Crystallization (Comment) | Organism |
---|---|
homology modeling of enzyme based on the structure of the yeast enzyme.In the model, the S167 and S506 residues lie near the FAD binding site, while the S539 residue is found near the thiamine diphosphate binding site | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
S167A | 73% of wild-type activity | Nicotiana tabacum |
S167F | inactive, mutation abolishes the binding affinity for cofactor FAD. The far-UV spectrum is similar to wild-type | Nicotiana tabacum |
S167R | 250% of wild-type activity | Nicotiana tabacum |
S506A | 230% of wild-type activity | Nicotiana tabacum |
S506F | inactive, mutation abolishes the binding affinity for cofactor FAD. The far-UV spectrum is similar to wild-type | Nicotiana tabacum |
S506R | 183% of wild-type activity | Nicotiana tabacum |
S539A | 73% of wild-type activity, strong resistance to herbicides NC-311, a sulfonylurea, Cadre, an imidazolinone, and a triazolopyrimidine | Nicotiana tabacum |
S539F | 171% of wild-type activity, strong resistance to herbicides NC-311, a sulfonylurea, Cadre, an imidazolinone, and a triazolopyrimidine | Nicotiana tabacum |
S539R | 30% of wild-type activity | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cadre | imidazolinone inhibitor | Nicotiana tabacum | |
NC-311 | sulfonylurea inhibitor | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.38 | - |
pyruvate | mutant S539R, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
3.33 | - |
pyruvate | mutant S539A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
3.36 | - |
pyruvate | mutant S167A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
4.58 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
7.82 | - |
pyruvate | mutant S539F, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
8.36 | - |
pyruvate | mutant S506R, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
10.54 | - |
pyruvate | mutant S506A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | |
11.48 | - |
pyruvate | mutant S167R, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00024 | - |
mutant S167R, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | Cadre | |
0.00089 | - |
mutant S506A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | Cadre | |
0.0026 | - |
mutant S167A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | Cadre | |
0.0035 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | Cadre | |
0.008 | - |
mutant S506A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | NC-311 | |
0.032 | - |
mutant S167A, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | NC-311 | |
0.054 | - |
wild-type, pH not specified in the publication, temperature not specified in the publication | Nicotiana tabacum | NC-311 |