Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
A197D | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
F200A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
F200S | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
F200Y | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
G198A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
G198Y | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
M126I | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
M137L | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
Q9A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
R122A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
R122K | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
T57V | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
V98Q | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
malonyl-CoA | pH 6.8, recombinant mutant M137L | Streptomyces coelicolor | |
0.019 | - |
malonyl-CoA | pH 6.8, recombinant wild-type enzyme | Streptomyces coelicolor | |
0.023 | - |
malonyl-CoA | pH 6.8, recombinant mutant M126I | Streptomyces coelicolor | |
0.032 | - |
malonyl-CoA | pH 6.8, recombinant mutant V98Q | Streptomyces coelicolor | |
0.034 | - |
malonyl-CoA | pH 6.8, recombinant mutant A197D | Streptomyces coelicolor | |
0.05 | - |
malonyl-CoA | pH 6.8, recombinant mutant F200S | Streptomyces coelicolor | |
0.06 | - |
malonyl-CoA | pH 6.8, recombinant mutants G198Y and G198A | Streptomyces coelicolor | |
0.075 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant F200Y | Streptomyces coelicolor | |
0.083 | - |
malonyl-CoA | pH 6.8, recombinant mutant F200A | Streptomyces coelicolor | |
0.095 | - |
malonyl-CoA | pH 6.8, recombinant mutant F200Y | Streptomyces coelicolor | |
0.1 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutants M137L and F200S | Streptomyces coelicolor | |
0.12 | - |
[acyl-carrier protein] | pH 6.8, recombinant wild-type enzyme and mutant V98Q | Streptomyces coelicolor | |
0.13 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutants A197D and G198Y | Streptomyces coelicolor | |
0.15 | - |
malonyl-CoA | pH 6.8, recombinant mutant R122A | Streptomyces coelicolor | |
0.15 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutants M126I and G198A | Streptomyces coelicolor | |
0.2 | - |
malonyl-CoA | pH 6.8, recombinant mutant Q9A | Streptomyces coelicolor | |
0.24 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant F200A | Streptomyces coelicolor | |
0.4 | - |
malonyl-CoA | pH 6.8, recombinant mutant T57V | Streptomyces coelicolor | |
0.57 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant T57V | Streptomyces coelicolor | |
0.7 | - |
malonyl-CoA | pH 6.8, recombinant mutant R122K | Streptomyces coelicolor | |
0.92 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant Q9A | Streptomyces coelicolor | |
1.1 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant R122A | Streptomyces coelicolor | |
3.5 | - |
[acyl-carrier protein] | pH 6.8, recombinant mutant R122K | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | Streptomyces coelicolor | enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase | CoA + malonyl-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Streptomyces coelicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | putative active site structure and involved catalytic residues | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase | Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + [acyl-carrier protein] | the active site is rigorously ordered around the acyl-thioester moiety of the acyl-CoA to facilitate rapid and efficient transacylation to an ACP | Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA:acyl carrier protein transacylase | - |
Streptomyces coelicolor |
MAT | - |
Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
malonyl-CoA | pH 6.8, recombinant mutant F200S | Streptomyces coelicolor | |
0.3 | - |
malonyl-CoA | pH 6.8, recombinant mutant M137L | Streptomyces coelicolor | |
0.35 | - |
malonyl-CoA | pH 6.8, recombinant mutant R122A | Streptomyces coelicolor | |
0.4 | - |
malonyl-CoA | pH 6.8, recombinant mutants G198Y | Streptomyces coelicolor | |
0.7 | - |
malonyl-CoA | pH 6.8, recombinant mutant Q9A | Streptomyces coelicolor | |
0.94 | - |
malonyl-CoA | pH 6.8, recombinant mutant F200A | Streptomyces coelicolor | |
3 | 6 | malonyl-CoA | pH 6.8, recombinant mutant F200Y | Streptomyces coelicolor | |
3 | - |
malonyl-CoA | pH 6.8, recombinant mutant M126I | Streptomyces coelicolor | |
11 | - |
malonyl-CoA | pH 6.8, recombinant mutant R122K | Streptomyces coelicolor | |
12 | - |
malonyl-CoA | pH 6.8, recombinant mutants G198A | Streptomyces coelicolor | |
25 | - |
malonyl-CoA | pH 6.8, recombinant mutant T57V | Streptomyces coelicolor | |
70 | - |
malonyl-CoA | pH 6.8, recombinant mutant A197D | Streptomyces coelicolor | |
95 | - |
malonyl-CoA | pH 6.8, recombinant mutant V98Q | Streptomyces coelicolor | |
100 | - |
malonyl-CoA | pH 6.8, recombinant wild-type enzyme | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Streptomyces coelicolor |