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Literature summary for 2.3.1.15 extracted from

  • Vancura, A.; Haldar, D.
    Purification and characterization of glycerophosphate acyltransferase from rat liver mitochondria (1994), J. Biol. Chem., 269, 27209-27215.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin activation of reconstituted mitochondrial enzyme Rattus norvegicus
Lubrol Px above the critical micellar concentration Rattus norvegicus
phosphatidylcholine i.e. lecithin, activation of mitochondrial enzyme Rattus norvegicus
phosphatidylethanolamine activation of reconstituted mitochondrial acyl transferase Rattus norvegicus
phosphatidylglycerol highest activation of reconstituted mitochondrial acyltransferase in the presence of Lubrol PX Rattus norvegicus
phosphatidylinositol activation of mitochondrial enzyme Rattus norvegicus
phosphatidylserine activation of mitochondrial enzyme Rattus norvegicus
Zwittergent 3-14 above the critical micellar concentration Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid trivial name CHAPS, complete inhibition Rattus norvegicus
cholate complete inhibition Rattus norvegicus
deoxycholate complete inhibition Rattus norvegicus
diacylglycerophosphate
-
Rattus norvegicus
Monoacylglycerophosphate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
palmitoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
0.006
-
palmitoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
0.0064
-
stearoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
0.007
-
palmitoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
0.0084
-
oleoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
0.011
-
stearoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
0.012
-
myristoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
0.013
-
stearoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
0.013
-
oleoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
0.015
-
oleoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
0.016
-
myristoyl-CoA mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus
0.016
-
myristoyl-CoA mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
0.46
-
glycerol 3-phosphate mitochondrial acyl transferase reconstituted in 1,2-dioleoylphosphatidylglycerol Rattus norvegicus
0.82
-
glycerol 3-phosphate mitochondrial acyl transferase reconstituted in asolectin Rattus norvegicus
0.93
-
glycerol 3-phosphate mitochondrial acyl transferase reconstituted in 1,2-dipalmitoylphosphatidylglycerol Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane
-
Rattus norvegicus 5741
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
1 * 85000, mitochondrial acyltransferase, SDS-PAGE Rattus norvegicus
95000 105000 mitochondrial acyltransferase, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300 Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution in asolection or phosphatidylglycerol/phosphatidylethanolamine mixture Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.043
-
mitochondrial acyltransferase reconstituted in asolectin Rattus norvegicus
0.19
-
mitochondrial acyltransferase reconstituted in phosphatidylglycerol/phosphatidylethanolamine mixture Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + sn-glycerol 3-phosphate
-
Rattus norvegicus CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
-
Rattus norvegicus CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
stearoyl-CoA + sn-glycerol 3-phosphate
-
Rattus norvegicus CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 85000, mitochondrial acyltransferase, SDS-PAGE Rattus norvegicus