Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | Q8CWX6 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), gel fitration | Streptococcus mutans |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
cobalamin-independent methionine synthase (MetE) | - |
Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
0.155 micromol/min/mg, at pH 6.0: 0.152 micromol/min/mg, at pH 7.0: 0.148 micromol/min/mg, at pH 4.6: 0.0096 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg | Streptococcus mutans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.6 | 9 | at pH 4.6: 0.0096 micromol/min/mg, at pH 6.5: 0.155 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg | Streptococcus mutans |