Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | alleviates detergent effect of palmitoyl-CoA | Escherichia coli | |
phosphatidylglycerol | - |
Escherichia coli | |
Phospholipids | reconstituted activity of Triton X-100-solubilized enzyme, maximal reconstitution at a phospholipid/Triton X-100 ratio of 20/1 | Escherichia coli | |
Phospholipids | activation, e.g. phosphatidylglycerol and cardiolipin, i.e. diphosphatidyl glycerol, ratio 2/1, protect against inhibition by palmitoyl-CoA | Escherichia coli |
General Stability | Organism |
---|---|
column elution during chromatography causes aggregation leading to irreversible inactivation | Escherichia coli |
phospholipids reconstitute Triton X-100-solubilized enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | above 6 mg/ml, at 0.01 mg palmitoyl-CoA depending on palmitoyl-CoA and phospholipid concentration | Escherichia coli | |
Ca2+ | in the absence of NaCl | Escherichia coli | |
Mg2+ | in the absence of NaCl | Escherichia coli | |
palmitoyl-CoA | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
palmitoyl-CoA | - |
Escherichia coli | |
0.005 | - |
oleoyl-CoA | - |
Escherichia coli | |
0.015 | - |
palmitoyl-[acyl carrier protein] | - |
Escherichia coli | |
0.025 | - |
vaccenoyl-[acyl carrier protein] | - |
Escherichia coli | |
0.15 | - |
sn-glycerol 3-phosphate | acyl donor palmitoyl-CoA or palmitoyl-ACP | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | integral cytoplasmic membrane protein | Escherichia coli | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibitory in the absence of NaCl | Escherichia coli | |
KCl | activation at 200 mM | Escherichia coli | |
LiCl | activation at 200 mM | Escherichia coli | |
Mg2+ | inhibitory in the absence of NaCl | Escherichia coli | |
NaCl | activation at 200 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid analysis | Escherichia coli |
83000 | - |
x * 83000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4 | - |
Purification (Comment) | Organism |
---|---|
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite | Escherichia coli |
Triton X-100-solubilized | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution in dispersed E. coli phospholipids | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | 8 | - |
Escherichia coli |
6.8 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, at least 10 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
myristoyl-CoA + sn-glycerol 3-phosphate | i.e. tetradecanoyl-CoA | Escherichia coli | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
n-dodecanoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoleoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-[acyl carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | holo-[acyl-carrier protein] + 1-palmitoylglycerol 3-phosphate | - |
? | |
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? | |
undecanoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-undecanoyl-sn-glycerol 3-phosphate | - |
? | |
vaccenoyl-[acyl carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | holo-[acyl-carrier protein] + 1-vaccenoylglycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |