Information on EC 2.1.1.33 - tRNA (guanine46-N7)-methyltransferase

New: Word Map on EC 2.1.1.33
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.1.1.33
-
RECOMMENDED NAME
GeneOntology No.
tRNA (guanine46-N7)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase
The enzyme specifically methylates guanine46 at N7 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-00-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain MRE 600, 2 isoenzymes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain D84
-
-
Manually annotated by BRENDA team
strain HB8
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
show the reaction diagram
additional information
additional information
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates
Cations
-
mono- and divalent cations activate
K+
contains three potassium ions per asymmetric unit, one in the interface between monomers and one in a region involved in AdoMet binding in the homologous catechol O-MTase, bound to the main chain carbonyls of residues Asn115 and Gly46
Mn2+
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methylthioadenosine
-
competitive inhibitor
Methylthioinosine
-
weak competitive inhibitor, above 0.5 mM
p-hydroxymercuribenzoate
-
-
S-Adenosyl-D-homocysteine
-
competitive inhibitor, L-enantiomer more potent than D-enantiomer by 2 orders of magnitude
S-adenosyl-DL-homocysteine
-
competitive inhibitor
S-adenosyl-L-cysteine
-
competitive inhibitor
S-adenosyl-L-homocysteine
S-Inosylhomocysteine
-
weak competitive inhibitor, above 0.5 mM
Spleen phosphodiesterase
-
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activates
Methylthioinosine
-
activates at 0.05-0.2 mM
S-Inosylhomocysteine
-
activates at 0.05-0.2 mM
additional information
-
not activated by spermine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025 - 0.0037
guanine46 in tRNA
0.21
methyl deficient tRNA from E. coli
-
-
-
0.016 - 0.053
S-adenosyl-L-methionine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.95
-
tRNAfMet from Bacillus subtilis
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
dimethylglutarate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
organelle-specific enzyme, not in cytoplasm
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
glycerol gradient centrifugation
100000
-
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
300000
-
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27307, calculated from sequence
heterodimer
-
the enzyme contains two protein subunits: TRm8 and TRm82
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by hanging drop method, at a resolution of 2.1 A, Rossmann-fold methyltransferase structure with the N-terminal helix folded on the opposite site of the catalytic domain
a truncated protein lacking the first 32 residues of the N-terminus but with an additional His6 tag at the C-terminus is crystallized by hanging-drop vapour-diffusion method using 15% (w/v) PEG 3350 and 150 mM MgSO4, at 10C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without antifreeze agents inactivates, 30% ethylene glycol at -20C stabilizes
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethylene glycol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, frozen in liquid nitrogen, at least several months, stable
-
0C, partially purified, 48 h, 15% loss of activity
-
0C, purified, 6 months, about 70% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1000fold purification by affinity chromatography on Escherichia coli tRNA Sepharose
-
copurification of 2 proteins, Trm8 and Trm82
-
Ni-NTA column chromatography, Sephadex G-25 gel filtration, ammonium sulfate precipitation, and DE52 column chromatography
-
partial purification by phosphocellulose column
-
partially purified by heat treatment and DE52 column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression of both METTL1 and WDR4 in Saccharomyces cerevisiae
-
expressed in a wheat germ cell-free translation system, active Trm8-Trm82 heterodimer is only synthesized under conditions, in which both Trm8 and Trm82 mRNAs are cotranslated
-
expressed in Escherichia coli strain BL21 (DE3) Rosetta 2
-
expression in Escherichia coli
ORF YDL201w encodes Trm8 and ORF YDR165w encodes Trm82, both proteins form a complex with m7G-methyltransferase activity, Trm8 contains a binding domain for S-adenosyl-L-methionine: GXGXG, coexpression of Trm8/Trm82 in Escherichia coli BL21 Codon Plus (DE3) as His6-fusion proteins under control of the Ptac-promoter
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glu202Stop
is unstable to heat treatment as compared to the full-length wild-type enzyme, but has an enzymatic activity
additional information
Show AA Sequence (10385 entries)
Longer loading times are possible. Please use the Sequence Search for a certain query.