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Literature summary for 1.8.5.1 extracted from

  • Tang, Z.X.; Yang, H.L.
    Functional divergence and catalytic properties of dehydroascorbate reductase family proteins from Populus tomentosa (2013), Mol. Biol. Rep., 40, 5105-5114.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Populus tomentosa

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure. Protein has a typical glutathione S-transferase structure containing a smaller thioredoxin-like N-terminal domain and a larger helical C-terminal domain Populus tomentosa

Protein Variants

Protein Variants Comment Organism
D72A reduction in catalytic efficincy. D72 is a glutathione-site residue Populus tomentosa
K8A severe reduction in catalytic efficincy. K8 is a glutathione-site residue Populus tomentosa
S73A reduction in catalytic efficincy. S73 is a glutathione-site residue Populus tomentosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
0.13
-
dehydroascorbate mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
0.23
-
dehydroascorbate wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
0.48
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
0.7
-
dehydroascorbate mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
0.97
-
dehydroascorbate mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
1.71
-
glutathione mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
2.28
-
glutathione wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
2.47
-
glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa
3.01
-
glutathione mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
3.26
-
glutathione mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
3.7 5 glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Populus tomentosa 9507
-
cytosol
-
Populus tomentosa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000 40000 gel filtration Populus tomentosa
29100
-
1 * 29100, calculated, about 2900, MALDI-TOF Populus tomentosa
29700
-
1 * 29700, calculated, about 2900, MALDI-TOF Populus tomentosa

Organism

Organism UniProt Comment Textmining
Populus tomentosa J9WN12 isoform DHAR2, c.f. EC 2.5.1.18
-
Populus tomentosa J9WNR5 isoform DHAR3, c.f. EC 2.5.1.18
-
Populus tomentosa J9WQY6 isoform DHAR1, c.f. EC 2.5.1.18
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
pH not specified in the publication, temperature not specified in the publication Populus tomentosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + dehydroascorbate
-
Populus tomentosa glutathione disulfide + ascorbate
-
?
additional information no substrates: 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, nitrobutyl chloride, 4-nitrophenyl acetate, 1,2-dichloro-4-nitrobenzene Populus tomentosa ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29100, calculated, about 2900, MALDI-TOF Populus tomentosa
monomer 1 * 29700, calculated, about 2900, MALDI-TOF Populus tomentosa

Synonyms

Synonyms Comment Organism
DHAR1
-
Populus tomentosa
DHAR2
-
Populus tomentosa
DHAR3
-
Populus tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
Populus tomentosa
45
-
-
Populus tomentosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15
-
66% of maximum activity Populus tomentosa
55
-
51% of maximum activity Populus tomentosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
570
-
dehydroascorbate mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
745
-
glutathione mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
3988
-
dehydroascorbate mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
5737
-
dehydroascorbate mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
6705
-
glutathione mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
13130
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
15960
-
glutathione mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
17730
-
dehydroascorbate wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
21150
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
40850
-
glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa
53880
-
glutathione wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
99830
-
glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Populus tomentosa
8
-
-
Populus tomentosa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 more than 66% of maximum activity Populus tomentosa
6.5
-
41% of maximum activity Populus tomentosa
8.5
-
86% of maximum activity Populus tomentosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
247
-
glutathione mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
588
-
dehydroascorbate mutant K8A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
2056
-
glutathione mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
5697
-
dehydroascorbate mutant S73A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
7708
-
dehydroascorbate wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
9332
-
glutathione mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
16540
-
glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa
18760
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
23630
-
glutathione wild-type, pH not specified in the publication, temperature not specified in the publication Populus tomentosa
26620
-
glutathione pH not specified in the publication, temperature not specified in the publication Populus tomentosa
44050
-
dehydroascorbate pH not specified in the publication, temperature not specified in the publication Populus tomentosa
44130
-
dehydroascorbate mutant D72A, pH not specified in the publication, temperature not specified in the publication Populus tomentosa