Cloned (Comment) | Organism |
---|---|
recombinant protein is overexpressed in Escherichia coli | Pinus bungeana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
dehydroascorbate | Vmax: 34.12 micromol/min/mg | Pinus bungeana | |
1.03 | - |
glutathione | Vmax: 136.20 micromol/min/mg | Pinus bungeana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24260 | - |
calculated from cDNA | Pinus bungeana |
29690 | - |
molecular mass of the recombinant protein including a 6xHis tag determined by SDS-PAGE and MALDI-TOF/MS | Pinus bungeana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus bungeana | B2ZHM6 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography | Pinus bungeana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | RT-PCR reveals that the PbDHAR is a constitutive expression gene in Pinus bungeana | Pinus bungeana | - |
needle | - |
Pinus bungeana | - |
phloem | - |
Pinus bungeana | - |
root | - |
Pinus bungeana | - |
seedling | the expression level of PbDHAR mRNA in Pinus bungeana seedlings do not show significant change under high temperature stress | Pinus bungeana | - |
stem | - |
Pinus bungeana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.84 | - |
- |
Pinus bungeana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Pinus bungeana | glutathione disulfide + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | native PAGE, MALDI-TOF/MS | Pinus bungeana |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Pinus bungeana |
PbDHAR | - |
Pinus bungeana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the recombinant PbDHAR is a thermostable enzyme, retaining 77% of its initial activity at 55°C | Pinus bungeana |
70 | - |
at 70°C PbDHAR retains 46% of its initial activity | Pinus bungeana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.15 | - |
dehydroascorbate | - |
Pinus bungeana | |
40.53 | - |
glutathione | - |
Pinus bungeana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39.34 | - |
glutathione | - |
Pinus bungeana | |
126.9 | - |
dehydroascorbate | - |
Pinus bungeana |