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Literature summary for 2.3.1.15 extracted from

  • Lewin, T.M.; Schwerbrock, N.M.; Lee, D.P.; Coleman, R.A.
    Identification of a new glycerol-3-phosphate acyltransferase isoenzyme, mtGPAT2, in mitochondria (2004), J. Biol. Chem., 279, 13488-13495.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetone activates isozyme mtGPAT1, no activation of isozyme mtGPAT2 Mus musculus
Polymyxin B activates isozyme mtGPAT1 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
elimination of isozyme mtGPAT1 in knockout mice Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of isozyme mtGPAT1-knockout mice Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 33% inhibition of isozyme mtGPAT1, 70% inhibition of isozyme mtGPAT2 Mus musculus
dihydroxyacetonephosphate competitive inhibition of isozyme mtGPAT2, no inhibition of isozyme mtGPAT1 Mus musculus
lysophosphatidic acid product inhibition, all isozymes Mus musculus
additional information no inhibition by malonyl-CoA, substrate inhibition by acyl-CoAs at concentrations above 0.075 mM Mus musculus
NEM inactivation of isozyme mtGPAT2 and the microsomal isozyme Mus musculus
Polymyxin B inhibitis isozyme mtGPAT2 and the microsomal isozyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
sn-glycerol 3-phosphate pH 7.5, 22°C, microsomal isozyme Mus musculus
0.3
-
sn-glycerol 3-phosphate pH 7.5, 22°C, isozyme mtGPAT1-knockout-mice mitochondria Mus musculus
0.4
-
sn-glycerol 3-phosphate pH 7.5, 22°C, wild-type mitochondria Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane microsomal isozyme Mus musculus 5789
-
mitochondrion 2 isozymes in mitochondria, mitochondrial isozymes mtGPAT1 and mtGPAT2 Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
DTT
-
Mus musculus
Mg2+ best at 4 mM MgCl2 Mus musculus
NaF activates Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Mus musculus enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
3 isozymes
-

Purification (Commentary)

Purification (Comment) Organism
isozymes partially by preparation of subcellular fractions Mus musculus

Reaction

Reaction Comment Organism Reaction ID
an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 1-acyl-sn-glycerol 3-phosphate the active site is composed of homology regions I-IV Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate
-
Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
additional information the mitochondrial and microsomal isozymes show different substrate specificity Mus musculus ?
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
-
Mus musculus CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate preferred substrate of isozyme mtGPAT1, not of isozyme mtGPAT2 Mus musculus CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
glycerol-3-phosphate acyltransferase-1
-
Mus musculus
mtGPAT2
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
isozyme mtGPAT2 is temperature-sensitive Mus musculus
40.5
-
half-life of microsomal isozyme is 7 min, loss of 80% activity of isozyme mtGPAT2 after 4 min Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.545
-
dihydroxyacetonephosphate pH 7.5, 22°C, isozyme mtGPAT2 Mus musculus