Application | Comment | Organism |
---|---|---|
medicine | potential target for antineoplastic chemotherapy | Gallus gallus |
medicine | potential target for antineoplastic chemotherapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21.DE3 as His-tag fusion protein | Gallus gallus |
expressed in Escherichia coli BL21.DE3 as His-tag fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystallized in complex with its inhibitor 2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid | Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site | Gallus gallus | |
Acid Yellow 17 | IC50: 0.012 mM | Gallus gallus | |
NCI324571-C | IC50: 0.042 mM | Gallus gallus | |
NCI324572-F | IC50: 0.012 mM | Gallus gallus | |
NCI3262203-A | IC50: 0.012 mM | Gallus gallus | |
NCI47729-M | IC50: 0.003 mM | Gallus gallus | |
SALOR2 | IC50: 0.0014 mM | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | Gallus gallus | penultimate step of the purine de novo synthesis pathway | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | Homo sapiens | penultimate step of the purine de novo synthesis pathway | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P31335 | - |
- |
Homo sapiens | P31939 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Gallus gallus |
recombinant protein using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | - |
Gallus gallus | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | - |
Homo sapiens | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | penultimate step of the purine de novo synthesis pathway | Gallus gallus | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | penultimate step of the purine de novo synthesis pathway | Homo sapiens | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AICAR transformylase/inosine monophosphate cyclohydrolase | bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway | Gallus gallus |
AICAR transformylase/inosine monophosphate cyclohydrolase | bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Gallus gallus | |
10-formyltetrahydrofolate | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid | pH 7.5 | Gallus gallus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0014 | - |
IC50: 0.0014 mM | Gallus gallus | SALOR2 | |
0.003 | - |
IC50: 0.003 mM | Gallus gallus | NCI47729-M | |
0.012 | - |
IC50: 0.012 mM | Gallus gallus | NCI3262203-A | |
0.012 | - |
IC50: 0.012 mM | Gallus gallus | NCI324572-F | |
0.012 | - |
IC50: 0.012 mM | Gallus gallus | Acid Yellow 17 | |
0.012 | - |
Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site | Gallus gallus | 2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid | |
0.042 | - |
IC50: 0.042 mM | Gallus gallus | NCI324571-C |