Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | activates isozyme mtGPAT1, no activation of isozyme mtGPAT2 | Mus musculus | |
Polymyxin B | activates isozyme mtGPAT1 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
elimination of isozyme mtGPAT1 in knockout mice | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of isozyme mtGPAT1-knockout mice | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 33% inhibition of isozyme mtGPAT1, 70% inhibition of isozyme mtGPAT2 | Mus musculus | |
dihydroxyacetonephosphate | competitive inhibition of isozyme mtGPAT2, no inhibition of isozyme mtGPAT1 | Mus musculus | |
lysophosphatidic acid | product inhibition, all isozymes | Mus musculus | |
additional information | no inhibition by malonyl-CoA, substrate inhibition by acyl-CoAs at concentrations above 0.075 mM | Mus musculus | |
NEM | inactivation of isozyme mtGPAT2 and the microsomal isozyme | Mus musculus | |
Polymyxin B | inhibitis isozyme mtGPAT2 and the microsomal isozyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
sn-glycerol 3-phosphate | pH 7.5, 22°C, microsomal isozyme | Mus musculus | |
0.3 | - |
sn-glycerol 3-phosphate | pH 7.5, 22°C, isozyme mtGPAT1-knockout-mice mitochondria | Mus musculus | |
0.4 | - |
sn-glycerol 3-phosphate | pH 7.5, 22°C, wild-type mitochondria | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | microsomal isozyme | Mus musculus | 5789 | - |
mitochondrion | 2 isozymes in mitochondria, mitochondrial isozymes mtGPAT1 and mtGPAT2 | Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Mus musculus | |
Mg2+ | best at 4 mM MgCl2 | Mus musculus | |
NaF | activates | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Mus musculus | enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
3 isozymes | - |
Purification (Comment) | Organism |
---|---|
isozymes partially by preparation of subcellular fractions | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 1-acyl-sn-glycerol 3-phosphate | the active site is composed of homology regions I-IV | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs | Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the mitochondrial and microsomal isozymes show different substrate specificity | Mus musculus | ? | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | preferred substrate of isozyme mtGPAT1, not of isozyme mtGPAT2 | Mus musculus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase-1 | - |
Mus musculus |
mtGPAT2 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
isozyme mtGPAT2 is temperature-sensitive | Mus musculus |
40.5 | - |
half-life of microsomal isozyme is 7 min, loss of 80% activity of isozyme mtGPAT2 after 4 min | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.545 | - |
dihydroxyacetonephosphate | pH 7.5, 22°C, isozyme mtGPAT2 | Mus musculus |