Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Leuconostoc lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 20 mM, no residual activity | Leuconostoc lactis | |
additional information | no effect: Mn2+, Mg2+, Ca2+ | Leuconostoc lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
pyruvate | 30°C, 120 ng protein in assay | Leuconostoc lactis | |
10 | - |
pyruvate | 30°C, 60 ng protein in assay | Leuconostoc lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect: Mn2+, Mg2+, Ca2+ | Leuconostoc lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
PAGE | Leuconostoc lactis |
120000 | - |
PAGE | Leuconostoc lactis |
182000 | - |
PAGE | Leuconostoc lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc lactis | - |
overexpression in Escherichia coli | - |
Leuconostoc lactis NCW1 | - |
overexpression in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity also depends on protein concentration, with lower concentration giving lower activity, activity may be generally increased by pre-incubation at 30°C prior to assay | Leuconostoc lactis |
150 | - |
30°C, 60 ng protein per assay | Leuconostoc lactis |
267 | - |
30°C, 90 ng protein per assay | Leuconostoc lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Leuconostoc lactis | 2-acetolactate + CO2 | - |
? | |
pyruvate | - |
Leuconostoc lactis NCW1 | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE, enzyme exists simultaneously as monomer, dimer and trimer | Leuconostoc lactis |
monomer | 1 * 50000, SDS-PAGE, enzyme exists simultaneously as monomer, dimer and trimer | Leuconostoc lactis |
More | N-terminal sequence analysis | Leuconostoc lactis |
trimer | 3 * 50000, SDS-PAGE, enzyme exists simultaneously as monomer, dimer and trimer | Leuconostoc lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 hour, no loss of activity | Leuconostoc lactis |