Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | activation of mitochondrial enzyme, inhibition of microsomal enzyme | Rattus norvegicus | |
asolectin | i.e. crude mixture of soybean phospholipids, activation of mitochondrial enzyme | Rattus norvegicus | |
Non-site-specific proteases | activation, e.g. proteinase K, subtilisin, not chymotrypsin, trypsin, mitochondrial enzyme, degree of inhibition increases with decreasing ionic strength | Rattus norvegicus | |
phosphatidylcholine | i.e. lecithin, activation of mitochondrial enzyme | Rattus norvegicus | |
phosphatidylethanolamine | activation of microsomal enzyme | Rattus norvegicus | |
phosphatidylserine | activation of mitochondrial enzyme | Rattus norvegicus | |
Polymyxin B | activation of mitochondrial enzyme, inhibition of microsomal enzyme | Rattus norvegicus | |
Proteases | activation, microsomal enzyme | Rattus norvegicus |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Rattus norvegicus |
phospholipids stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | inhibition of mitochondrial enzyme | Rattus norvegicus | |
diacylglycerophosphate | i.e. phosphatidic acid, inhibition of mitochondrial enzyme | Rattus norvegicus | |
iodoacetamide | - |
Rattus norvegicus | |
Monoacylglycerophosphate | i.e. lysophosphatidic acid, inhibition of mitochondrial enzyme | Rattus norvegicus | |
additional information | liver microsomal enzyme is inhibited by all proteases; liver mitochondrial enzyme, not inhibited by trypsin and chymotrypsin | Rattus norvegicus | |
N-ethylmaleimide | strong inhibition of microsomal isozyme, mitochondrial enzyme is not inhibited | Rattus norvegicus | |
phosphatidylinositol | inhibition of microsomal enzyme | Rattus norvegicus | |
phosphatidylserine | inhibition of microsomal enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 0.2 | sn-glycerol 3-phosphate | microsomal enzyme | Rattus norvegicus | |
1 | - |
sn-glycerol 3-phosphate | mitochondrial enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
mitochondrial outer membrane | spans transverse plane of outer membrane | Rattus norvegicus | 5741 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation of microsomal and mitochondrial enzyme | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 85000 | mitochondrial enzyme, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Rattus norvegicus | initial reaction of phosphoglycerol biosynthesis | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
microsomal enzyme, Sepharose 2B, sucrose gradient, partial purification | Rattus norvegicus |
mitochondrial enzyme, Sepharose Q, BioGel HAT, octyl-Sepharose CL-4B, partial purification | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
mitochondrial enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | saturated acyl-CoAs preferred | Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | initial reaction of phosphoglycerol biosynthesis | Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000-85000, mitochondrial enzyme, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 9 | both microsomal and mitochondrial enzymes exhibit a broad optimum | Rattus norvegicus |