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Literature summary for 2.3.1.15 extracted from

  • Fritz, P.K.; Kauffman, J.M.; Robertson, C.A.; Wilson, M.R.
    Cocoa butter biosynthesis. Purification and characterization of a soluble sn-glycerol-3-phosphate acyltransferase from cocoa seeds (1986), J. Biol. Chem., 261, 194-199.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Antibodies to cocoa seed enzyme raised in rabbit Theobroma cacao

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
stearoyl-CoA
-
Theobroma cacao
4.2
-
sn-glycerol 3-phosphate
-
Theobroma cacao

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Theobroma cacao 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
10 * 20000, SDS-PAGE Theobroma cacao
200000
-
gel filtration Theobroma cacao

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Theobroma cacao initial reaction of cocoa butter biosynthesis CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Theobroma cacao
-
cocoa
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, Sephacryl S-300 Theobroma cacao

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Theobroma cacao
-
seed
-
Theobroma cacao
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.69
-
-
Theobroma cacao

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Theobroma cacao CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate initial reaction of cocoa butter biosynthesis Theobroma cacao CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Theobroma cacao CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
stearoyl-CoA + sn-glycerol 3-phosphate
-
Theobroma cacao CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
decamer 10 * 20000, SDS-PAGE Theobroma cacao