Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Populus tomentosa |
Crystallization (Comment) | Organism |
---|---|
modeling of structure. Protein has a typical glutathione S-transferase structure containing a smaller thioredoxin-like N-terminal domain and a larger helical C-terminal domain | Populus tomentosa |
Protein Variants | Comment | Organism |
---|---|---|
D72A | reduction in catalytic efficincy. D72 is a glutathione-site residue | Populus tomentosa |
K8A | severe reduction in catalytic efficincy. K8 is a glutathione-site residue | Populus tomentosa |
S73A | reduction in catalytic efficincy. S73 is a glutathione-site residue | Populus tomentosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
0.13 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
0.23 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
0.48 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
0.7 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
0.97 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
1.71 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
2.28 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
2.47 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
3.01 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
3.26 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
3.7 | 5 | glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Populus tomentosa | 9507 | - |
cytosol | - |
Populus tomentosa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | 40000 | gel filtration | Populus tomentosa |
29100 | - |
1 * 29100, calculated, about 2900, MALDI-TOF | Populus tomentosa |
29700 | - |
1 * 29700, calculated, about 2900, MALDI-TOF | Populus tomentosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | J9WN12 | isoform DHAR2, c.f. EC 2.5.1.18 | - |
Populus tomentosa | J9WNR5 | isoform DHAR3, c.f. EC 2.5.1.18 | - |
Populus tomentosa | J9WQY6 | isoform DHAR1, c.f. EC 2.5.1.18 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + dehydroascorbate | - |
Populus tomentosa | glutathione disulfide + ascorbate | - |
? | |
additional information | no substrates: 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, nitrobutyl chloride, 4-nitrophenyl acetate, 1,2-dichloro-4-nitrobenzene | Populus tomentosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29100, calculated, about 2900, MALDI-TOF | Populus tomentosa |
monomer | 1 * 29700, calculated, about 2900, MALDI-TOF | Populus tomentosa |
Synonyms | Comment | Organism |
---|---|---|
DHAR1 | - |
Populus tomentosa |
DHAR2 | - |
Populus tomentosa |
DHAR3 | - |
Populus tomentosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Populus tomentosa |
45 | - |
- |
Populus tomentosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
66% of maximum activity | Populus tomentosa |
55 | - |
51% of maximum activity | Populus tomentosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
570 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
745 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
3988 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
5737 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
6705 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
13130 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
15960 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
17730 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
21150 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
40850 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
53880 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
99830 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Populus tomentosa |
8 | - |
- |
Populus tomentosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | more than 66% of maximum activity | Populus tomentosa |
6.5 | - |
41% of maximum activity | Populus tomentosa |
8.5 | - |
86% of maximum activity | Populus tomentosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
247 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
588 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
2056 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
5697 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
7708 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
9332 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
16540 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
18760 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
23630 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
26620 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
44050 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
44130 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |