Cloned (Comment) | Organism |
---|---|
the PCR product is subcloned with the TOPO TA Cloning Kit and sequenced, the vectors pCR2.1-TOPO and pcDNA3.1/Myc-His+ are used, the xGPAT-myc construct is used for transfection of CHO-K1 cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89100 | - |
estimated molecular mass, verified by SDS-PAGE and Western blot analysis | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Mus musculus | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q14DK4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
3T3-L1 cell | pre- and post-adipocytes | Mus musculus | - |
brown adipose tissue | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
mIMCD3 cell | inner medullary collecting duct 3 cell | Mus musculus | - |
spleen | - |
Mus musculus | - |
white adipose tissue | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
fatty acyl-CoA + glycerol-3-phosphate | - |
Mus musculus | CoA + lysophosphatidic acid | - |
? | |
palmitoyl-CoA + glycerol-3-phosphate | activity assay | Mus musculus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase-like protein 1 | - |
Mus musculus |
xGPAT1 | - |
Mus musculus |
xGPAT1-v1 | - |
Mus musculus |
xGPAT1-v2 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
activity assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Mus musculus |