Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) Rosetta 2 | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
strain HB8 | - |
Purification (Comment) | Organism |
---|---|
partially purified by heat treatment and DE52 column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrmB | - |
Thermus thermophilus |
TrMet (m7G46) | - |
Thermus thermophilus |
tRNA (m7G46) methyltransferase | - |
Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
malfunction | protein synthesis in DELTAtrmB cells is depressed above 70°C. At 80°C, the DtrmB strain exhibits a severe growth defect | Thermus thermophilus |
physiological function | the m7G46 modification catalyzed by TrmB may act as one of the key factors in tRNA modification network | Thermus thermophilus |
physiological function | the m7G46 modification is required for cell viability at high temperatures via a tRNA modification network. The m7G46 modification induces nearly full modification of several modified nucleotides such as Gm18 and m1G37 | Thermus thermophilus |