyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
L-pyroglutamyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
-
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
-
L-Gln-L-Phe-L-Ala-NH2
5-oxoprolyl-L-Phe-L-Ala-NH2 + NH3
show the reaction diagram
Q9CYK2
-
-
-
?
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
pyroglutamic acid-L-Ser-L-Tyr-L-Phe-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Thr-Gly-L-Ile-NH2
L-pyroglutamyl-L-Thr-Gly-L-Ile-NH2 + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Trp-L-Ala-NH2
L-pyroglutamyl-L-Trp-L-Ala-NH2 + NH3
show the reaction diagram
-, Q9CYK2
-
-
-
?
L-Gln-L-Val-L-Ala-NH2
L-pyroglutamyl-L-Val-L-Ala-NH2 + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-L-Val-L-Ala-NH2
L-pyroglutamyl-L-Val-L-Ala-NH2 + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-NH2
L-pyroglutamyl amide + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-tert-butyl ester
L-pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
Q84WV9, -
-
-
-
?
L-Gln-tert-butyl ester
L-pyroglutamyl-tert-butyl ester + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-tert-butyl ester
5-oxoprolyl-tert-butyl ester
show the reaction diagram
Q5NLA9
-
-
-
?
L-Gln-tert-butyl ester
5-oxoprolyl-tert-butyl ester
show the reaction diagram
Q1DDS6
-
-
-
?
L-Glu(3)-amyloid-beta peptide 3-42
L-pyroglutamyl(3)-amyloid-beta peptide 3-42 + H2O
show the reaction diagram
-
-
-
-
?
L-glutamine tert-butyl ester
L-pyroglutamate tert-butyl ester + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-2-naphthylamide
L-5-oxoprolyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
-
L-glutaminyl-4-methylcoumarinylamide
L-5-oxoprolyl-4-methylcoumarinylamide + NH3
show the reaction diagram
-
-
-
-
-
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-7-amido-4-methylcoumarin
pGlu-7-amido-4-methylcoumarin + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-beta-naphthylamide
pGlu-beta-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
simple intramolecular cyclization. The mechanism consists of the following main steps: 1. intramolecular nucleophilic attack on the gamma-C=O carbon by the nitrogen of the alpha-amino group, 2. transfer of a proton from the alpha-amino group to the nitrogen of the amide group, facilitated by an acidic group of the enzyme, 3. expulsion of the ammonia-leaving group promoted by this or another acidic enzyme group
-
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
thyrotropin-releasing hormone
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
Gln-His-Pro-Glythyrotropin-releasing hormone, gonadotropin-releasing hormone and L-Gln-Tyr-Ala
-
-
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the N-terminal formation of 5-oxoproline is a common post-translational event during biosynthesis of a number of peptides
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the enzyme may participate in the posttranslational processing of hormonal precursors to pyroglutamyl peptides
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
involved in posttranslational modification of the N-terminal glutamine of peptide hormones or neurotransmitters, such as thyrotropin releasing hormone, luteinizing hormone releasing hormone, gastrin, heavy chain of gamma-globulin
-
-
?
NPLP4 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
QAAE
5-oxoprolyl-AAE + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QAAE
5-oxoprolyl-AAE + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QAAF
5-oxoprolyl-AAF + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QAAF
5-oxoprolyl-AAF + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QAAR
5-oxoprolyl-AAR + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QAEA
5-oxoprolyl-AEA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QAEA
5-oxoprolyl-AEA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QAFA
5-oxoprolyl-AFA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QAFA
5-oxoprolyl-AFA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QARA
5-oxoprolyl-ARA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QARA
5-oxoprolyl-ARA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QEAA
5-oxoprolyl-EAA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QEAA
5-oxoprolyl-EAA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QEDL
pEEDL + NH3
show the reaction diagram
-
-
-
-
?
QEYF
pEEYF + NH3
show the reaction diagram
-
-
-
-
?
QFAA
5-oxoprolyl-FAA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QFAA
5-oxoprolyl-FAA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QFRH-NH2
pEFRH-NH2 + H2O
show the reaction diagram
-
-
-
-
?
QFRH-NH2
pEFRH-NH2 + NH3
show the reaction diagram
-
-
-
-
?
QGGG
5-oxoprolyl-GGG + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QGGG
5-oxoprolyl-GGG + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
QRAA
5-oxoprolyl-RAA + NH3
show the reaction diagram
Q5NLA9
-
-
-
?
QRAA
5-oxoprolyl-RAA + NH3
show the reaction diagram
Q1DDS6
-
-
-
?
rho-7 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
[Gln1]-fertilization promoting peptide
[pyroglutamyl]-fertilization promoting peptide + NH3
show the reaction diagram
-
-
-
-
?
[Gln1]-gastrin
[pyroglutamyl]-gastrin + NH3
show the reaction diagram
-
-
-
-
?
[Gln1]-gonadotropin releasing-hormone
[pyroglutamyl]-gonadotropin releasing-hormone + NH3
show the reaction diagram
-
-
-
-
?
[Gln1]-neurotensin
[pyroglutamyl]-neurotensin + NH3
show the reaction diagram
-
-
-
-
?
[Gln1]-thyrotropin releasing-hormone
[pyroglutamyl]-thyrotropin releasing-hormone + NH3
show the reaction diagram
-
-
-
-
?
MCCB protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
additional information
?
-
-
Cys residues do not participate to the catalytic events
-
-
-
additional information
?
-
-
the second amino acid residue in N-terminal glutaminyl peptides significantly accelerates activity while the third residue provides no further rate enhancement. Substrate binding is the main specificity-determining step
-
-
-
additional information
?
-
-
no substrates are D-Gln-Tyr-Ala and Lys-Arg-Gln-His-Pro-Gly-Lys-Arg, i.e. thyrotropin releasing hormone precursor
-
-
-
additional information
?
-
-
the enzyme may play a key role in posttranslational modification
-
-
-
additional information
?
-
-
enzyme is important during cellular maturation of L-pyroglutamyl-containing peptides
-
-
-
additional information
?
-
A7ISW1, -
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
A7ISW2, -
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
Q84WV9, -
highest specificities are observed with substrates possessing large hydrophobic residues adjacent to the N-terminal glutamine and for fluorogenic dipeptide surrogates. Enzyme also catalyzes the conversion of N-terminal glutamic acid residues to pyroglutamic acid, but with about 100000fold lower specificity
-
-
-
additional information
?
-
-
highest specificities are observed with substrates possessing large hydrophobic residues adjacent to the N-terminal glutamine and for fluorogenic dipeptide surrogates. Enzyme also catalyzes the conversion of N-terminal glutamic acid residues to pyroglutamic acid, but with about 100000fold lower specificity
-
-
-
additional information
?
-
-
catalyzes the formation of amyloid-beta3(pE)-40/42 after amyloidogenic processing of amyloid precursor protein
-
-
-
additional information
?
-
Q1DDS6
the enzyme shows no activity towards L-Gln-Gly
-
-
-
additional information
?
-
Q5NLA9
the enzyme shows no activity towards QAAR
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
39SrpL21 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
Acp33A protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
ALDH2 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
DIM-10 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
-
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the N-terminal formation of 5-oxoproline is a common post-translational event during biosynthesis of a number of peptides
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
the enzyme may participate in the posttranslational processing of hormonal precursors to pyroglutamyl peptides
-
-
?
L-glutaminyl-peptide
5-oxoprolyl-peptide + NH3
show the reaction diagram
-
involved in posttranslational modification of the N-terminal glutamine of peptide hormones or neurotransmitters, such as thyrotropin releasing hormone, luteinizing hormone releasing hormone, gastrin, heavy chain of gamma-globulin
-
-
?
NPLP4 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
rho-7 protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
MCCB protein
?
show the reaction diagram
-, Q86PD7, Q9VRQ9
-
-
-
?
additional information
?
-
-
the enzyme may play a key role in posttranslational modification
-
-
-
additional information
?
-
-
enzyme is important during cellular maturation of L-pyroglutamyl-containing peptides
-
-
-
additional information
?
-
A7ISW1, -
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
additional information
?
-
A7ISW2, -
enzyme is involved in N-terminal pyroglutamate formation of snake venom toxins
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
Q8P8M4, -
contains a calcium ion
Co2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be restored partially by Co2+ or Mn2+
Zinc
-
contains one zinc ion per protein molecule
Zinc
-, Q9CYK2
stoichiometric amounts of zinc bound to protein. Depletion of zinc has no significant effect on protein structure, metal has a catalytic role
Zn
-, Q9VRQ9
equimolar protein-bound zinc
Zn2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA
Zn2+
-
the active site is coordinated to three conserved residues and one water molecule, which is replaced by an imidazole nitrogen upon binding of inhibitor
Zn2+
-
-
Zn2+
-
the enzyme contains a catalytic zinc ion
Zn2+
-, Q86PD7, Q9VRQ9
contains zinc; contains zinc
Zn2+
Q9NXS2
the enzyme contains one zinc ion; the enzyme contains one zinc ion
Mn2+
-
enzyme inactivated by 1,10-phenanthroline or dipicolinic acid can be restored partially by Co2+ or Mn2+
additional information
-
substrate specificity for substrates with uncharged backbone and for Gln-Ala and Gln-Glu, does not change significantly by salt addition. With the positively charged substrates Gln-Arg-Gly-Ile-NH2 and Gln-Lys-Arg-Leu-NH2 addition of salt reveals a positive effect on catalysis
additional information
-
substrate specificity for substrates with uncharged backbone does not change significantly by salt addition. For substrates such as Gln-Ala and Gln-Glu, addition of KCl decreases specificity. With the positively charged substrates Gln-Arg-Gly-Ile-NH2 and Gln-Lys-Arg-Leu-NH2 addition of salt reveals a positive effect on catalysis
additional information
-
human glutaminyl cyclase and bacterial zinc aminopeptidase share a common fold and active site. Glutaminyl cyclase does not appear to require zinc for enzymatic activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(3,4-dichlorophenyl)-2-cyano-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(3,4-dimethoxyphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-(trifluoromethyl)phenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-isopropylphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-methoxyphenyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(cyclopropylmethyl)guanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-methylguanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-phenylguanidine
-
-
(3-(1H-imidazol-1-yl)propyl)-3-(4-bromophenyl)-2-cyanoguanidine
-
-
(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
1,10-phenanthroline
-
2 mM, complete inhibition
1,10-phenanthroline
-
inactivated enzyme can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA, little reactivation by Co2+ and Mn2+
1,10-phenanthroline
-, Q9CYK2
time-dependent inactivation
1,10-phenanthroline
-
-
1,4-bis-(imidazol-1-yl)methyl-2,5-dimethylbenzene
-
-
1-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(2,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(2-oxo-2-phenylethyl)-imidazole
-
-
1-(3,4-dimethoxybenzyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(2-[1-(1H-imidazol-1-yl)cyclopropyl]ethyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[(3R)-3-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[(3S)-3-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
i.e. P150/03, complete inhibition at 0.01 mM
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]urea
-
-
1-(3,4-dimethoxyphenyl)-3-[4-(1H-imidazol-1-yl)butyl]thiourea
-
-
1-(3,4-dimethoxyphenyl)-N-(3-(4-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
1-(3,4-dimethoxyphenyl)-N-(3-(5-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
1-(3,5-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(3-aminopropyl)-imidazole
-
-
1-(4-acetylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(4-ethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(4-ethylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
1-(6-phenoxyhexyl)-1H-imidazole
-
-
1-Benzylimidazole
-
0.14 mM, 58% inhibition
1-Benzylimidazole
-, Q9VRQ9
; isoDromeQC
1-Benzylimidazole
-
-
1-Benzylimidazole
Q9NXS2
;
1-methyl-4-(beta-aminoethyl)-imidazole
-
-
1-methyl-5-(beta-aminoethyl)-imidazole
-
-
1-Methylimidazole
-, Q9VRQ9
; isoDromeQC
1-Methylimidazole
-
-
1-Methylimidazole
-
-
1-Methylimidazole
-, Q86PD7, Q9VRQ9
;
1-vinylimidazole
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(1-naphthyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3,4,5-trimethoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3-methoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methoxyphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methylphenyl)thiourea
-
-
1-[3-(1H-imidazol-1-yl)propyl]-3-[4-(methylthio)phenyl]thiourea
-
-
1-[4-(benzyloxy)phenyl]-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
-
2,3-dihydro-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
2,3-dihydro-6-methyl-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-5-phenyl-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
2,6-dipicolinic acid
-, Q9CYK2
time-dependent inactivation
2-aminobenzimidazole
-
-
2-cyano(3,4,5-trimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
2-cyano(3-(5-methyl-1H-imidazol-1-yl)propyl)-3-(3,4-dimethylphenyl)guanidine
-
-
2-cyano-1-[3-(5-methyl-1H-imidazol-1-yl)propyl]-4-phenylbenzene-1-guanidine
-
-
2-ethyl-4-methyl-imidazole
-
-
2-mercaptoethanol
-, Q9CYK2
-
2-methyl-benzylimidazole
-
-
3,5-diamino-1,2-S4-triazole
-
0.14 mM, 10% inhibition
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-2-thioxoquinazolin-4(1H)-one
-
-
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-7-methyl-2-thioxothieno[3,2-d]pyrimidin-4(1H)-one
-
-
3-Amino-1,2,4-triazole
-
0.14 mM, 15% inhibition
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-1,2,3,5,6,7-hexahydro-4H-cyclopenta[4,5]thieno[2,3-d]pyrimidin-4-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]thieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]-thieno[2,3-d]pyrimidin-4(1H)-one
-
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
4-(2-imidazol-1-yl-ethoxy)-benzoic acid
-
-
4-imidazole-carboxaldehyde
-
-
4-methylimidazole
-, Q9VRQ9
-
4-phenyl-1,2,4-triazoleine-3,5-dione
-
0.14 mM, 22% inhibition
5-(methylthio)-1-H-tetrazole
-
0.14 mM, 11% inhibition
5-amino-3H-imidazole-4-carboxylic acid amide
-
-
5-hydroxymethyl-4-methyl-imidazole
-
-
6-benzyl-2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
6-methylpterin
-
-
8-hydroxyquinoline
-, Q9CYK2
time-dependent inactivation
ascorbate
-
-
benzimidazole
-
-
benzimidazole
-, Q9VRQ9
; isoDromeQC
benzimidazole
-
-
benzimidazole
-
competitive
benzimidazole
-, Q86PD7, Q9VRQ9
;
benzylimidazole
-
-
benzylimidazole
-
competitive
benzylimidazole
-
-
benzylimidazole
-
-
benzylimidazole
-, Q86PD7, Q9VRQ9
;
Cacodylate
Q9NXS2
;
Citric acid
-, Q9CYK2
inactivation at pH 5.5, Zn2+ protects
CuCl2
-
0.1 mM
cysteamine
-, Q9CYK2
competitive
cysteamine
-, Q9VRQ9
; isoDromeQC
diethyl dicarbonate
-
rapid inactivation by modification of three essential His residues, at neutral pH, partial reactivation with hydroxylamine
dipicolinic acid
-
inactivated enzyme can be fully restored by addition of Zn2+ in the presence of equimolar concentrations of EDTA, little reactivation by Co2+ and Mn2+
EFRH-NH2
-
competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate
EFRHHDSGYE-NH2
-
competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate
ethanolamine
-, Q9CYK2
-
ethyl-1H-tetrazole-4-acetate
-
0.14 mM, 9% inhibition
ethylamine
-, Q9CYK2
-
ethylenediamine
-, Q9CYK2
-
ethylmercaptane
-, Q9CYK2
-
FADH2
-
2 mM
Gln-tert-butyl ester
-
-
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
Q84WV9, -
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-7-amino-4-methylcoumarin
-
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-Gln-beta-naphthylamide
Q84WV9, -
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-Gln-beta-naphthylamide
-
substrate inhibition
H-His-Trp-OH
-
-
imidazol-4-carbonic acid methylester
-
-
imidazole
-, Q9VRQ9
; isoDromeQC
imidazole
-, Q86PD7, Q9VRQ9
;
L-Gln-2-naphthylamide
Q8P8M4, -
substrate inhibition
L-glutamine-4-nitroanilide
-, Q9VRQ9
substrate inhibition; substrate inhibition, isoDromeQC
L-glutaminyl-7-amido-4-methylcoumarin
-
substrate inhibition
L-glutaminyl-beta-naphthylamide
-
substrate inhibition
L-histamine
-
-
L-histidinamide
-
-
L-histidine
-
-
L-Histidinol
-
-
methylimidazole
-
-
methylimidazole
-
-
N,N-dimethylcysteamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-3,4-dimethoxy-benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)-benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chlorobenzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-methoxybenzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)benzenamine
-
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
N-(1-(3-(4-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)benzenamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chloro-benzenamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)cyclohexanamine
-
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
N-(3-(1H-imidazol-1-yl)propyl)-N-cyclohexyl-2-nitroethene-1,1-diamine
-
-
N-(3-(1H-imidazol-1-yl)propyl)-N-methyl-2-nitroethene-1,1-diamine
-
-
N-(4-chlorophenyl)-N'-[2-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
-
N-(trimethylsilyl)-imidazole
-
-
N-Acetylimidazole
-
-
N-benzoylimidazole
-
-
N-diethylcysteamine
-, Q9CYK2
-
N-diethylcysteamine
-, Q9VRQ9
;
N-dimethylcysteamine
-, Q9CYK2
-
N-dimethylcysteamine
-, Q9VRQ9
; isoDromeQC
N-dimethylcysteamine
-
-
N-methylimidazole
-
-
N-omega-acetylhistamine
-
0.14 mM, 33% inhibition
N-omega-acetylhistamine
-, Q9VRQ9
; isoDromeQC
N-omega-acetylhistamine
-
-
N-[3-(1H-imidazol-1-yl)propyl]-5-methoxy-1,3-benzothiazol-2-amine
-
-
N-[3-(1H-imidazol-1-yl)propyl]-6-methoxy-1,3-benzothiazol-2-amine
-
-
NH4Cl
-
1.0 M
NiCl2
-
0.1 mM
nitron
-
0.14 mM, 39% inhibition
Nomega-acetylhistamine
-
-
Nomega-acetylhistamine
-, Q86PD7, Q9VRQ9
;
Nomega-acetylhistamine
Q9NXS2
;
oxalic acid diimidazolidide
-
-
P150/03
-, Q9VRQ9
; isoDromeQC
-
PBD150
-
-
-
PBD150
-
-
-
PBD150
-
highly potent inhibitor
-
PBD150
-, Q86PD7, Q9VRQ9
potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea; potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea
-
PBD150
-
potent inhibitor
-
PBD150
Q9NXS2
;
-
ZnCl2
-
0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
stimulates
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6
-
EFRH-NH2
-
at pH 6.5, 10times higher compared to KM-value of Gln substrate
4.6
-
EFRHHDSGYE-NH2
-
-
0.087
-
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, in absence of DMSO
-
0.155
-
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, 1% DMSO
-
0.162
-
Gln(3)-amyloid-beta peptide 3-21 amide
-
pH 6.0, 30C, 1% DMSO
-
0.089
-
Gln(3)-amyloid-beta peptide 3-40 amide
-
pH 6.0, 30C, 1% DMSO
-
0.058
-
Gln-2-naphthylamide
-
pH 8.0, 25C, wild-type enzyme
0.125
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248A
0.161
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme H319L
0.174
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160A
0.254
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305N
0.33
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248Q
0.431
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305E
0.615
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160G
0.736
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme E201D
1.1
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305A
0.054
-
Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
0.042
-
Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.143
-
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
0.158
-
Gln-Ala
-
pH 8.0, 30C
0.21
-
Gln-Ala
-
-
0.232
-
Gln-Ala
-
pH 8.0, 30C
0.357
-
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
1.3
-
Gln-Ala
-
-
0.065
-
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
0.197
-
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
0.087
-
Gln-Ala-Ala-NH2
-
pH 8.0, 30Cl
0.164
-
Gln-Ala-Ala-NH2
-
pH 8.0, 30Cl
0.079
-
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
0.216
-
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
0.065
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.091
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.123
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
0.143
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
0.055
-
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
0.124
-
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
0.153
-
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
0.172
-
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
0.07
-
Gln-beta-naphthylamide
-
pH 8.0, 30C
0.038
-
Gln-beta-naphthylamine
-
pH 8.0, 30C
0.044
-
Gln-Gln
-
pH 8.0, 30C
0.09
-
Gln-Gln
-
-
0.092
-
Gln-Gln
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.11
-
Gln-Gln
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.148
-
Gln-Gln
-
pH 8.0, 30C
0.59
-
Gln-Gln
-
pH 8.0, mutant enzyme W207F
0.6
-
Gln-Gln
-
-
0.63
-
Gln-Gln
-
pH 8.0, wild-type enzyme
0.64
-
Gln-Gln
-
-
0.7
-
Gln-Gln
-
-
0.76
-
Gln-Gln
-
pH 8.0, mutant enzyme R54W
0.79
-
Gln-Gln
-
pH 7.0, wild-type enzyme
0.82
-
Gln-Gln
-
pH 8.0, mutant enzyme F146A
0.9
-
Gln-Gln
-
pH 7.5, wild-type enzyme; pH 8.5, wild-type enzyme
1.16
-
Gln-Gln
-
pH 8.0, mutant enzyme Q304L
1.47
-
Gln-Gln
-
pH 8.0, mutant enzyme K144A
1.77
-
Gln-Gln
-
pH 8.0, mutant enzyme W207L
2.06
-
Gln-Gln
-
pH 8.8, wild-type enzyme
4.67
-
Gln-Gln
-
pH 8.0, mutant enzyme F325A
12.62
-
Gln-Gln
-
pH 8.0, mutant enzyme E201D
29.53
-
Gln-Gln
-
pH 8.0, mutant enzyme W329A
0.31
-
Gln-Gln-Gln
-
-
0.53
-
Gln-Gln-Gln
-
-
0.148
-
Gln-Gln-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.041
-
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
0.045
-
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
0.094
-
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
0.106
-
Gln-Glu
-
pH 8.0, 30C
0.17
-
Gln-Glu
-
-
0.359
-
Gln-Glu
-
pH 8.0, 30C
0.47
-
Gln-Glu
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.607
-
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
0.61
-
Gln-Glu
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.077
-
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
0.102
-
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
0.039
-
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.081
-
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.055
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
0.061
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.094
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.107
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
0.069
-
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
0.113
-
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
0.079
-
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
0.103
-
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
0.047
-
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.1
-
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.16
-
Gln-Gly
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.247
-
Gln-Gly
-
pH 8.0, 30C
0.36
-
Gln-Gly
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.641
-
Gln-Gly
-
pH 8.0, 30C
1.1
-
Gln-Gly
-
-
1.88
-
Gln-Gly
-
-
0.102
-
Gln-Gly-Pro
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.13
-
Gln-Gly-Pro
-
pH 8.0, 30C
0.23
-
Gln-Gly-Pro
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.333
-
Gln-Gly-Pro
-
pH 8.0, 30C
0.09
-
Gln-His-Pro-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.068
-
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
0.092
-
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
0.034
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.053
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.054
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.118
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.401
-
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.409
-
Gln-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.409
-
Gln-NH2
-
pH 8.0, 30C
0.433
-
Gln-NH2
-
pH 8.0, 30C
0.446
-
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
1.1
-
Gln-NH2
-
wild-type enzyme
1.3
-
Gln-NH2
-
-
1.4
-
Gln-NH2
-
-
4.3
-
Gln-NH2
-
mutant enzyme H307Q
4.9
-
Gln-NH2
-
mutant enzyme H319Q
7.15
-
Gln-NH2
-
-
0.06
-
Gln-Phe-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.1
-
Gln-Phe-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.069
-
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.111
-
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.149
-
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
18.89
-
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
0.055
-
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
0.135
-
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
0.223
-
Gln-tert-butyl ester
-
pH 8.0, 30C
1.235
-
Gln-tert-butyl ester
-
pH 8.0, 30C
4.1
-
Gln-tert-butyl ester
-
-
6.7
-
Gln-tert-butyl ester
-
-
0.132
-
Gln-Thr-Ala
-
-
0.05
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
0.056
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.072
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.078
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
0.211
-
Gln-Tyr
-
pH 8.0, 30C
0.058
-
Gln-Tyr-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.063
-
Gln-Tyr-Ala
-
pH 8.0, 30C
0.08
-
Gln-Tyr-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.101
-
Gln-Tyr-Ala
-
pH 8.0, 30C
0.101
-
Gln-Tyr-Ala-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.153
-
Gln-Tyr-Ala-OH
-
37C, recombinant enzyme, expressed in Pichia pastoris
0.12
-
Gln-Val
-
-
0.196
-
Gln-Val
-
pH 8.0, 30C
0.019
-
glucagon(3-29)
-
pH 6.0, 30C, 1% DMSO
-
0.32
-
glutamine tert-butyl ester
-
-
0.063
-
gonadotropin releasing hormone
-
-
-
0.017
-
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.08
-
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
0.105
-
H-Gln-7-amino-4-methylcoumarin
Q84WV9, -
pH 8.0, 30C
0.294
-
H-Gln-Ala-OH
-
pH 8.0, 30C
1.838
-
H-Gln-Ala-OH
Q84WV9, -
pH 8.0, 30C
2.8
-
H-Gln-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
5
-
H-Gln-Ala-OH
-, Q9VRQ9
30C
0.487
-
H-Gln-Asp-Glu-Leu-NH2
-
pH 8.0, 30C
1.748
-
H-Gln-Asp-Glu-Leu-NH2
Q84WV9, -
pH 8.0, 30C
2.3
-
H-Gln-Asp-Glu-Leu-NH2
-, Q9VRQ9
30C
0.028
-
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
0.036
-
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
0.056
-
H-Gln-beta-naphthylamide
Q84WV9, -
pH 8.0, 30C
0.15
-
H-Gln-beta-naphthylamide
-, Q9VRQ9
30C, isoDromeQC
0.17
-
H-Gln-beta-naphthylamide
-, Q9VRQ9
30C
0.053
-
H-Gln-Gln-OH
-
pH 8.0, 30C
0.115
-
H-Gln-Gln-OH
-
pH 8.0, 30C
0.487
-
H-Gln-Gln-OH
Q84WV9, -
pH 8.0, 30C
0.94
-
H-Gln-Gln-OH
-, Q9VRQ9
30C, isoDromeQC
1.5
-
H-Gln-Gln-OH
-, Q9VRQ9
30C
0.08
-
H-Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
0.42
-
H-Gln-Glu-Ala-Phe-NH2
-, Q9VRQ9
30C
0.68
-
H-Gln-Glu-Ala-Phe-NH2
-, Q9VRQ9
30C, isoDromeQC
0.742
-
H-Gln-Glu-Ala-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.265
-
H-Gln-Glu-OH
-
pH 8.0, 30C
0.705
-
H-Gln-Glu-OH
-
pH 8.0, 30C
2.516
-
H-Gln-Glu-OH
Q84WV9, -
pH 8.0, 30C
29.5
-
H-Gln-Glu-OH
-, Q9VRQ9
30C
0.33
-
H-Gln-Glu-Tyr-Ala-NH2
Q84WV9, -
pH 8.0, 30C
0.065
-
H-Gln-Glu-Tyr-Phe-NH2
-, Q9VRQ9
30C
0.086
-
H-Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.41
-
H-Gln-Glu-Tyr-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.424
-
H-Gln-Gly-OH
-
pH 8.0, 30C
1.1
-
H-Gln-Gly-OH
-
pH 8.0, 30C
2.4
-
H-Gln-Gly-OH
-, Q9VRQ9
30C, isoDromeQC
2.64
-
H-Gln-Gly-OH
Q84WV9, -
pH 8.0, 30C
7.1
-
H-Gln-Gly-OH
-, Q9VRQ9
30C
0.214
-
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
0.59
1
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
1.17
-
H-Gln-Gly-Pro-OH
-, Q9VRQ9
30C, isoDromeQC
1.791
-
H-Gln-Gly-Pro-OH
Q84WV9, -
pH 8.0, 30C
4.1
-
H-Gln-Gly-Pro-OH
-, Q9VRQ9
30C
0.06
-
H-Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
0.08
-
H-Gln-Lys-Arg-Leu-NH2
-, Q9VRQ9
30C
0.448
-
H-Gln-Lys-Arg-Leu-NH2
Q84WV9, -
pH 8.0, 30C
0.48
-
H-Gln-NH2
-
pH 8.0, 30C
2.048
-
H-Gln-NH2
Q84WV9, -
pH 8.0, 30C
0.106
-
H-Gln-Phe-Ala-NH2
-
pH 8.0, 30C
0.44
-
H-Gln-Phe-Ala-NH2
Q84WV9, -
pH 8.0, 30C
0.128
-
H-Gln-Phe-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
0.35
-
H-Gln-Phe-Ala-OH
-, Q9VRQ9
30C
0.247
-
H-Gln-Ser-Tyr-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.72
-
H-Gln-Tyr-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
0.014
-
H-Gln-Val-Ala-OH
-
pH 8.0, 30C
0.1293
-
H-Gln-Val-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
0.14
-
H-Gln-Val-Ala-OH
-, Q9VRQ9
30C
0.282
-
H-Gln-Val-Ala-OH
Q84WV9, -
pH 8.0, 30C
0.028
-
L-Gln-2-naphthylamide
-
pH 8.0, 30C
0.039
-
L-Gln-2-naphthylamide
Q8P8M4, -
mutant enzyme E45Q, at pH 7.0 at 25C
0.04
-
L-Gln-2-naphthylamide
-, Q9CYK2
pH 8.0, 30C
0.056
-
L-Gln-2-naphthylamide
Q84WV9, -
pH 8.0, 30C
0.121
-
L-Gln-2-naphthylamide
Q8P8M4, -
wild type enzyme, at pH 7.0 at 25C
0.4
-
L-Gln-2-naphthylamide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.5
-
L-Gln-2-naphthylamide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0.048
-
L-Gln-7-amido-4-methylcoumarin
-, Q9CYK2
pH 8.0, 30C
0.05
-
L-Gln-7-amido-4-methylcoumarin
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0.08
-
L-Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
0.105
-
L-Gln-7-amido-4-methylcoumarin
Q84WV9, -
pH 8.0, 30C
0.3
-
L-Gln-7-amido-4-methylcoumarin
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.205
-
L-Gln-7-amino-4-methylcoumarin
-, Q9VRQ9
30C, isoDromeQC
0.31
-
L-Gln-7-amino-4-methylcoumarin
-, Q9VRQ9
30C
2.7
-
L-Gln-amide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
2.9
-
L-Gln-amide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.41
-
L-Gln-Gly
-, Q9CYK2
pH 8.0, 30C
1.1
-
L-Gln-Gly
-
pH 8.0, 30C
2.64
-
L-Gln-Gly
Q84WV9, -
pH 8.0, 30C
3.8
-
L-Gln-Gly
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.17
-
L-Gln-Gly-L-Pro
-, Q9CYK2
pH 8.0, 30C
0.59
1
L-Gln-Gly-L-Pro
-
pH 8.0, 30C
1.791
-
L-Gln-Gly-L-Pro
Q84WV9, -
pH 8.0, 30C
0.294
-
L-Gln-L-Ala
-
pH 8.0, 30C
0.4
-
L-Gln-L-Ala
-, Q9CYK2
pH 8.0, 30C
1.838
-
L-Gln-L-Ala
Q84WV9, -
pH 8.0, 30C
0.32
-
L-Gln-L-Arg-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
0.36
-
L-Gln-L-Asn-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
0.487
-
L-Gln-L-Asp-L-Glu-L-Leu-NH2
-
pH 8.0, 30C
1.74
-
L-Gln-L-Asp-L-Glu-L-Leu-NH2
Q84WV9, -
pH 8.0, 30C
0.053
-
L-Gln-L-Gln
-
pH 8.0, 30C
0.15
-
L-Gln-L-Gln
-, Q9CYK2
pH 8.0, 30C
0.487
-
L-Gln-L-Gln
Q84WV9, -
pH 8.0, 30C
6.5
-
L-Gln-L-Gln
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
20.4
-
L-Gln-L-Gln
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.265
-
L-Gln-L-Glu
-
pH 8.0, 30C
0.8
-
L-Gln-L-Glu
-, Q9CYK2
pH 8.0, 30C
2.4
-
L-Gln-L-Glu
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
2.516
-
L-Gln-L-Glu
Q84WV9, -
pH 8.0, 30C
4.2
-
L-Gln-L-Glu
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.08
-
L-Gln-L-Glu-L-Ala-L-Phe-NH2
-
pH 8.0, 30C
0.742
-
L-Gln-L-Glu-L-Ala-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.33
-
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
0.086
-
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
-
pH 8.0, 30C
0.41
-
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.06
-
L-Gln-L-Lys-L-Arg-L-Leu-NH2
-
pH 8.0, 30C
0.448
-
L-Gln-L-Lys-L-Arg-L-Leu-NH2
Q84WV9, -
pH 8.0, 30C
0.106
-
L-Gln-L-Phe-L-Ala-NH2
-
pH 8.0, 30C
0.44
-
L-Gln-L-Phe-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
0.247
-
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
0.16
-
L-Gln-L-Thr-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
0.072
-
L-Gln-L-Trp-L-Ala-NH2
-, Q9CYK2
pH 8.0, 30C
0.014
-
L-Gln-L-Val-L-Ala-NH2
-
pH 8.0, 30C
0.272
-
L-Gln-L-Val-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
0.48
-
L-Gln-NH2
-
pH 8.0, 30C
2.048
-
L-Gln-NH2
Q84WV9, -
pH 8.0, 30C
0.151
-
L-Gln-tert-butyl ester
-
pH 8.0, 30C
0.294
-
L-Gln-tert-butyl ester
Q84WV9, -
pH 8.0, 30C
1
-
L-Gln-tert-butyl ester
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
8.2
-
L-Gln-tert-butyl ester
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0.379
-
L-glutamine tert-butyl ester
-
pH 6.5
0.4
-
L-glutamine tert-butyl ester
-
pH 6.75
0.43
-
L-glutamine tert-butyl ester
-
pH 7.45
0.44
-
L-glutamine tert-butyl ester
-
pH 8.05
0.46
-
L-glutamine tert-butyl ester
-
pH 7.0 or pH 8.6
0.55
-
L-glutamine tert-butyl ester
-
pH 6.2
0.6
-
L-glutamine tert-butyl ester
-
pH 9.0
0.62
-
L-glutamine tert-butyl ester
-
pH 6.3
0.9
-
L-glutamine tert-butyl ester
-
pH 9.55
1.3
-
L-glutamine tert-butyl ester
-
pH 9.95
0.06
-
L-glutaminyl-2-naphthylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.051
-
L-glutaminyl-4-methylcoumarinylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
0.03
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.03
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.032
-
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.032
-
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
0.063
-
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
1.1
-
QAAE
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
1.6
-
QAAE
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.1
-
QAAF
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.8
-
QAAF
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.4
-
QAAR
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.2
-
QAEA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.7
-
QAEA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
2.1
-
QAFA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
2.5
-
QAFA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.1
-
QARA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
24.4
-
QARA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
1
-
QEAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
1.1
-
QEAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.16
-
QEDL
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.16
-
QEDL
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.029
-
QEYF
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.04
-
QEYF
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
1.9
-
QFAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
3.5
-
QFAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.1
-
QGGG
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
5.6
-
QGGG
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.9
-
QRAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
2.9
-
QRAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
0.088
-
[Gln1,Gly4]-thyrotropin releasing hormone
-
-
-
0.087
-
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
0.151
-
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
0.031
-
[Gln1]-gastrin
-
pH 8.0, 30C
-
0.033
-
[Gln1]-gastrin
-
pH 8.0, 30C
-
0.053
-
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
0.054
-
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
0.023
-
[Gln1]-neurotensin
-
pH 8.0, 30C
-
0.037
-
[Gln1]-neurotensin
-
pH 8.0, 30C
-
0.09
-
[Gln1]-thyrotropin releasing-hormone
-
pH 8.0, 30C
-
0.035
-
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00022
-
EFRH-NH2
-
at pH 6.5, 150000fold lower compared to Gln substrate
0.00042
-
EFRHHDSGYE-NH2
-
-
41.4
-
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, 1% DMSO
-
55
-
Gln(3)-amyloid-beta peptide 3-11 amide
-
pH 6.0, 30C, in absence of DMSO
-
62
-
Gln(3)-amyloid-beta peptide 3-21 amide
-
pH 6.0, 30C, 1% DMSO
-
40
-
Gln(3)-amyloid-beta peptide 3-40 amide
-
pH 6.0, 30C, 1% DMSO
-
0.0019
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305E
0.0042
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248Q
0.03
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305N
0.068
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D305A
0.085
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme D248A
0.84
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160G
1
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme E201D; pH 8.0, 25C, mutant enzyme H319L
5.3
-
Gln-2-naphthylamide
-
pH 8.0, 25C, mutant enzyme S160A
5.4
-
Gln-2-naphthylamide
-
pH 8.0, 25C, wild-type enzyme
5.3
-
Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
39.4
-
Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
1.27
-
Gln-Ala
-
-
47.6
-
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
57.2
-
Gln-Ala
-
pH 8.0, 30C
68.1
-
Gln-Ala
-
pH 8.0, 30C, 0.5 M KCl
69.8
-
Gln-Ala
-
pH 8.0, 30C
60.5
-
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
74.6
-
Gln-Ala-Ala-Ala-Ala-NH2
-
pH 8.0, 30C
76.3
-
Gln-Ala-Ala-NH2
-
pH 8.0, 30C
83.2
-
Gln-Ala-Ala-NH2
-
pH 8.0, 30C
55.3
-
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
78.5
-
Gln-Ala-Ala-Ser-Ala-Ala-NH2
-
pH 8.0, 30C
29.8
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
33.5
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
48.4
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C, 0.5 M KCl
49.2
-
Gln-Arg-Gly-Ile-NH2
-
pH 8.0, 30C
29.6
-
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
48.9
-
Gln-Arg-Tyr-Phe-NH2
-
pH 8.0, 30C
51.4
-
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
56.6
-
Gln-Asn-Gly-Ile-NH2
-
pH 8.0, 30C
20.6
-
Gln-beta-naphthylamide
-
pH 8.0, 30C
51.4
-
Gln-beta-naphthylamine
-
pH 8.0, 30C
0.43
-
Gln-Gln
-
pH 8.0, mutant enzyme W207L
0.848
-
Gln-Gln
-
-
0.87
-
Gln-Gln
-
pH 8.0, mutant enzyme E201D
1.35
-
Gln-Gln
-
pH 8.0, mutant enzyme W329A
2.32
-
Gln-Gln
-
pH 8.0, mutant enzyme W207F
2.7
-
Gln-Gln
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
7.3
-
Gln-Gln
-
pH 7.0, wild-type enzyme
7.35
-
Gln-Gln
-
pH 8.0, mutant enzyme R54W
7.91
-
Gln-Gln
-
pH 8.0, mutant enzyme F146A
8.56
-
Gln-Gln
-
pH 8.8, wild-type enzyme
8.6
-
Gln-Gln
-
-
8.63
-
Gln-Gln
-
pH 8.0, wild-type enzyme
8.66
-
Gln-Gln
-
protein expressed in Pichia pastoris, pH 8.0, 30C
9.39
-
Gln-Gln
-
pH 8.0, mutant enzyme Q304L
9.76
-
Gln-Gln
-
pH 7.5, wild-type enzyme
9.93
-
Gln-Gln
-
pH 8.5, wild-type enzyme
10
-
Gln-Gln
-
-
11.67
-
Gln-Gln
-
pH 8.0, mutant enzyme K144A
12.91
-
Gln-Gln
-
pH 8.0, mutant enzyme F325A
20.7
-
Gln-Gln
-
pH 8.0, 30C
43.2
-
Gln-Gln
-
pH 8.0, 30C
0.755
-
Gln-Gln-Gln
-
-
12.8
-
Gln-Gln-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
41.4
-
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
52.9
-
Gln-Gln-Tyr-Phe-NH2
-
pH 8.0, 30C
2.6
-
Gln-Glu
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
7.79
-
Gln-Glu
-
protein expressed in Pichia pastoris, pH 8.0, 30C
18.9
-
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
24.7
-
Gln-Glu
-
pH 8.0, 30C
44.4
-
Gln-Glu
-
pH 8.0, 30C, 0.5 M KCl
50.3
-
Gln-Glu
-
pH 8.0, 30C
46
-
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
53.7
-
Gln-Glu-Ala-Ala-NH2
-
pH 8.0, 30C
39
-
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
48.5
-
Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
45.6
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
45.8
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
53.6
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
58.5
-
Gln-Glu-Asp-Leu-NH2
-
pH 8.0, 30C
42.1
-
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
44.7
-
Gln-Glu-Tyr-Ala-NH2
-
pH 8.0, 30C
45.1
-
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
53.6
-
Gln-Glu-Tyr-NH2
-
pH 8.0, 30C
46
-
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
54.6
-
Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
0.368
-
Gln-Gly
-
-
1.65
-
Gln-Gly
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
4.57
-
Gln-Gly
-
protein expressed in Pichia pastoris, pH 8.0, 30C
13.2
-
Gln-Gly
-
pH 8.0, 30C
45.8
-
Gln-Gly
-
pH 8.0, 30C
4
-
Gln-Gly-Pro
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
11.4
-
Gln-Gly-Pro
-
protein expressed in Pichia pastoris, pH 8.0, 30C
25.3
-
Gln-Gly-Pro
-
pH 8.0, 30C
41.7
-
Gln-Gly-Pro
-
pH 8.0, 30C
83
-
Gln-His-Pro-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
55.4
-
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
75.9
-
Gln-His-Tyr-Phe-NH2
-
pH 8.0, 30C
31.6
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
33.4
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
48.2
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
58.1
-
Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C, 0.5 M KCl
0.36
-
Gln-NH2
-
mutant enzyme H319Q
0.388
-
Gln-NH2
-
mutant enzyme H307Q
0.435
-
Gln-NH2
-
unmutated enzyme
0.55
-
Gln-NH2
-
-
12.2
-
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
12.8
-
Gln-NH2
-
pH 8.0, 30C
13
-
Gln-NH2
-
-
20.7
-
Gln-NH2
-
30C, recombinant enzyme, expressed in Pichia pastoris
44.8
-
Gln-NH2
-
pH 8.0, 30C
45.2
-
Gln-NH2
-
pH 8.0, 30C, 0.5 M KCl
7.5
-
Gln-Phe-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
24.1
-
Gln-Phe-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
109
-
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
132
-
Gln-Phe-Ala-NH2
-
pH 8.0, 30C
18.8
-
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
31.7
-
Gln-Pro-Tyr-Phe-NH2
-
pH 8.0, 30C
52.8
-
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
64.9
-
Gln-Ser-Tyr-Phe-NH2
-
pH 8.0, 30C
6.7
-
Gln-tert-butyl ester
-
pH 8.0, 30C
16
-
Gln-tert-butyl ester
-
-
20.9
-
Gln-tert-butyl ester
-
-
49.4
-
Gln-tert-butyl ester
-
pH 8.0, 30C
47
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
50
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
133
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C, 0.5 M KCl
151.8
-
Gln-Trp-Ala-NH2
-
pH 8.0, 30C
94
-
Gln-Tyr
-
pH 8.0, 30C
7.7
-
Gln-Tyr-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
22.9
-
Gln-Tyr-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
104
-
Gln-Tyr-Ala
-
pH 8.0, 30C
125
-
Gln-Tyr-Ala
-
pH 8.0, 30C
125
-
Gln-Tyr-Ala-OH
-
30C, recombinant enzyme, expressed in Pichia pastoris
220
-
Gln-Tyr-Ala-OH
-
37C, recombinant enzyme, expressed in Pichia pastoris
17.2
-
Gln-Val
-
pH 8.0, 30C
10
-
glucagon(3-29)
-
pH 6.0, 30C, 1% DMSO
-
1.07
-
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
19
-
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
25
-
H-Gln-7-amino-4-methylcoumarin
Q84WV9, -
pH 8.0, 30C
16
-
H-Gln-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
17
-
H-Gln-Ala-OH
-, Q9VRQ9
30C
30
-
H-Gln-Ala-OH
-
pH 8.0, 30C
37.7
-
H-Gln-Ala-OH
Q84WV9, -
pH 8.0, 30C
13.1
-
H-Gln-Asp-Glu-Leu-NH2
-
pH 8.0, 30C
14
-
H-Gln-Asp-Glu-Leu-NH2
-, Q9VRQ9
30C
40
-
H-Gln-Asp-Glu-Leu-NH2
Q84WV9, -
pH 8.0, 30C
1.1
-
H-Gln-beta-naphthylamide
-, Q9VRQ9
30C, isoDromeQC
1.3
-
H-Gln-beta-naphthylamide
-, Q9VRQ9
30C
3.4
-
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
8
-
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
45
-
H-Gln-beta-naphthylamide
Q84WV9, -
pH 8.0, 30C
2.72
-
H-Gln-Gln-OH
-
pH 8.0, 30C
7
-
H-Gln-Gln-OH
-, Q9VRQ9
30C
7.4
-
H-Gln-Gln-OH
-, Q9VRQ9
30C, isoDromeQC
17
-
H-Gln-Gln-OH
-
pH 8.0, 30C
24
-
H-Gln-Gln-OH
Q84WV9, -
pH 8.0, 30C
11
-
H-Gln-Glu-Ala-Phe-NH2
-, Q9VRQ9
30C, isoDromeQC
12.7
-
H-Gln-Glu-Ala-Phe-NH2
-, Q9VRQ9
30C
14.9
-
H-Gln-Glu-Ala-Phe-NH2
-
pH 8.0, 30C
64
-
H-Gln-Glu-Ala-Phe-NH2
Q84WV9, -
pH 8.0, 30C
2.65
-
H-Gln-Glu-OH
-
pH 8.0, 30C
16.4
-
H-Gln-Glu-OH
-
pH 8.0, 30C
30
-
H-Gln-Glu-OH
Q84WV9, -
pH 8.0, 30C
38
-
H-Gln-Glu-OH
-, Q9VRQ9
30C
49
-
H-Gln-Glu-Tyr-Ala-NH2
Q84WV9, -
pH 8.0, 30C
6.9
-
H-Gln-Glu-Tyr-Phe-NH2
-, Q9VRQ9
30C
9.2
-
H-Gln-Glu-Tyr-Phe-NH2
-
pH 8.0, 30C
50
-
H-Gln-Glu-Tyr-Phe-NH2
Q84WV9, -
pH 8.0, 30C
1.66
-
H-Gln-Gly-OH
-
pH 8.0, 30C
2
8
H-Gln-Gly-OH
Q84WV9, -
pH 8.0, 30C
4.9
-
H-Gln-Gly-OH
-, Q9VRQ9
30C, isoDromeQC
9
-
H-Gln-Gly-OH
-, Q9VRQ9
30C
22
-
H-Gln-Gly-OH
-
pH 8.0, 30C
4.02
-
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
14
-
H-Gln-Gly-Pro-OH
-, Q9VRQ9
30C, isoDromeQC
15.7
-
H-Gln-Gly-Pro-OH
-
pH 8.0, 30C
16
-
H-Gln-Gly-Pro-OH
-, Q9VRQ9
30C
32
-
H-Gln-Gly-Pro-OH
Q84WV9, -
pH 8.0, 30C
7
-
H-Gln-Lys-Arg-Leu-NH2
-, Q9VRQ9
30C
20.6
-
H-Gln-Lys-Arg-Leu-NH2
-
pH 8.0, 30C
62
-
H-Gln-Lys-Arg-Leu-NH2
Q84WV9, -
pH 8.0, 30C
25
-
H-Gln-NH2
-
pH 8.0, 30C
37
-
H-Gln-NH2
Q84WV9, -
pH 8.0, 30C
55
-
H-Gln-Phe-Ala-NH2
-
pH 8.0, 30C
57
-
H-Gln-Phe-Ala-NH2
Q84WV9, -
pH 8.0, 30C
16
-
H-Gln-Phe-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
22
-
H-Gln-Phe-Ala-OH
-, Q9VRQ9
30C
44
-
H-Gln-Ser-Tyr-Phe-NH2
Q84WV9, -
pH 8.0, 30C
17.04
-
H-Gln-Tyr-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
3.02
-
H-Gln-Val-Ala-OH
-, Q9VRQ9
30C, isoDromeQC
5.3
-
H-Gln-Val-Ala-OH
-, Q9VRQ9
30C
10.9
-
H-Gln-Val-Ala-OH
-
pH 8.0, 30C
27
-
H-Gln-Val-Ala-OH
Q84WV9, -
pH 8.0, 30C
1.4
-
L-Gln-2-naphthylamide
Q8P8M4, -
wild type enzyme, at pH 7.0 at 25C
2.7
-
L-Gln-2-naphthylamide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
5.8
-
L-Gln-2-naphthylamide
Q8P8M4, -
mutant enzyme E45Q, at pH 7.0 at 25C
8
-
L-Gln-2-naphthylamide
-
pH 8.0, 30C
18.9
-
L-Gln-2-naphthylamide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
22
-
L-Gln-2-naphthylamide
-, Q9CYK2
pH 8.0, 30C
45
-
L-Gln-2-naphthylamide
Q84WV9, -
pH 8.0, 30C
0.45
-
L-Gln-7-amido-4-methylcoumarin
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
6
-
L-Gln-7-amido-4-methylcoumarin
-, Q9CYK2
pH 8.0, 30C
7.1
-
L-Gln-7-amido-4-methylcoumarin
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
19
-
L-Gln-7-amido-4-methylcoumarin
-
pH 8.0, 30C
25
-
L-Gln-7-amido-4-methylcoumarin
Q84WV9, -
pH 8.0, 30C
2.09
-
L-Gln-7-amino-4-methylcoumarin
-, Q9VRQ9
30C, isoDromeQC
7
-
L-Gln-7-amino-4-methylcoumarin
-, Q9VRQ9
30C
35.7
-
L-Gln-amide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
61.5
-
L-Gln-amide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
2
8
L-Gln-Gly
Q84WV9, -
pH 8.0, 30C
12.4
-
L-Gln-Gly
-, Q9CYK2
pH 8.0, 30C
22
-
L-Gln-Gly
-
pH 8.0, 30C
32
-
L-Gln-Gly
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
15.7
-
L-Gln-Gly-L-Pro
-
pH 8.0, 30C
21.6
-
L-Gln-Gly-L-Pro
-, Q9CYK2
pH 8.0, 30C
32
-
L-Gln-Gly-L-Pro
Q84WV9, -
pH 8.0, 30C
30
-
L-Gln-L-Ala
-
pH 8.0, 30C
37.7
-
L-Gln-L-Ala
Q84WV9, -
pH 8.0, 30C
42
-
L-Gln-L-Ala
-, Q9CYK2
pH 8.0, 30C
34
-
L-Gln-L-Arg-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
68
-
L-Gln-L-Asn-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
13.1
-
L-Gln-L-Asp-L-Glu-L-Leu-NH2
-
pH 8.0, 30C
40
-
L-Gln-L-Asp-L-Glu-L-Leu-NH2
Q84WV9, -
pH 8.0, 30C
0.27
-
L-Gln-L-Gln
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
17
-
L-Gln-L-Gln
-
pH 8.0, 30C
24
-
L-Gln-L-Gln
Q84WV9, -
pH 8.0, 30C
31.7
-
L-Gln-L-Gln
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
32
-
L-Gln-L-Gln
-, Q9CYK2
pH 8.0, 30C
0.021
-
L-Gln-L-Glu
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
12.8
-
L-Gln-L-Glu
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
16.4
-
L-Gln-L-Glu
-
pH 8.0, 30C
29
-
L-Gln-L-Glu
-, Q9CYK2
pH 8.0, 30C
30
-
L-Gln-L-Glu
Q84WV9, -
pH 8.0, 30C
14.9
-
L-Gln-L-Glu-L-Ala-L-Phe-NH2
-
pH 8.0, 30C
64
-
L-Gln-L-Glu-L-Ala-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
49
-
L-Gln-L-Glu-L-Tyr-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
9.2
-
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
-
pH 8.0, 30C
50
-
L-Gln-L-Glu-L-Tyr-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
20.6
-
L-Gln-L-Lys-L-Arg-L-Leu-NH2
-
pH 8.0, 30C
62
-
L-Gln-L-Lys-L-Arg-L-Leu-NH2
Q84WV9, -
pH 8.0, 30C
55
-
L-Gln-L-Phe-L-Ala-NH2
-
pH 8.0, 30C
57
-
L-Gln-L-Phe-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
44
-
L-Gln-L-Ser-L-Tyr-L-Phe-NH2
Q84WV9, -
pH 8.0, 30C
23
-
L-Gln-L-Thr-Gly-L-Ile-NH2
-, Q9CYK2
pH 8.0, 30C
41
-
L-Gln-L-Trp-L-Ala-NH2
-, Q9CYK2
pH 8.0, 30C
10.9
-
L-Gln-L-Val-L-Ala-NH2
-
pH 8.0, 30C
27
-
L-Gln-L-Val-L-Ala-NH2
Q84WV9, -
pH 8.0, 30C
25
-
L-Gln-NH2
-
pH 8.0, 30C
37
-
L-Gln-NH2
Q84WV9, -
pH 8.0, 30C
21
-
L-Gln-tert-butyl ester
-
pH 8.0, 30C
31.3
-
L-Gln-tert-butyl ester
Q84WV9, -
pH 8.0, 30C
64.9
-
L-Gln-tert-butyl ester
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
128
-
L-Gln-tert-butyl ester
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
33
-
L-glutamine tert-butyl ester
-
-
18.8
-
L-glutaminyl-2-naphthylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
5.4
-
L-glutaminyl-4-methylcoumarinylamide
-
30C, recombinant enzyme, expressed in Pichia pastoris
1.07
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
6.98
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein expressed in Pichia pastoris, pH 8.0, 30C
3.4
-
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
17.48
-
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
20
-
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
47.2
-
N1-naphthalen-2-yl-L-glutamamide
-
pH 8.0, 30C, 0.5 M KCl
0.054
-
QAAE
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
40
-
QAAE
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
8e-05
-
QAAF
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
183
-
QAAF
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
170
-
QAAR
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0042
-
QAEA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
40
-
QAEA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0064
-
QAFA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
102
-
QAFA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0032
-
QARA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
129
-
QARA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.007
-
QEAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
17.9
-
QEAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
6.4
-
QEDL
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
6.4
-
QEDL
-
protein expressed in Pichia pastoris, pH 8.0, 30C
3.3
-
QEYF
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
11.78
-
QEYF
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.084
-
QFAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
60
-
QFAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
30
-
QFRH-NH2
-
at pH 6.5
40
-
QFRH-NH2
-
at pH 6.5
42
-
QFRH-NH2
-
at pH 6.5
56
-
QFRH-NH2
-
at pH 6.5
0.012
-
QGGG
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
1.1
-
QGGG
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
0.0074
-
QRAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
-
66
-
QRAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
-
37.7
-
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
69.6
-
[Gln1]-fertilization promoting peptide
-
pH 8.0, 30C
-
31.6
-
[Gln1]-gastrin
-
pH 8.0, 30C
-
54.1
-
[Gln1]-gastrin
-
pH 8.0, 30C
-
69.2
-
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
72.4
-
[Gln1]-gonadotropin releasing-hormone
-
pH 8.0, 30C
-
37.7
-
[Gln1]-neurotensin
-
pH 8.0, 30C
-
48.8
-
[Gln1]-neurotensin
-
pH 8.0, 30C
-
82.8
-
[Gln1]-thyrotropin releasing-hormone
-
pH 8.0, 30C
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
15.9
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
20.2
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
20.9
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
21.1
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
21.9
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
27.6
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
45.3
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
49.9
-
39SrpL21 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
35
-
Acp33A protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
36
-
Acp33A protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
36.9
-
Acp33A protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
38
-
Acp33A protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
7.8
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
8.3
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
26.2
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
32
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
33.9
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
40.9
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
69
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
73.1
-
ALDH2 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
13.9
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
17.5
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
25.9
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
31.2
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0); isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
43.3
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
50.7
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
59.3
-
DIM-10 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
1.9e-05
-
EFRH-NH2
-
at pH 6.5
290363
3.7e-05
-
EFRH-NH2
-
at pH 6.5
290363
0.00017
-
EFRH-NH2
-
at pH 6.5
290363
0.00074
-
EFRH-NH2
-
at pH 6.5
290363
7.3e-05
-
EFRHHDSGYE-NH2
-
at low substrate concentrations
290364
9e-05
-
EFRHHDSGYE-NH2
-
-
290364
54
-
Gln-Gln
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
139559
95
-
Gln-Gln
-
protein expressed in Pichia pastoris, pH 8.0, 30C
139559
9
-
Gln-Glu
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
139562
16
-
Gln-Glu
-
protein expressed in Pichia pastoris, pH 8.0, 30C
139562
9
-
Gln-Gly
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
139563
28
-
Gln-Gly
-
protein expressed in Pichia pastoris, pH 8.0, 30C
139563
38
-
Gln-Gly-Pro
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
139564
111
-
Gln-Gly-Pro
-
protein expressed in Pichia pastoris, pH 8.0, 30C
139564
117
-
Gln-Phe-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
293138
403
-
Gln-Phe-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
293138
207
-
Gln-Tyr-Ala
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
218166
394
-
Gln-Tyr-Ala
-
protein expressed in Pichia pastoris, pH 8.0, 30C
218166
6.64
-
L-Gln-2-naphthylamide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
242033
12.6
-
L-Gln-2-naphthylamide
Q8P8M4, -
wild type enzyme, at pH 7.0 at 25C
242033
35
-
L-Gln-2-naphthylamide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
242033
166.1
-
L-Gln-2-naphthylamide
Q8P8M4, -
mutant enzyme E45Q, at pH 7.0 at 25C
242033
1.46
-
L-Gln-7-amido-4-methylcoumarin
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
242032
142
-
L-Gln-7-amido-4-methylcoumarin
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
242032
13
-
L-Gln-amide
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
21.2
-
L-Gln-amide
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
8.5
-
L-Gln-Gly
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
85854
0.013
-
L-Gln-L-Gln
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
242035
4.8
-
L-Gln-L-Gln
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
242035
0.0049
-
L-Gln-L-Glu
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
242036
5.4
-
L-Gln-L-Glu
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
242036
15.7
-
L-Gln-tert-butyl ester
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
242042
63.6
-
L-Gln-tert-butyl ester
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
242042
103
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
290411
311
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein expressed in Pichia pastoris, pH 8.0, 30C
290411
229
-
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
290410
554
-
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
290410
22
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
22.8
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
25
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
25.5
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
26.1
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
28.9
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
32.3
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
33.2
-
MCCB protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
4.2
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
4.6
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
6
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
7.4
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
20.1
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
20.9
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
21.5
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
23.8
-
NPLP4 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
0.034
-
QAAE
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
36
-
QAAE
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.0008
-
QAAF
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
219
-
QAAF
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
412
-
QAAR
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.02
-
QAEA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
55
-
QAEA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.0031
-
QAFA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
41
-
QAFA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.0013
-
QARA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
1215
-
QARA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.0067
-
QEAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
18
-
QEAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
55
-
QEDL
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
293140
104
-
QEDL
-
protein expressed in Pichia pastoris, pH 8.0, 30C
293140
123
-
QEYF
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
293139
413
-
QEYF
-
protein expressed in Pichia pastoris, pH 8.0, 30C
293139
0.24
-
QFAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
31
-
QFAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
98.9
-
QFRH-NH2
-
at pH 6.5
293386
100
-
QFRH-NH2
-
at pH 6.5
293386
135
-
QFRH-NH2
-
at pH 6.5
293386
145
-
QFRH-NH2
-
at pH 6.5
293386
0.0021
-
QGGG
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
9
-
QGGG
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
0.0026
-
QRAA
Q5NLA9
in 0.1 M MES, pH 6.0, temperature not specified in the publication
0
76
-
QRAA
Q1DDS6
in 0.1 M MES, pH 7.5, temperature not specified in the publication
0
26.2
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM Tris (pH 8.0)
0
30.2
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
0
40.9
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM Tris (pH 8.0)
0
43.4
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
0
50.2
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC mutant C136A/C158A, at 30C in 50 mM MOPS (pH 7.0)
0
53.3
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
55
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
0
88
-
rho-7 protein
-, Q86PD7, Q9VRQ9
isoform DromeQC mutant C113A/C136A, at 30C in 50 mM MOPS (pH 7.0)
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.253
-
(3,4-dichlorophenyl)-2-cyano-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
0.62
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
1.36
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(3,4-dimethoxyphenyl)guanidine
-
-
6.72
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-(trifluoromethyl)phenyl)guanidine
-
-
0.83
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-isopropylphenyl)guanidine
-
-
0.7
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(4-methoxyphenyl)guanidine
-
-
1.37
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-(cyclopropylmethyl)guanidine
-
-
1.53
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-methylguanidine
-
-
1.02
-
(3-(1H-imidazol-1-yl)propyl)-2-cyano-3-phenylguanidine
-
-
1.09
-
(3-(1H-imidazol-1-yl)propyl)-3-(4-bromophenyl)-2-cyanoguanidine
-
-
1.65
-
(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
0.13
-
(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-cyano-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)guanidine
-
-
0.295
-
1,4-bis-(imidazol-1-yl)methyl-2,5-dimethylbenzene
-
30C, pH 8.0
0.00112
-
1-(2,3-dihydro-1,4-benzodioxin-6-yl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00056
-
1-(2,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.184
-
1-(2-oxo-2-phenylethyl)-imidazole
-
30C, pH 8.0
0.00155
-
1-(3,4-dimethoxybenzyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00233
-
1-(3,4-dimethoxyphenyl)-3-(2-[1-(1H-imidazol-1-yl)cyclopropyl]ethyl)thiourea
-
pH 8.0, 30C
0.06
-
1-(3,4-dimethoxyphenyl)-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
0.0063
-
1-(3,4-dimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)thiourea
-
-
0.00034
-
1-(3,4-dimethoxyphenyl)-3-[(3R)-3-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
0.00076
-
1-(3,4-dimethoxyphenyl)-3-[(3S)-3-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
6e-05
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00049
-
1-(3,4-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]urea
-
pH 8.0, 30C
0.00055
-
1-(3,4-dimethoxyphenyl)-3-[4-(1H-imidazol-1-yl)butyl]thiourea
-
pH 8.0, 30C
0.041
-
1-(3,4-dimethoxyphenyl)-N-(3-(4-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
0.0026
-
1-(3,4-dimethoxyphenyl)-N-(3-(5-methyl-1H-imidazol-1-yl)propyl)cyclopropanecarbothioamide
-
-
0.00075
-
1-(3,5-dimethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.41
-
1-(3-aminopropyl)-imidazole
-
30C, pH 8.0
0.0018
-
1-(4-acetylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.00089
-
1-(4-ethoxyphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.0028
-
1-(4-ethylphenyl)-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.0062
-
1-(6-phenoxyhexyl)-1H-imidazole
-
-
0.000262
-
1-Benzylimidazole
Q9NXS2
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.000607
-
1-Benzylimidazole
Q9NXS2
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.0071
-
1-Benzylimidazole
-
30C, pH 8.0
0.013
-
1-Benzylimidazole
-, Q9VRQ9
pH 7
0.019
-
1-Benzylimidazole
-, Q9VRQ9
pH 8, isoDromeQC
0.048
-
1-Benzylimidazole
-, Q9VRQ9
pH 8
0.114
-
1-Benzylimidazole
-, Q9VRQ9
pH 7, isoDromeQC
0.12
-
1-methyl-5-(beta-aminoethyl)-imidazole
-
30C, pH 8.0
0.000101
-
1-Methylimidazole
-, Q9VRQ9
pH 7, isoDromeQC
0.03
-
1-Methylimidazole
-
30C, pH 8.0
0.036
-
1-Methylimidazole
-, Q9VRQ9
pH 7
0.103
-
1-Methylimidazole
-, Q9VRQ9
pH 8, isoDromeQC
0.109
-
1-Methylimidazole
-, Q9VRQ9
pH 8
0.049
-
1-vinylimidazole
-
30C, pH 8.0
0.0028
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(1-naphthyl)thiourea
-
pH 8.0, 30C
0.00034
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3,4,5-trimethoxyphenyl)thiourea
-
pH 8.0, 30C
0.00186
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(3-methoxyphenyl)thiourea
-
pH 8.0, 30C
0.0007
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methoxyphenyl)thiourea
-
pH 8.0, 30C
0.00214
-
1-[3-(1H-imidazol-1-yl)propyl]-3-(4-methylphenyl)thiourea
-
pH 8.0, 30C
0.00166
-
1-[3-(1H-imidazol-1-yl)propyl]-3-[4-(methylthio)phenyl]thiourea
-
pH 8.0, 30C
0.00097
-
1-[4-(benzyloxy)phenyl]-3-[3-(1H-imidazol-1-yl)propyl]thiourea
-
pH 8.0, 30C
0.23
-
2,3-dihydro-3-(3-(4-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
0.018
-
2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxoquinazolin-4(1H)-one
-
-
0.083
-
2,3-dihydro-6-methyl-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-5-phenyl-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
1.8
-
2-aminobenzimidazole
-
30C, pH 8.0
0.061
-
2-cyano(3,4,5-trimethoxyphenyl)-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)guanidine
-
-
0.082
-
2-cyano(3-(5-methyl-1H-imidazol-1-yl)propyl)-3-(3,4-dimethylphenyl)guanidine
-
-
0.065
-
2-cyano-1-[3-(5-methyl-1H-imidazol-1-yl)propyl]-4-phenylbenzene-1-guanidine
-
-
0.58
-
2-ethyl-4-methyl-imidazole
-
30C, pH 8.0
2.58
-
2-mercaptoethanol
-, Q9CYK2
-
0.165
-
2-methyl-benzylimidazole
-
30C, pH 8.0
0.3
-
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-2-thioxoquinazolin-4(1H)-one
-
-
0.36
-
3-(3-(1H-imidazol-1-yl)propyl)-2,3-dihydro-7-methyl-2-thioxothieno[3,2-d]pyrimidin-4(1H)-one
-
-
0.62
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-1,2,3,5,6,7-hexahydro-4H-cyclopenta[4,5]thieno[2,3-d]pyrimidin-4-one
-
-
0.9
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]thieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.34
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.818
-
3-[3-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
30C, pH 8.0
0.039
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8,9,10-octahydrocycloocta[4,5]-thieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.017
-
3-[3-(5-methyl-1H-imidazol-1-yl)propyl]-2-thioxo-2,3,5,6,7,8-hexahydro[1]benzothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.0023
-
4-(2-imidazol-1-yl-ethoxy)-benzoic acid
-
30C, pH 8.0
7.6
-
4-imidazole-carboxaldehyde
-
30C, pH 8.0
0.073
-
4-methylimidazole
-, Q9VRQ9
pH 8
15.5
-
5-amino-3H-imidazole-4-carboxylic acid amide
-
30C, pH 8.0
0.129
-
5-hydroxymethyl-4-methyl-imidazole
-
30C, pH 8.0
0.02
-
6-benzyl-2,3-dihydro-3-(3-(5-methyl-1H-imidazol-1-yl)propyl)-2-thioxothieno[2,3-d]pyrimidin-4(1H)-one
-
-
0.017
-
benzimidazole
-, Q9VRQ9
pH 7, isoDromeQC
0.124
-
benzimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.13
-
benzimidazole
-
pH 8.0, 30C
0.138
-
benzimidazole
-
30C, pH 8.0
0.192
-
benzimidazole
-, Q9CYK2
pH 8.0, 30C
0.199
-
benzimidazole
-
pH 8.0, 30C
0.25
-
benzimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.549
-
benzimidazole
-, Q9VRQ9
pH 7
1.145
-
benzimidazole
-, Q9VRQ9
pH 8, isoDromeQC
2.188
-
benzimidazole
-, Q9VRQ9
pH 8
0.0035
-
benzylimidazole
-
pH 8.0, 30C
0.0039
-
benzylimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.0062
-
benzylimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.0064
-
benzylimidazole
-, Q9CYK2
pH 8.0, 30C
0.0073
-
benzylimidazole
-
pH 8.0, 30C
1.824
-
Cacodylate
Q9NXS2
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
6.696
-
Cacodylate
Q9NXS2
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.042
-
cysteamine
-, Q9CYK2
pH 8.0, 30C
0.056
-
cysteamine
-, Q9VRQ9
pH 8
0.068
-
cysteamine
-
pH 8.0, 30C
0.069
-
cysteamine
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.154
-
cysteamine
-, Q9VRQ9
pH 7, isoDromeQC
0.205
-
cysteamine
-, Q9VRQ9
pH 8, isoDromeQC
0.301
-
cysteamine
-, Q9VRQ9
pH 7
3.25
-
EFRH-NH2
-
at pH 8.0
8.33
-
EFRH-NH2
-
at pH 8.0
24.64
-
EFRH-NH2
-
at pH 6.0
35.75
-
EFRH-NH2
-
at pH 6.0
4.69
-
EFRHHDSGYE-NH2
-
at pH 8.0
6.1
-
EFRHHDSGYE-NH2
-
at pH 8.0
8.32
-
EFRHHDSGYE-NH2
-
at pH 6.0
10.4
-
EFRHHDSGYE-NH2
-
at pH 6.0
19.3
-
ethanolamine
-, Q9CYK2
-
22
-
ethylenediamine
-, Q9CYK2
-
0.97
-
Gln-tert-butyl ester
-
pH 8.0, 30C, 0.5 M KCl
1.2
-
Gln-tert-butyl ester
-
pH 8.0, 30C
1.21
-
Gln-tert-butyl ester
-
pH 8.0, 30C
1.33
-
Gln-tert-butyl ester
-
pH 8.0, 30C, 0.5 M KCl
4.5
-
H-Gln-7-amino-4-methylcoumarin
-
pH 8.0, 30C
5.73
-
H-Gln-7-amino-4-methylcoumarin
-
-
1.3
-
H-Gln-beta-naphthylamide
-
pH 8.0, 30C
1.47
-
H-Gln-beta-naphthylamide
-
-
0.6
-
H-His-Trp-OH
-
30C, pH 8.0
14.5
-
imidazol-4-carbonic acid methylester
-
30C, pH 8.0
0.103
-
imidazole
-
30C, pH 8.0
0.103
-
imidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.116
-
imidazole
-
pH 8.0, 30C
0.16
-
imidazole
-, Q9CYK2
pH 8.0, 30C
0.219
-
imidazole
-
pH 8.0, 30C
0.235
-
imidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.304
-
imidazole
-, Q9VRQ9
pH 7
0.364
-
imidazole
-, Q9VRQ9
pH 8, isoDromeQC
0.671
-
imidazole
-, Q9VRQ9
pH 7, isoDromeQC
1.141
-
imidazole
-, Q9VRQ9
pH 8
1.54
-
L-Gln-2-naphthylamide
Q8P8M4, -
wild type enzyme, at pH 7.0 at 25C
5.06
-
L-Gln-2-naphthylamide
Q8P8M4, -
mutant enzyme E45Q, at pH 7.0 at 25C
4.47
-
L-glutaminyl-7-amido-4-methylcoumarin
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
1.57
-
L-glutaminyl-beta-naphthylamide
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
3.55
-
L-glutaminyl-beta-naphthylamide
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.85
-
L-histamine
-
30C, pH 8.0
0.56
-
L-histidinamide
-
30C, pH 8.0
4.4
-
L-histidine
-
30C, pH 8.0
1.53
-
L-Histidinol
-
30C, pH 8.0
0.023
-
methylimidazole
-, Q9CYK2
pH 8.0, 30C
0.052
-
methylimidazole
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.079
-
methylimidazole
-
pH 8.0, 30C
0.082
-
methylimidazole
-
protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30C, similar value with other protein versions
0.027
-
N,N-dimethylcysteamine
-
protein expressed in Pichia pastoris, pH 8.0, 30C
0.051
-
N-(-1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chloro-benzenamine
-
-
0.54
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.61
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-3,4-dimethoxy-benzenamine
-
-
0.54
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)-benzenamine
-
-
0.57
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-chlorobenzenamine
-
-
1.03
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-methoxybenzenamine
-
-
1.17
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)benzenamine
-
-
0.52
-
N-(1-(3-(1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
1.29
-
N-(1-(3-(4-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.067
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-2,3-dihydrobenzo[b][1,4]-dioxin-6-amine
-
-
0.034
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)-4-(trifluoromethyl)benzenamine
-
-
0.044
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)cyclohexanamine
-
-
0.042
-
N-(1-(3-(5-methyl-1H-imidazol-1-yl)propylamino)-2-nitrovinyl)naphthalen-1-amine
-
-
0.5
-
N-(3-(1H-imidazol-1-yl)propyl)-N-cyclohexyl-2-nitroethene-1,1-diamine
-
-
20.55
-
N-(3-(1H-imidazol-1-yl)propyl)-N-methyl-2-nitroethene-1,1-diamine
-
-
0.0012
-
N-(4-chlorophenyl)-N'-[2-(1H-imidazol-1-yl)propyl]-2-thioxoimidazolidin-4-one
-
30C, pH 8.0
0.167
-
N-(trimethylsilyl)-imidazole
-
30C, pH 8.0
0.107
-
N-Acetylimidazole
-
30C, pH 8.0
0.174
-
N-benzoylimidazole
-
30C, pH 8.0
0.0109
-
N-diethylcysteamine
-, Q9CYK2
-
0.093
-
N-diethylcysteamine
-, Q9VRQ9
pH 8
0.405
-
N-diethylcysteamine
-, Q9VRQ9
pH 8, isoDromeQC
0.022
-
N-dimethylcysteamine
-
pH 8.0, 30C
0.029
-
N-dimethylcysteamine
-, Q9CYK2
-
0.063
-
N-dimethylcysteamine
-, Q9VRQ9
pH 8, isoDromeQC
0.079
-
N-dimethylcysteamine
-, Q9VRQ9
pH 8
0.08
-
N-dimethylcysteamine
-, Q9VRQ9
pH 7
0.013
-
N-methylimidazole
-
pH 8.0, 30C
0.017
-
N-omega-acetylhistamine
-
30C, pH 8.0
0.0391
-
N-omega-acetylhistamine
-, Q9VRQ9
pH 7
0.084
-
N-omega-acetylhistamine
-, Q9VRQ9
pH 8
0.14
-
N-omega-acetylhistamine
-, Q9VRQ9
pH 8, isoDromeQC
0.827
-
N-omega-acetylhistamine
-, Q9VRQ9
pH 7, isoDromeQC
0.002
-
N-[3-(1H-imidazol-1-yl)propyl]-5-methoxy-1,3-benzothiazol-2-amine
-
pH 8.0, 30C
0.00157
-
N-[3-(1H-imidazol-1-yl)propyl]-6-methoxy-1,3-benzothiazol-2-amine
-
pH 8.0, 30C
0.001698
-
Nomega-acetylhistamine
Q9NXS2
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.005748
-
Nomega-acetylhistamine
Q9NXS2
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
0.078
-
oxalic acid diimidazolidide
-
30C, pH 8.0
9.7e-05
-
P150/03
-, Q9VRQ9
pH 8, isoDromeQC
-
0.00094
-
P150/03
-, Q9VRQ9
pH 7
-
0.00314
-
P150/03
-, Q9VRQ9
pH 8
-
0.145
-
P150/03
-, Q9VRQ9
pH 7, isoDromeQC
-
9.5e-05
-
PBD150
Q9NXS2
secretory glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
-
0.0001
-
PBD150
-
pH 8.0, 30C
-
0.0001
-
PBD150
-
wild type enzyme, in 50 mM Tris, pH 8.0, at 30C
-
0.0001013
-
PBD150
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MOPS (pH 7.0)
-
0.0001093
-
PBD150
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM Tris (pH 8.0)
-
0.000114
-
PBD150
-
wild type enzyme, in 50 mM Tris, pH 8.0, at 30C
-
0.0003706
-
PBD150
-, Q86PD7, Q9VRQ9
isoform isoDromeQC, at 30C in 50 mM MES (pH 6.0)
-
0.000944
-
PBD150
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MOPS (pH 7.0)
-
0.001817
-
PBD150
Q9NXS2
Golgi-resident glutaminyl cyclase, at 25C in 50 mM Tris-HCl, pH 8.0
-
0.00248
-
PBD150
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM MES (pH 6.0)
-
0.003139
-
PBD150
-, Q86PD7, Q9VRQ9
isoform DromeQC, at 30C in 50 mM Tris (pH 8.0)
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
11.2
-
-, Q9CYK2
pH 8.0, 30C, recombinant enzyme
18.7
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
cyclization of glutamic acid
6
-
Q5NLA9
-
6.8
-
-
EFRH-NH2 as substrate
7.2
7.5
-
-
7.3
-
-
pituitary enzyme
7.3
-
-, Q86PD7, Q9VRQ9
isoform DromeQC
7.5
7.8
-
Gln-peptide as substrate
7.5
8
-
for kcat/KM
7.5
8
-, Q86PD7, Q9VRQ9
isoform isoDromeQC
8
-
-
-
8
-
-
cyclization of glutamine
8
-
Q1DDS6
-
8.5
-
-
enzyme from spleen
8.5
-
-
enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/L NaCl
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8.3
-
pH 6.5: about 50% of maximal activity, pH 8.3: about 40% of maximal activity, pituitary enzyme
7.5
9.3
-
pH 7.5: about 55% of maximal activity, pH 9.3: about 45% of maximal activity, enzyme from spleen
additional information
-
-
activity changes very slightly in the pH range between 4.5 and 10
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
secretory and non-secretory cell line P3X63Ag8
Manually annotated by BRENDA team
-
high expression of QC in neurons of several cell layers of the cortex and hippocampus
Manually annotated by BRENDA team
-
slightly lower glutaminyl cyclase activity in cerebrospinal fluid of multiple sclerosis patients compared to controls. The presence of glutaminyl cyclase in cerebrospinal fluid can stabilize peptides from degradation by aminopeptidases
Manually annotated by BRENDA team
-
high expression of QC in neurons of several cell layers of the cortex and hippocampus
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
-
plasma cell leukemia cell line
Manually annotated by BRENDA team
-
; estimated as a fraction of papaya latex
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
additional information
-
ubiquitous expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-, Q86PD7, Q9VRQ9
isoform DromeQC
-
Manually annotated by BRENDA team
-
exclusively localized within the Golgi complex, obviously retained by the N-terminus. Retention in the Golgi complex suggests involvement in the protein maturation machinery
Manually annotated by BRENDA team
-, Q86PD7, Q9VRQ9
isoform isoDromeQC
Manually annotated by BRENDA team
-
a 40000 Da enzyme form is exclusively detected in soluble pituitary extract, a 32000 Da enzyme form is detected in soluble fraction of all tissues which show immunoreactivity. The difference in the two immunoreacive proteins lies in the proteolytic cleavage within a hydrophilic region approximately 80 amino acids from the carboxy-terminus
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1)
Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30000
-
-
fraction are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography is carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry
37520
-
-
mass spectrometry analysis
48500
-
-
gel filtration
55000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 32980, native, glycosylated enzyme, SDS-PAGE; x * 33000, native, glycosylated enzyme, SDS-PAGE
?
-
x * 40000, SDS-PAGE; x * 40876, calculation from nucleotide sequence
?
-
x * 38795, deglycosylated enzyme, MALDI-TOF mass analysis
?
-
x * 37000, SDS-PAGE
?
-
x *32000, or x * 34000, SDS-PAGE, different glycosylation forms of recombinant enzyme. x * 29000, SDS-PAGE of deglycosylated enzyme, x * 29381, calculated
?
Q84WV9, -
x *32000, or x * 34000, SDS-PAGE, different glycosylation forms of recombinant enzyme. x * 29000, SDS-PAGE of deglycosylated enzyme, x * 29945, calculated
?
Q1DDS6
x * 30000, SDS-PAGE; x * 31100, MALDI-TOF mass spectrometry
?
Q5NLA9
x * 29900, MALDI-TOF mass spectrometry; x * 30000, SDS-PAGE
additional information
-
enzyme contains extensive beta-sheet structure and is likely to have only short immobile loops connecting ist beta-strands
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
treatment of recombinant enzyme with endoglycosidase H results in decrease of mass by 2 kDa. Deglycosylated enzyme has similar kinetic parameters for conversion of Gln-Gln and Gln-7-amido-4-methylcoumarin as native enzyme
glycoprotein
A7ISW2, -
sequence contain potential N-glycosylation sites at Asn53, Asn292, and Asn352
proteolytic modification
A7ISW2, -
sequence contains an ambiguous signal peptide cleavage site leading to a mature enzyme of 345, 340 or 335 amino acids
glycoprotein
A7ISW1, -
sequence contain potential N-glycosylation sites at Asn53, Asn292, and Asn352
proteolytic modification
A7ISW1, -
sequence contains an ambiguous signal peptide cleavage site leading to a mature enzyme of 345, 340 or 335 amino acids
glycoprotein
-
-
glycoprotein
-
presence of terminal mannose residues, mannose and N-acetylglucosamine in a 3:1 molar ratio
glycoprotein
-
-
glycoprotein
-
deglycosylation has no effect on enzymatic activity
glycoprotein
-
N-glycan sugar moiety is bound to Asn49 in the recombinant enzyme. Presence or absence of glycan has no apparent effect on structure, stability, or enzymatic activity of the enzyme
glycoprotein
-
-
glycoprotein
-, Q9CYK2
sequence contains a potential glycosylation site at Asp50
glycoprotein
-
-
glycoprotein
-
-
glycoprotein
Q84WV9, -
treatment of recombinant enzyme with endoglycosidase H results in decrease of mass by 2 kDa. Deglycosylated enzyme has similar kinetic parameters for conversion of Gln-Gln and Gln-7-amido-4-methylcoumarin as native enzyme
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters A = 62.82 A, b = 81.23 A, c = 108.17 A and two molecules per asymmetric unit, resolution 1.7 A
-
hatbox-shaped molecule that forms an unusual five-fold beta-propeller traversed by a central channel
-
structure determinantion by single wavelength anomalous diffraction technique using sulfur and zinc as anomalous scatterers. The zinc ion present is bound via an octahedral coordination into an elongated cavity along the pseudo 5fold axis of the beta-propeller fold. The active site is located at the C-terminal entrance of the enzymes central tunnel with residues W83, W110, Q24, E69, N155, K225, F22, and F67 in the entrance
-
hanging drop vapor diffusion method, using 10% (w/v) PEG 35000 and 100 mM Tris (pH 8.5) for isoform DromeQC; hanging drop vapor diffusion method, using 200 mM MnCl2, 30% (w/v) PEG 4000, and 100 mM Tris (pH 8.5) for isoform isoDromeQC
-, Q86PD7, Q9VRQ9
hanging drop vapor diffusion method, using 100 mM imidazole (pH 8.0), 30% (v/v) 2-methyl-2,4-pentanediol, and 11% (w/v) 4000 PEG
-
hanging drop vapor-diffusion method. The space group of the crystal is R32, with typical unit cells of a = b = 119 A and c = 333A, in which an asymmetric unit comprises two human glutaminyl cyclase molecules. Crystal structures of the enzyme bound to inhibitors: 1-vinylimidazole, 1-benzylimidazole and N-omega-acetylhistamine
-
hanging-drop vapor diffusion
-
mutant enzyme W207F, hanging drop vapor diffusion method, using 1.7 M (NH4)2SO4, 4% (v/v) dioxane, and 100 mM MES, pH 6.5, at 25C; sitting drop vapor diffusion method, using either 30% (w/v) PEG 200, 5% (w/v) PEG 3000, and 0.1 M MES, pH 6.0, or 50% (w/v) PEG 200, 0.2 M MgCl2, and 0.1 M cacodylate, pH 6.5
Q9NXS2
hanging drop vapor diffusion method, using 100 mM sodium acetate (pH 5.3), 200 mM ammonium sulfate, and 12% (w/v) 2000 MME-PEG
-
hanging drop vapor diffusion method, using 0.1 M MES and 30% (w/v) PEG-4000
Q1DDS6
mutant enzyme E89A, hanging drop vapor diffusion method, using 50 mM imidazole and 0.8 M sodium citrate, pH 8.7, at 25C
Q8P8M4, -
hanging drop vapor diffusion method, using 0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate and 12% (w/v) PEG-4000
Q5NLA9
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
9
Q8P8M4, -
the enzyme possesses relatively high activities between pH 5.0 and 7.5, but exhibits significantly decreased activities above pH 8.0 and below pH 5.0
4
-
-
30C, 30 min, about 10% loss of activity
5
6
-
30C, 30 min, about 30% loss of activity
5.5
9
-, Q9CYK2
stable for at least 30 min at 30C, both native and recombinant enzyme. At pH values below or above, rapid inactivation. 0.02 mM ZnSO4 partially prevents denaturation in the acidic range
5.5
-
-
30C, 30 min, about 70% loss of activity
6
-
-
30C, 30 min, about 50% loss of activity
6.5
8
-
30C, 30 min, about 10% loss of activity
6.5
-
-
30C, 30 min, about 40% loss of activity
7
9
-
30C, 30 min, less than 20% loss of activity
8.5
-
-
30C, 30 min, about 20% loss of activity
9
10
-
30C, 30 min, about 15% loss of activity
9.5
-
-
30C, 30 min, about 50% loss of activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
75
Q8P8M4, -
the enzyme is only stable below 40C and exhibits remarkable instability above 50C
65
-
-
pH 8.8, several hours, no inactivation
85
-
-
heat stability of QCT up to 85C
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
complete unfolding of the enzyme requires a combination of an acidic medium and chemical denaturant such as urea or guanidine hydrochloride
-
resistance to denaturation induced by guanidine-HCl
-
treatment with trypsin and chymotrypsin at pH 8.2 and 8.0 respectively, for 16 h at 37C: enzyme is recovered in its native form
-
after a 24-h incubation in 1 M guanidine hydrochloride the recombinant enzyme becomes aggregated
Q8P8M4, -
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration
-
; Ni-NTA column chromatography and Sephacryl S-100 gel filtration
Q9NXS2
chelating Sepharose column chromatography and butyl Sepharose column chromatography
-
hanging-drop vapour-diffusion method, wild-type and mutant enzymes
-
His-tagged version
-
of 6His-tagged enzyme expressed in Pichia pastoris and enzyme expressed in Escherichia coli
-
hydrophobic interaction chromatography, ion exchange chromatography, gel filtration
-
recombinant enzyme
-, Q9CYK2
Q-Sepharose column chromatography
Q1DDS6
Ni-NTA column chromatography
Q8P8M4, -
Q-Sepharose column chromatography
Q5NLA9
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Pichia pastoris
-
expression in Escherichia coli as either His-tagged enzyme with three different signal peptides and in fusions with three different signal peptides and in fusion with thioredoxin, glutathione S-transferase, and (pre-)maltose-binding protein. In all cases, the expressed protein is either undetectable or insoluble. Expression in Pichia pastoris of the enzyme fused to the alpha-factor leader results in low levels of activity. Extracellular expression of the enzyme in the insect cell/baculovirus system is sucessfull. Enzyme N-terminally fused to a combined secretion signal/His-tag peptide is correctly processed by the host signal peptidase and the His-tag can subsequently be removed with dipeptidyl peptidase I
-
expressed in Escherichia coli M15 cells (isoform isoDromeQC); expressed in Pichia pstoris strain X33 (isoform DromeQC)
-, Q86PD7, Q9VRQ9
heterologously expressed in Pichia pastoris and Escherichia coli; heterologously expressed in Pichia pastoris and Escherichia coli
-, Q9VRQ9
; expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells
Q9NXS2
cloned into the Escherichia coli expression vectors pMALc2 and pET19b. Expression of this cDNA in either vector results in the production of a glutaminyl cyclase fusion protein which is enzymatically active and reacts with anti-bovine glutaminyl cyclase antisera
-
expressed in HEK-293 cell, expressed in Pichia pastoris
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain X33
-
expression in Drosophila S2 cells
-
expression in Escherichia coli
-
expression in Pichia pastoris. In Escherichia coli only 50% of the protein does not contain a disulfide bond that is present in the enzyme expressed in Pichia pastoris
-
h-isoQC, lacking the N-terminal signal anchor and the short cytosolic tail, is expressed as a fusion protein in Escherichia coli
-
modified N-terminal region, His-tagged version, expressed in Pichia pastoris
-
-
-, Q9CYK2
expressed in Pichia pastoris
-
modified N-terminal region, His-tagged version, expressed in Pichia pastoris
-
expressed in Escherichia coli BL21(DE3) cells
Q1DDS6
expressed in Pichia pastoris
-
expression in Pichia pastoris
Q84WV9, -
expressed in Escherichia coli BL21(DE3) cells
Q8P8M4, -
expressed in Escherichia coli BL21(DE3) cells
Q5NLA9
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C113A/C136A
-, Q86PD7, Q9VRQ9
mutant of isoform DromeQC lacking a disulfide bond, without influence on the stability of the enzyme
C136A/C158A
-, Q86PD7, Q9VRQ9
mutant of isoform isoDromeQC lacking a disulfide bond, without influence on the stability of the enzyme
C369A
-
improvement of enzyme secretion (expressed in Pichia pastoris)
D248A
-
no activity
D248A
-
kcat/KM for Gln-2-naphthylamide is 136fold lower than wild-type value
D248Q
-
kcat/KM for Gln-2-naphthylamide is 7123fold lower than wild-type value
D305A
-
kcat/KM for Gln-2-naphthylamide is 1493fold lower than wild-type value
D305E
-
kcat/KM for Gln-2-naphthylamide is 21000fold lower than wild-type value
D305L
-
no activity
D305N
-
kcat/KM for Gln-2-naphthylamide is 772fold lower than wild-type value
E201D
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 200fold lower than wild-type ratio
E201D
-
kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value
E201Q
-
almost no catalytic activity
F146A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.43fold lower than wild-type ratio
F325A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 4.9fold lower than wild-type ratio
F325A
-
the mutant shows a 190fold increase in Ki of PBD150 compared to the wild type enzyme
F325N
-
the mutant shows a 815fold increase in Ki of PBD150 compared to the wild type enzyme
F325Y
-
the mutant shows a 381fold increase in Ki of PBD150 compared to the wild type enzyme
H140Q
-
inactive enzyme
H307Q
-
mutant enzyme with increased KM-value
H319L
-
kcat/KM for Gln-2-naphthylamide is 15fold lower than wild-type value
H319Q
-
mutant enzyme with increased KM-value
H330Q
-
inactive enzyme
I303A
-
the mutation results in a 66.3fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303F
-
the mutation results in a 6.2fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303N
-
the mutation results in a 112fold increase in the Ki value of PBD150 compared to the wild type enzyme
I303V
-
the mutation results in a 1.9fold increase in the Ki value of PBD150 compared to the wild type enzyme
I73N
-
artificial glycosylation site
I73N/C369A
-
further improvement of enzyme secretion (expressed in Pichia pastoris)
K144A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.7fold lower than wild-type ratio
K144A
-
the mutant shows a 1.21fold increase in Ki of PBD150 compared to the wild type enzyme
K144M
-
the mutant shows a 335fold increase in Ki of PBD150 compared to the wild type enzyme
K144R
-
the mutant shows a 237fold increase in Ki of PBD150 compared to the wild type enzyme
N49A
-
mutant enzyme is not glycosylated, kinetic properties are indistinguishable from unmutated enzyme
Q304L
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.7fold lower than wild-type ratio
R54W
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 1.4fold lower than wild-type ratio
S160A
-
kcat/KM for Gln-2-naphthylamide is 3fold lower than wild-type value
S160G
-
kcat/KM for Gln-2-naphthylamide is 68fold lower than wild-type value
S323A
-
the mutation leads to a significant decrease (0.2fold) in the Ki value of PBD150 compared to the wild type enzyme
S323T
-
the mutation leads to a significant decrease (0.21fold) in the Ki value of PBD150 compared to the wild type enzyme
S323V
-
the mutation leads to a strong decrease (0.074fold) in the Ki value of PBD150 compared to the wild type enzyme
W207F
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 3.45fold lower than wild-type ratio
W207F
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W207F
Q9NXS2
the mutation reduces the turnover rate (kcat) of the enzyme by about 3.7fold compared to the wild type enzyme
W207L
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 56fold lower than wild-type ratio
W207L
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W207Q
-
the mutation alters substrate conversion significantly, while the binding constants of inhibitors such as the highly potent PBD150 are minimally affected
W329A
-
the ratio of turnover-number to KM-value for Gln-Gln at pH 8.0 is 297fold lower than wild-type ratio
W329F
-
the mutation leads to a significant decrease (0.56fold) in the Ki value of PBD150 compared to the wild type enzyme
W329Y
-
the mutation does not affect the Ki of PBD150 compared to the wild type enzyme
Y299A
-
the mutant shows a 122fold increase in Ki of PBD150 compared to the wild type enzyme
Y299F
-
the mutant shows a 220fold increase in Ki of PBD150 compared to the wild type enzyme
I74N
-
artificial glycosylation site
Q46E
Q1DDS6
the mutants pH optimum is shifted towards lower pH values, although the activity towards L-glutamine-2-naphtylamine is some 1000fold lower than the wild type
E45A
Q8P8M4, -
the mutation leads to a drop in the enzyme activity (1.12% activity compared to the wild type enzyme)
E45Q
Q8P8M4, -
the mutation increases the enzyme activity by an order of magnitude (1079.68% activity compared to the wild type enzyme)
E89A
Q8P8M4, -
the mutant exhibits 5.46% activity compared to the wild type enzyme
F43A
Q8P8M4, -
the mutant exhibits 5.46% activity compared to the wild type enzyme
F87A
Q8P8M4, -
the mutant exhibits 3.87% activity compared to the wild type enzyme
W103A
Q8P8M4, -
the mutant exhibits 3.05% activity compared to the wild type enzyme
E45A
Xanthomonas campestris ATCC33913
-
the mutation leads to a drop in the enzyme activity (1.12% activity compared to the wild type enzyme)
-
E45Q
Xanthomonas campestris ATCC33913
-
the mutation increases the enzyme activity by an order of magnitude (1079.68% activity compared to the wild type enzyme)
-
E89A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 5.46% activity compared to the wild type enzyme
-
F87A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 3.87% activity compared to the wild type enzyme
-
W103A
Xanthomonas campestris ATCC33913
-
the mutant exhibits 3.05% activity compared to the wild type enzyme
-
E48Q
Q5NLA9
the mutants pH optimum is shifted towards higher pH values
additional information
-
N53A and N123A: no expression of either of the mutants is detected
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
-
QCT would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease
medicine
-
inhibition of glutaminyl cyclase offers a new therapeutic option for the treatment of Alzheimers disease and provides implications for other amyloidoses, such as familial Danish dementia
medicine
-
the enzyme is a pharmacological target for Alzheimers disease therapy