Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, enhances activity, relative activity: 143% | Helianthus annuus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM, 87% residual activity; slightly decreases activity, relative activity: 87.5% (2 mM), 37% (5 mM) | Helianthus annuus | |
Mn2+ | 2 mM, 82% residaul activity; slightly decreases activity, relative activity: 81.5% (2 mM) | Helianthus annuus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.354 | - |
glycerol 3-phosphate | - |
Helianthus annuus | |
0.354 | - |
glycerol 3-phosphate | pH 7.5, 30°C | Helianthus annuus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Helianthus annuus | - |
- |
microsome | GPAT is prepared from microsomes | Helianthus annuus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60100 | - |
SDS-PAGE | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
high solubilisation is achieved using 0.75% Tween80 and the solubilised GPAT is partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column | Helianthus annuus |
partial | Helianthus annuus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Helianthus annuus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
maximum value for GPAT activity identified between 15 and 20 days after flowering | Helianthus annuus |
0.037 | - |
pH 7.5, 30°C | Helianthus annuus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleoyl-CoA + glycerol 3-phosphate | preferred substrate linoleoyl-CoA (equal to palmitoyl-CoA) | Helianthus annuus | CoA + 1-linoleoyl-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + glycerol 3-phosphate | and palmitoyl-CoA, best substrates | Helianthus annuus | CoA + 1-linoleoyl-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + glycerol 3-phosphate | - |
Helianthus annuus | CoA + 1-oleoyl-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + glycerol 3-phosphate | moderate activity with oleoyl-CoA | Helianthus annuus | CoA + 1-oleoyl-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + glycerol 3-phosphate | preferred substrate palmitoyl-CoA (equal to linoleoyl-CoA) | Helianthus annuus | CoA + 1-palmitoyl-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + glycerol 3-phosphate | and lineoyl-CoA, best substrates | Helianthus annuus | CoA + 1-palmitoyl-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + glycerol 3-phosphate | - |
Helianthus annuus | CoA + 1-stearoyl-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Helianthus annuus |
GPAT | - |
Helianthus annuus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Helianthus annuus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Helianthus annuus |
7.5 | - |
assay at and optimal pH | Helianthus annuus |