Information on EC 2.1.1.14 - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.1.1.14
-
RECOMMENDED NAME
GeneOntology No.
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
mechanism
-
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
final step in methionine synthesis
-
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
final step in methionine synthesis
-
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
S-methylation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Cysteine and methionine metabolism
-
Metabolic pathways
-
methionine biosynthesis I
-
methionine biosynthesis II
-
methionine biosynthesis III
-
S-adenosyl-L-methionine cycle I
-
S-adenosyl-L-methionine cycle II
-
seleno-amino acid biosynthesis
-
Selenocompound metabolism
-
SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
O50008
-
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
P25665
-
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
P05694
-
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
Q9X112
-
cobalamin-independent methionine synthase
-
-
cobalamin-independent methionine synthase
O50008
-
cobalamin-independent methionine synthase
-
-
cobalamin-independent methionine synthase
-
-
cobalamin-independent methionine synthase
P25665
-
cobalamin-independent methionine synthase
Escherichia coli pJG816
-
-
-
cobalamin-independent methionine synthase
B2VQE0
-
cobalamin-independent methionine synthase
-
-
cobalamin-independent methionine synthase
P05694
-
cobalamin-independent methionine synthase
-
-
cobalamin-independent methionine synthase
Q9X112
-
cobalamin-independent methionine synthase (MetE)
Q8CWX6
-
homocysteine methylase
-
-
-
-
MET1
B2VQE0
-
MetE
P25665
-
MetE
Escherichia coli pJG816
-
-
-
MetE
Q9X112
-
methionine synthase MetE
-
-
methyltetrahydropteroylpolyglutamate:homocysteine methyltransferase
-
-
-
-
methyltransferase, tetrahydropteroylglutamate-homocysteine transmethylase
-
-
-
-
PpMetE
Q6GYJ7
-
tetrahydropteroyltriglutamate methyltransferase
-
-
-
-
TM1286
O50008
-
TM1286
P25665
-
TM1286
Q9X112
-
CAS REGISTRY NUMBER
COMMENTARY
9068-29-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
carrot
-
-
Manually annotated by BRENDA team
strain pJG816
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
-
-
Manually annotated by BRENDA team
Escherichia coli pJG816
strain pJG816
-
-
Manually annotated by BRENDA team
barley
-
-
Manually annotated by BRENDA team
Hemp broomrape
B2VQE0
UniProt
Manually annotated by BRENDA team
Phaseolus sp.
bean
-
-
Manually annotated by BRENDA team
green string bean
-
-
Manually annotated by BRENDA team
pea, 2 enzyme forms: a 5-methyltetrahydropteroylglutamate dependent enzyme and a S-methylmethionine dependent enzyme
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?, r
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?, r
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
Phaseolus sp.
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
Q9X112, -
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
O50008
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
P25665
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
P05694
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
Escherichia coli pJG816
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
Escherichia coli K12
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyl-tri-L-glutamate + L-homocysteine
tetrahydropteroyl-tri-L-glutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroylmonoglutamate + L-homocysteine
tetrahydropteroylmonoglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroylmonoglutamate + L-homocysteine
tetrahydropteroylmonoglutamate + L-methionine
show the reaction diagram
-
-
-
-
-
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
tetrahydropteroylmonoglutamate + L-methionine
show the reaction diagram
-
-
-
-
-
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
tetrahydropteroylmonoglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
S-adenosylmethionine + homocysteine
methionine + S-adenosylhomocysteine
show the reaction diagram
-
-
-
?
S-adenosylmethionine + L-selenohomocysteine
L-selenomethionine + S-adenosylhomocysteine
show the reaction diagram
-
-
reaction catalyzed both by vitamin B12 dependent and independent enzyme
?
S-methylmethionine + homocysteine
methionine
show the reaction diagram
-
-
-
?
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
monoglutamate analog not used
-
-
-
additional information
?
-
-
monoglutamate analog not used
-
-
-
additional information
?
-
-
monoglutamate analog not used
-
-
-
additional information
?
-
-
5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor
-
-
-
additional information
?
-
-
enzymes of higher plants can use the monoglutamate
-
-
-
additional information
?
-
-
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
-
-
-
additional information
?
-
-
final step in methionine synthesis
-
-
-
additional information
?
-
-
final step in methionine synthesis
-
-
-
additional information
?
-
-
does not use 5-methyltetrahydropteroylglutamate as a substrate
-
-
-
additional information
?
-
Escherichia coli K12
-
5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
final step in methionine synthesis
-
-
-
additional information
?
-
-
final step in methionine synthesis
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
-
additional information
-
vitamin-B12 independent enzyme
-
additional information
-
vitamin-B12 independent enzyme
-
additional information
-
no requirement for phosphate or magnesium; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
-
additional information
-
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
-
additional information
-
vitamin-B12 independent enzyme
-
additional information
-
vitamin B12 independent enzyme
-
additional information
-
vitamin-B12 independent enzyme
-
additional information
O50008
vitamin-B12 independent enzyme
-
additional information
P25665
vitamin B12-independent enzyme
-
additional information
-
vitamin-B12 independent enzyme
-
additional information
Q9X112
vitamin B12-independent enzyme
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
required
Mg2+
-
at low phosphate concentration, 0.1 mM, the Mg2+-stimulation is 4.5fold, smaller stimulation at higher concentrations of phosphate; stimulates
Mg2+
-
no stimulation
Mg2+
-
no stimulation
Mn2+
-
below 1 mM more effective than Mg2+, above 1 mM inhibitory
phosphate
-
-
Zinc
-, Q6GYJ7
enzyme contains zinc
Zinc
-
required for activation and binding of L-homocysteine
Zn2+
-
characterization of zinc site
Zn2+
-
1 equivalent per subunit
Zn2+
O50008
requires zinc for activity
Zn2+
P25665
requires zinc for activity
Zn2+
P05694
requires zinc for activity
Zn2+
Q9X112
requires zinc for activity
Zn2+
Q8CWX6, -
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5-methyltetrahydropteroyl-alpha-glutamate
-
-
Chloromethyl ketones
-
-
EDTA
-
in absence of Mg2+
GSSG
-
inactivates by glutathionylation at Cys645
High ionic strength
-
-
-
iodoacetamide
-
-
Mn2+
-
above 1 mM
Pteroyl-alpha-glutamylglutamic acid
-
-
Pteroyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
Zinc
-
His647, Cys649 and Cys733 are involved in zinc coordination. Addition of L-homocysteine leads to reorganization of the zinc binding site with a stronger coordination to Cys649, Cys733, and a water molecule
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.094
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.12
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.028
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
-
0.08
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
-
0.084
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.129
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.109
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.113
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.000026
-
5-methyltetrahydropteroylglutamate
-
-
0.35
-
5-methyltetrahydropteroylmonoglutamate
-
-
0.0044
-
5-methyltetrahydropteroyltriglutamate
-
-
0.4
-
5-methyltetrahydropteroyltriglutamate
-
-
0.041
-
homocysteine
Phaseolus sp.
-
cosubstrate S-methyl-L-methionine
0.069
-
homocysteine
-
-
0.6
-
homocysteine
-
cosubstrate 5-methyltetrahydropteroylglutamate
0.01
-
L-homocysteine
-
or less
0.013
-
L-homocysteine
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.014
-
L-homocysteine
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.022
-
L-homocysteine
-
-
0.004
-
S-adenosylmethionine
-
-
0.0022
-
S-methylmethionine
-
-
0.055
-
S-methylmethionine
Phaseolus sp.
-
-
0.016
-
selenohomocysteine
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.417
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.6
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.12
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
at 25C
0.4
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.533
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.283
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.35
-
5-methyltetrahydropteroyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.33
-
L-homocysteine
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
0.417
-
L-homocysteine
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
999
-
-
specific activity after growth in different media
additional information
-
-
42.11 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 11.7fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
additional information
-
-
29.3 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 5.6fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
5-methyltetrahydropteroylglutamate
6.5
-
Q8CWX6, -
0.155 micromol/min/mg, at pH 6.0: 0.152 micromol/min/mg, at pH 7.0: 0.148 micromol/min/mg, at pH 4.6: 0.0096 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg
7.3
-
-
L-methionine
7.9
-
Phaseolus sp.
-
L-methionine
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.6
9
Q8CWX6, -
at pH 4.6: 0.0096 micromol/min/mg, at pH 6.5: 0.155 micromol/min/mg, at pH 9.0: 0.119 micromol/min/mg
6
8.7
-
pH 6.0: about 70% of maximum activity, pH 8.7: about 90% of maximum activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
-
-
calculated from sequence of cDNA
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Escherichia coli K12
-
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Shewanella sp. (strain W3-18-1)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
75000
-
-
gel filtration, sedimentation data
80000
-
-
SDS-PAGE
84000
-
-
equilibrium ultracentrifugation
85000
-
-
gel filtration, recombinant enzyme
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
?
-
x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein
?
Escherichia coli K12
-
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
-
monomer
-
1 * 85000, SDS-PAGE, recombinant enzyme
monomer
Q8CWX6, -
gel filtration
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cocrystallization with L-homocysteine or met and ternary complexes with folate substrates, sitting drop method
-
hanging drop vapour diffusion method
-
hanging-drop vapor diffusion method
Q8CWX6, -
an unusual-barrel structure in which the active site lies between the tops of the two (betaalpha)8 barrels
-
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
Q9X112
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
oxidative stress inactivates the enzyme
-
660332
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DEAE Sepharose column chromatography
-
DEAE column chromatography
-
rapid one-step purification
-
-
Phaseolus sp.
-
HiTrap heparin column chromatography
-
immobilized metal ion affinity chromatography (Ni2+), gel fitration
Q8CWX6, -
Ni2+-chelating chromatography, and S200 gel filtration
-
Zn(II)-NTA column chromatography
Q9X112
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Saccharomyces cerevisiae strain DAY4.1
-
expressed in Escherichia coli strain K-12
-
expressed in Escherichia coli strain MTD23
-
homologous expression and heterologous expression in Saccharomyces cerevisiae
-, Q6GYJ7
expressed in Saccharomyces cerevisiae strain DAY4.1
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3)
Q8CWX6, -
expressed in Escherichia coli BL21(DE3)Star cells
Q9X112
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dramatically induced by GroE depletion
-
the MET1gene is downregulated 2.4fold referred to germinated seeds in response to sucrose
B2VQE0
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C645A
-
the mutation confers resistance to diamide when cells are grown in media lacking methionine, but not when cells are grown in the presence of methionine, cysteine 645 serves to modulate the activity of MetE in vivo in response to disulfide stress
C726S
-
mutant does not contain zinc, no activity, probably due to lack of zinc