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Literature summary for 2.3.1.15 extracted from

  • Green, P.R.; Merrill, A.H.; Bell, R.M.
    Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycerol-3-phosphate acyltransferase (1981), J. Biol. Chem., 256, 11151-11159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin alleviates detergent effect of palmitoyl-CoA Escherichia coli
phosphatidylglycerol
-
Escherichia coli
Phospholipids reconstituted activity of Triton X-100-solubilized enzyme, maximal reconstitution at a phospholipid/Triton X-100 ratio of 20/1 Escherichia coli
Phospholipids activation, e.g. phosphatidylglycerol and cardiolipin, i.e. diphosphatidyl glycerol, ratio 2/1, protect against inhibition by palmitoyl-CoA Escherichia coli

General Stability

General Stability Organism
column elution during chromatography causes aggregation leading to irreversible inactivation Escherichia coli
phospholipids reconstitute Triton X-100-solubilized enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration Escherichia coli
Ca2+ in the absence of NaCl Escherichia coli
Mg2+ in the absence of NaCl Escherichia coli
palmitoyl-CoA
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
palmitoyl-CoA
-
Escherichia coli
0.005
-
oleoyl-CoA
-
Escherichia coli
0.015
-
palmitoyl-[acyl carrier protein]
-
Escherichia coli
0.025
-
vaccenoyl-[acyl carrier protein]
-
Escherichia coli
0.15
-
sn-glycerol 3-phosphate acyl donor palmitoyl-CoA or palmitoyl-ACP Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane integral cytoplasmic membrane protein Escherichia coli 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ inhibitory in the absence of NaCl Escherichia coli
KCl activation at 200 mM Escherichia coli
LiCl activation at 200 mM Escherichia coli
Mg2+ inhibitory in the absence of NaCl Escherichia coli
NaCl activation at 200 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid analysis Escherichia coli
83000
-
x * 83000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-

Purification (Commentary)

Purification (Comment) Organism
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite Escherichia coli
Triton X-100-solubilized Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution in dispersed E. coli phospholipids Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3 8
-
Escherichia coli
6.8
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, at least 10 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
?
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + cis-vaccenoyl-sn-glycerol 3-phosphate
-
?
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
?
myristoyl-CoA + sn-glycerol 3-phosphate i.e. tetradecanoyl-CoA Escherichia coli CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
?
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoleoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-[acyl carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli holo-[acyl-carrier protein] + 1-palmitoylglycerol 3-phosphate
-
?
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
stearoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
?
undecanoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-undecanoyl-sn-glycerol 3-phosphate
-
?
vaccenoyl-[acyl carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli holo-[acyl-carrier protein] + 1-vaccenoylglycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 83000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Escherichia coli