Information on EC 2.1.2.3 - phosphoribosylaminoimidazolecarboxamide formyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.1.2.3
-
RECOMMENDED NAME
GeneOntology No.
phosphoribosylaminoimidazolecarboxamide formyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
formyl group transfer
-
-
-
-
formyl group transfer
-
-
formyl group transfer
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
inosine-5'-phosphate biosynthesis I
-
inosine-5'-phosphate biosynthesis II
-
Metabolic pathways
-
One carbon pool by folate
-
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:5'-phosphoribosyl-5-amino-4-imidazole-carboxamide N-formyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10-formyltetrahydrofolate:5'-phosphoribosyl-5-amino-4-imidazolecarboxamide formyltransferase
-
-
-
-
5'-phosphoribosyl-5-amino-4-imidazolecarboxamide formyltransferase
-
-
-
-
5-amino-1-ribosyl-4-imidazolecarboxamide 5'-phosphate transformylase
-
-
-
-
5-amino-4-imidazolecarboxamide ribonucleotide transformylase
-
-
-
-
5-amino-4-imidazolecarboxamide ribotide formyltransferase
-
-
5-amino-4-imidazolecarboxamide ribotide transformylase
-
-
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase (AICAR Tfase)-inosine monophosphate cyclohydrolase
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase
-
-
5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase
-
-
5-aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase
-
bifunctional enzyme
AICA-ribotide formyltransferase
-
-
AICAR formyltransferase
-
-
-
-
AICAR formyltransferase
-
-
AICAR formyltransferase
-
-
AICAR TFase
-
-
AICAR TFase
-
-
AICAR TFase
-
-
AICAR transformylase
-
-
-
-
AICAR transformylase
-
-
AICAR transformylase
-
-
AICAR transformylase
-
-
AICAR transformylase
-
-
AICAR transformylase/inosine monophosphate cyclohydrolase
-
bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway
AICAR transformylase/inosine monophosphate cyclohydrolase
-
bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway
AICAR transformylase/inosine monophosphate cyclohydrolase
-
bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway
AICARFT/IMPCHase
-
-
aminoimidazole-4-carboxamide ribonucleotide transformylase
-
-
aminoimidazolecarboxamide ribonucleotide transformylase
-
-
-
-
aminoimidazolecarboxamide ribotide transformylase
-
-
ATIC
-
-
IMPCH
-
-
CAS REGISTRY NUMBER
COMMENTARY
9032-03-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
onion
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
gene purH
-
-
Manually annotated by BRENDA team
wild-type strain 5928, strain PA 15
-
-
Manually annotated by BRENDA team
avian; chicken
-
-
Manually annotated by BRENDA team
bifunctional 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
SwissProt
Manually annotated by BRENDA team
human
SwissProt
Manually annotated by BRENDA team
mouse, strain MRL/lpr
-
-
Manually annotated by BRENDA team
pea, L. var. Alaska
-
-
Manually annotated by BRENDA team
Trifolium sp.
trefoil
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
aminoimidazolecarboxamide ribotide transformylase is involved in the de novo purine nucleotide biosynthesis. No enzyme complex that generates 10-formyl-5,6,7,8-tetrahydrofolate and immediately channels or furnishes it to AICAR transformylase is needed because the first oxidation product of 10-formyl-5,6,7,8-tetrahydrofolate is 10-formyl-7,8-dihydrofolate that is utilized by this transformylase
physiological function
-
10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase, an enzyme in purine nucleotide biosynthesis de novo, which introduces C2 into the purine ring
metabolism
-
aminoimidazolecarboxamide ribotide transformylase is involved in the de novo purine nucleotide biosynthesis
additional information
-
the enzyme is located at the C-terminus of the bifunctional purine-biosynthesis protein, PurH, whose N-terminus possesses IMP cyclohydrolase activity. Coupling of the two domains is essential for the catalytic process, as the AICAR Tfase reaction favours the reverse direction by itself and the irreversible cyclization of 5-formyl-aminoimidazole-4-carboxamide ribonucleotide to IMP drives formyl transfer in the forward direction
additional information
-
structure and active site of AICAR transformylase are not consistent with other enzymes that utilize 10-formyl-5,6,7,8-tetrahydrofolate. Methotrexate blockage of the AICAR transformylase process in patients with rheumatoid arthritis suggests that dihydrofolate reductase is involved and is consistent with dihydrofolate and 10-formyl-7,8-dihydrofolate being the product and substrate for AICAR transformylase
additional information
-
the enzyme is part of the the bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase, ATIC, or PurH
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(6R,6S)-10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
P31939
-
-
r
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase
-
-
r
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
10-formyl-7,8-dihydrofolate is kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylas
-
-
r
10-formyl-7,8-dihydropteroylpenta-gamma-glutamate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide
7,8-dihydropteroylpenta-gamma-glutarate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
?
10-formyl-8-deazafolate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide
8-deazafolate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
?
10-formyl-tetrahydrofolic acid + 5'-phosphoribosyl-5-amino-imidazole-carboxamide
5,6,7,8-tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyldihydrofolic acid pentaglutamate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide
dihydrofolic acid pentaglutamate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31335
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31939
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31335
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31939
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
Columba sp., Trifolium sp.
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
precursors for inosinic acid biosynthesis
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
P31939
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
reduced folate cofactor requiring step of de novo purine biosynthesis
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
reduced folate cofactor requiring step of de novo purine biosynthesis
-
-
r
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
P31939
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
Columba sp., Trifolium sp.
-
-
-
r
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide
show the reaction diagram
-
-
-
-
?
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroylheptaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroylhexaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroylmonoglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylmonoglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroylpentaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroyltetraglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N10-formyltetrahydropteroyltriglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotide
tetrahydorpteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
?
N5,N10-anhydroformyltetrahydrofolic acid + glycinamide ribonucleotide
N10-formyltetrahydrofolic acid + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
r
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
show the reaction diagram
-
-
-
r
10-formyltetrahydrofolate + 5-amino-imidazole-4-thiocarboxamide ribonucleotide
tetrahydrofolate + 6-mercaptopurine ribonucleotide
show the reaction diagram
-
-
-
?
additional information
?
-
-
no activity with N5,N10-methenyl analogues
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
P31939
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
Columba sp., Trifolium sp.
-
in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene
-
-
-
additional information
?
-
-
polymorphisms in 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene is not associated with recurrent venous thrombosis risk
-
-
-
additional information
?
-
-
10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31335
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
P31939
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram
-
penultimate step of the purine de novo synthesis pathway
-
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
Columba sp., Trifolium sp.
-
-
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
precursors for inosinic acid biosynthesis
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
P31939
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
penultimate and final steps in the de novo purine biosynthesis pathway
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
de novo synthesis of purine nucleotides
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
reduced folate cofactor requiring step of de novo purine biosynthesis
-
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram
-
reduced folate cofactor requiring step of de novo purine biosynthesis
-
-
r
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
tetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
polymorphisms in 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene is not associated with recurrent venous thrombosis risk
-
-
-
additional information
?
-
-
10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
10-formyldihydrofolate
-
-
10-formylfolate
-
-
10-formyltetrahydrofolate
-
-
10-formyltetrahydrofolate
P31335
-
N10-formyl-7,8-dihydrofolate
-
kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylase
N10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
-
10-formyltetrahydrofolate
-
-
additional information
-
contains selenomethionine
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
K+
-
essential component
K+
-
required for maximum enzymatic activity
K+
-
the refined structure includes two K+ ions in the asymmetric unit
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2RS)-N-[4-{1-(acetamideoxy)-3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{1-(acetoxy)-3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1,1-(dimethoxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(1,1-bromoacetoxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(1,1-dimethoxyacetoxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(ethoxalyoxy) prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(formyloxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(hydroxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(pyruvoyloxy)prop-2-yl}benzoyl]-L-glutamic acid
-
-
(6S/R)-5,10-dideaza-5,6,7,8,-tetrahydrofolate
-
-
10-formyl-5,6,7,8-tetrahydropteroylpenta-gamma-glutamate
-
-
10-formyl-5,8,10-trideazafolate
-
-
10-formylfolic acid pentaglutamate
-
competitive inhibitor
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid
-
Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site
2-[[([4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
2-[[([4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
2-[[([4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorophenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
2-[[([4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
-
2-[[([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,5-dihydrothiophen-2-yl]carbonyl)amino]methyl]-3-methylidenebutanedioic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
4-acetamidophenol
-
-
4-Amino-10-methylpteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
methotrexate polyglutamate, competitive inhibitor
4-amino-10-methylpteroylglutamic acid
-
methotrexate, non-competitive inhibitor
4-amino-10-methylpteroylglutamic acid
-
methotrexate, non-competitive inhibitor
4-amino-4-deoxy-5,8,10-trideazapteroyl-D,L-40-methyleneglutamic acid
-
weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase
4-N-allyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
4-N-methyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
product inhibitor
5-amino-1-beta-D-ribofuranosylimidazole 5'-phosphate
-
-
5-amino-1-beta-D-ribofuranosylimidazole-4-carbonitrile 5'-phosphate
-
-
5-amino-1-beta-D-ribofuranosylimidazole-4-carboxamidoxime 5'-phosphate
-
-
5-amino-1-beta-D-ribofuranosylimidazole-4-carboxylate 5'-phosphate
-
-
5-amino-4-nitro-1-beta-D-ribofuranosylimidazole 5'-phosphate
-
-
5-amino-4-nitroimidazole ribonucleotide
-
-
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
7,8-dihydropteroylpenta-gamma-glutamate
-
-
Acid Yellow 17
-
IC50: 0.012 mM
Azathioprine
-
competitive inhibitor
Azathioprine
-
-
beta-DADF
-
IC50 125 nM
BW1540
-
sulfamido-bridged 5,8-dideazafolate analogue
BW2315
-
sulfamido-bridged 5,8-dideazafolate analogue
Cappsin 1
-
non-competitive, inhibition of activity by preventing the formation of dimers
-
Cappsin 2
-
some derivatives of Cappepsin are also inhibitory, inhibition of activity by preventing the formation of dimers
-
dihydrofolic acid pentaglutamate
-
-
dihydrofolic acid pentaglutamate
-
-
folic acid pentaglutamate
-
competitive inhibitor
-
Ibuprofen
-
anti-inflammatory drug
methotrexate
-
-
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
-
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N10-formyltetrahydropteroylglutamate
-
-
Naproxen
-
anti-inflammatory drug
NCI324571-C
-
IC50: 0.042 mM
NCI324572-F
-
IC50: 0.012 mM
NCI3262203-A
-
IC50: 0.012 mM
NCI47729-M
-
IC50: 0.003 mM
NSC126445
-
IC50: 0.010mM
NSC170645
-
IC50: 0.036 mM
NSC26699
-
IC50: 0.017 mM
NSC292213
-
IC50: 0.009 mM
NSC30171
-
IC50: 0.0006 mM
NSC321237
-
IC50: 0.106 mM
NSC324571
-
IC50: 0.020 mM
NSC324572
-
IC50: 0.012 mM
NSC324981
-
IC50: 0.020 mM
NSC326203
-
IC50: 0.012 mM
NSC326211
-
IC50: 0.204 mM
NSC37031
-
IC50: 0.008 mM
NSC37173
-
IC50: 0.004 mM
NSC41806
-
IC50: 0.055 mM
NSC45592
-
IC50: 0.012 mM
NSC47729
-
IC50: 0.003 mM
NSC58046
-
IC50: 0.008 mM
NSC7524
-
IC50: 0.231 mM
NSC88915
-
IC50: 0.014 mM
paracetamol
-
-
pteroylpenta-gamma-glutamate
-
-
SALOR2
-
IC50: 0.0014 mM
sulphalazine
-
-
thioinosinic acid
-
competitive inhibitor
thioinosinic acid
-
-
tylenol
-
-
methotrexate
P31939
enzyme phosphorylation dampens the methotrexate-mediated transformylase activity inhibition
additional information
-
specific antifolate reagents and nonfolate inhibitors are analogues of cofactor N10-formyltetrahydrofolate and can completely inhibit AICAR Tfase activity
-
additional information
-
methotrexate, MTX, cannot directly inhibit AICAR transformylase, but blocks the AICAR transformylase process in patients with rheumatoid arthritis
-
additional information
-
an adventitiously active site-bound nucleotide, found in the crystal structures, is identified by NMR and mass spectral analysis as a novel 5-formyl derivative of an earlier intermediate in the biosynthetic pathway 4-carboxy-5-aminoimidazole ribonucleotide. The nucleotide is a cyclohydrolase inhibitor. Binding structure analysis, structure modeling, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
P31939
enzyme phosphorylation by anaplastic lymphoma kinase enhances enzymatic activity and dampens the methotrexate-mediated transformylase activity inhibition
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0602
-
(6R,6S)-10-formyltetrahydrofolic acid
P31939
-
0.00051
-
10-formyldihydrofolic acid pentaglutamate
-
-
0.0043
-
10-formyltetrahydrofolate
-
mutant K266R
0.0076
-
10-formyltetrahydrofolate
-
wild-type
0.01
-
10-formyltetrahydrofolate
-
-
0.0679
-
10-formyltetrahydrofolate
-
-
0.2
-
10-formyltetrahydrofolate
-
-
0.031
-
5'-phosphoribosyl-5-amino-imidazolecarboxamide
-
-
0.0009
-
5-amino-imidazole-4-thiocarboxamide ribonucleotide
-
10-formyldihydrofolate as cofactor
0.0005
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
mutant K266R
0.0015
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
10-formyldihydrofolate as cofactor
0.0019
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
10-formyltetrahydrofolate as cofactor
0.0019
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
wild-type
0.01
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
-
0.0168
-
5-aminoimidazole-4-carboxamide ribonucleotide
P31939
-
0.00183
-
N10-formyltetrahydropteroylheptaglutamate
-
25 mM KCl
0.00313
-
N10-formyltetrahydropteroylheptaglutamate
-
150 mM KCl
0.00164
-
N10-formyltetrahydropteroylhexaglutamate
-
25 mM KCl
0.00195
-
N10-formyltetrahydropteroylhexaglutamate
-
150 mM KCl
0.00165
-
N10-formyltetrahydropteroylmonoglutamate
-
25 mM KCl
0.353
-
N10-formyltetrahydropteroylmonoglutamate
-
150 mM KCl
0.00206
-
N10-formyltetrahydropteroylpentaglutamate
-
150 mM KCl
0.00256
-
N10-formyltetrahydropteroylpentaglutamate
-
25 mM KCl
0.00099
-
N10-formyltetrahydropteroyltetraglutamate
-
25 mM KCl
0.00107
-
N10-formyltetrahydropteroyltetraglutamate
-
150 mM KCl
0.00595
-
N10-formyltetrahydropteroyltriglutamate
-
150 mM KCl
0.674
-
N10-formyltetrahydropteroyltriglutamate
-
25 mM KCl
0.185
-
tetrahydrofolate
-
-
0.00023
-
tetrahydrofolic acid
-
-
0.0168
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
-
additional information
-
additional information
-
10-formyl-7,8-dihydrofolate has an about 5fold kinetic advantage, Vm/Km, over 10-formyl-5,6,7,8-tetrahydrofolate for AICAR transformylases in human leukemia cells and for human recombinant AICAR transformylase
-
additional information
-
additional information
-
10-formyl-7,8-dihydrofolate has an about 5fold kinetic advantage, Vm/Km, over 10-formyl-5,6,7,8-tetrahydrofolate for AICAR transformylases in rat bone marrow cells
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00137
-
10-formylfolic acid pentaglutamate
-
-
0.007
-
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid
-
pH 7.5
0.006
-
5-amino-1-beta-D-ribofuranosylimidazole 5'-phosphate
-
-
0.026
-
5-amino-1-beta-D-ribofuranosylimidazole-4-carbonitrile 5'-phosphate
-
-
0.01
-
5-amino-1-beta-D-ribofuranosylimidazole-4-carboxylate 5'-phosphate
-
-
0.0007
-
5-amino-4-nitro-1-beta-D-ribofuranosylimidazole 5'-phosphate
-
-
0.00004
-
5-amino-4-nitroimidazole ribonucleotide
-
-
0.0007
-
5-amino-4-nitroimidazole ribonucleotide
-
-
0.00007
-
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
0.0004
-
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotide
-
-
0.027
-
AMP
-
-
0.09
-
Azathioprine
-
-
0.12
-
Azathioprine
-
-
0.000008
-
BW1540
-
-
0.000006
-
BW2315
-
-
0.003
-
Cappsin 1
-
-
-
0.00014
-
dihydrofolic acid pentaglutamate
-
-
0.00008
-
folic acid pentaglutamate
-
-
-
0.075
-
methotrexate
-
-
0.00315
-
methotrexate polyglutamate
-
-
-
0.000015
-
NSC30171
-
-
0.039
-
thioinosinic acid
-
-
0.11
-
thioinosinic acid
-
-
0.0012
-
XMP
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.012
-
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid
-
Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site
0.012
-
Acid Yellow 17
-
IC50: 0.012 mM
0.042
-
NCI324571-C
-
IC50: 0.042 mM
0.012
-
NCI324572-F
-
IC50: 0.012 mM
0.012
-
NCI3262203-A
-
IC50: 0.012 mM
0.003
-
NCI47729-M
-
IC50: 0.003 mM
0.01
-
NSC126445
-
IC50: 0.010mM
0.036
-
NSC170645
-
IC50: 0.036 mM
0.017
-
NSC26699
-
IC50: 0.017 mM
0.009
-
NSC292213
-
IC50: 0.009 mM
0.0006
-
NSC30171
-
IC50: 0.0006 mM
0.106
-
NSC321237
-
IC50: 0.106 mM
0.02
-
NSC324571
-
IC50: 0.020 mM
0.012
-
NSC324572
-
IC50: 0.012 mM
0.02
-
NSC324981
-
IC50: 0.020 mM
0.012
-
NSC326203
-
IC50: 0.012 mM
0.204
-
NSC326211
-
IC50: 0.204 mM
0.008
-
NSC37031
-
IC50: 0.008 mM
0.004
-
NSC37173
-
IC50: 0.004 mM
0.055
-
NSC41806
-
IC50: 0.055 mM
0.012
-
NSC45592
-
IC50: 0.012 mM
0.003
-
NSC47729
-
IC50: 0.003 mM
0.008
-
NSC58046
-
IC50: 0.008 mM
0.231
-
NSC7524
-
IC50: 0.231 mM
0.014
-
NSC88915
-
IC50: 0.014 mM
0.0014
-
SALOR2
-
IC50: 0.0014 mM
0.000125
-
beta-DADF
-
IC50 125 nM
additional information
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
value above 0.05
additional information
-
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00067
-
-
-
0.0371
-
-
-
0.856
-
-
-
1.19
-
P31939
-
additional information
-
-
1 unit = removal of 0.0001 mM of AICA-ribotide/0.5 ml/20 min at 37°C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10.5
-
-
6.5
8.5
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.49
-
-
calculated isoelectric point
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
AICAR transformylase does not form clusters
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
49700
-
-
monomer, residues 1-452
64430
-
P31939
cDNA sequencing
64450
-
P31939
electrospray mass spectroscopy
65000
-
-
recombinant enzyme, SDS-PAGE
65630
-
P31939
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 64400, deduced from nucleotide sequence of cDNA
dimer
-
crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities
dimer
-
crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities
dimer
-
monomer-dimer equilibrium in solution, dimeric form is required for transformylase activity
dimer
-
transformylase activity is formed upon dimerization of the enzyme
monomer
P31939
1 * 64425, calculated from amino acid sequence
monomer
-
-
additional information
-
PurH is composed of two domains linked by a flexible region. The N-terminal domain possesses IMPCH activity and the C-terminal domain possesses AICAR Tfase activity
additional information
-
the protein is folded into two domains, the N-terminal domain, residues 1-212, containing the cyclohydrolase active site and the C-terminal domain, residues 222-523, containing the formyltransferase active site, structure modeling, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
P31939
bifunctional 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase associates with nucleoplasmin-anaplastic lymphoma kinase, and its phosphorylation requires anaplastic lymphoma kinase activity. Phosphorylation enhances enzymatic activity and dampens the methotrexate-mediated transformylase activity inhibition
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified recombinant His6-tagged PurH, without methylation of the 28 lysine residues, sitting drop vapour diffusion method, mixing of 0.001 ml of 56 mg/ml protein in 0.8 M sodium/potassium hydrogen phosphate, pH 7.5, with 0.001 ml of reservoir solution containing 0.1 M sodium acetate, pH 5.0, 1 M ammonium sulfate, 1 week to 1 month, X-ray diffraction structure determination and analysis at 3.05 A resolution
-
crystallized in complex with its inhibitor 2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acid
-
recombinant His-tag fusion protein crystallized by sitting drop vapor diffusion method in complex with its inhibitor beta-DADF
-
ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2°
-
ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2°; crystallized in a binary complex with methotrexate, single crystal at 96 K to 2.3 A resolution, monoclinic crystals, space group P2(1), with unit-cell dimensions a = 65.17, B = 105.93, c= 103.47 A, beta = 108.27 degrees
-
sitting drop vapor diffusion method, co-crystallized in the presence of substrate with BW1540 and BW2315
-
purified recombinant His-tagged enzyme free or in complex with substrate 5-aminoimidazole-4-carboxamide ribonucleotide, both with adventitiously active site-bound nucleotide inhibitor of the cyclohydrolase activity, X-ray diffraction structure determination and analysis at 2.5 A and 2.2 A resolution, respectively
-
the three-dimensional structure of TM1249 is determined to 1.88 A resolution by molecular replacement
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
much less stable during storage in maleate buffer than in Tris or phosphate solutions
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2°C, if the solution of purified enzyme are stored for 3 or 4 months, a requirement for a specific formyl donor can then be shown
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant N-terminally His6-tagged PurH from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
-
recombinant protein using His-tag
-
inosinicase activity copurifies
-
partially
-
recombinant avian ATIC, purified from E. coli expression system
-
recombinant protein using His-tag
-
recombinant N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
using a nickel-chelating resin and a Resource Q column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cloned and expressed
-
gene purH, expression of N-terminally His6-tagged PurH in Escherichia coli strain Rosetta (DE3)
-
expressed in Escherichia coli as His-tag fusion protein
-
expressed in Escherichia coli BL21.DE3 as His-tag fusion protein
-
ATICcDNA cloned into a pET28a vector and transformed into Escherichia coli BL21.DE3 for overexpression
-
purH gene cloning and sequencing of cDNA
-
cloned from a hepatoma cDNA library; cloned from a placenta cDNA library, overproduced in Escherichia coli
-
cloned from a placenta cDNA library, overproduced in Escherichia coli
-
expressed in Escherichia coli BL21.DE3 as His-tag fusion protein
-
native and mutant proteins
-
purH gene, cloning and sequencing of cDNA, expressed in Escherichia coli
P31939
expression of N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3)
-
into the plasmid pMH4 for expression in the Escherichia coli strain GeneHogs
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
T116S
-
AICAR transformylase/inosine monophosphate cyclohydrolase 346C>G polymorphism
additional information
-
several site directed mutants created with mutations in the inosine monophosphate cyclohydrolase domain of the enzyme
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
potential target for antineoplastic chemotherapy
medicine
-
attractive anticancer target
medicine
-
-
medicine
-
target for development of inhibitors with potential use as chemotherapeutic agents
medicine
-
potential target for development of antineoplastic drugs; target for development of inhibitors with potential use as chemotherapeutic agents
medicine
-
potential target for antineoplastic chemotherapy
medicine
-
potential target for antineoplastic drug design
medicine
-
animal model of systemic autoimmune disease