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Literature summary for 2.3.1.15 extracted from

  • Hayman, M.W.; Fawcett, T.; Schierer, T.F.; Simon, J.W.; Kroon, J.T.M.; Gilroy, J.S.; Rice, D.W.; Rafferty, J.; Turnbull, A.P.; Sedelnikova, S.E.; Slabas, A.R.
    Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity (2000), Biochem. Soc. Trans., 28, 680-681.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
expression of wild-type and PCR-mutated cDNA in Escherichia coli Cucurbita moschata

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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Cucurbita moschata
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleoyl-CoA + sn-glycerol 3-phosphate
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Cucurbita moschata CoA + 1-oleoyl-sn-glycerol 3-phosphate
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?
oleoyl-CoA + sn-glycerol 3-phosphate preferred over palmitoyl-CoA with a ratio of approx. 3:1 Arabidopsis thaliana CoA + 1-oleoyl-sn-glycerol 3-phosphate
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?
palmitoyl-CoA + sn-glycerol 3-phosphate wild-type acyltransferase uses palmitoyl-CoA and oleoyl-CoA at comparable rates, recombinant mutant acyltransferase prefers oleoyl-CoA Cucurbita moschata CoA + 1-palmitoyl-sn-glycerol 3-phosphate
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?