Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.5.1 extracted from

  • Yang, H.L.; Zhao, Y.R.; Wang, C.L.; Yang, Z.L.; Zeng, Q.Y.; Lu, H.
    Molecular characterization of a dehydroascorbate reductase from Pinus bungeana (2009), J. Integr. Plant Biol., 51, 993-1001.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein is overexpressed in Escherichia coli Pinus bungeana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
dehydroascorbate Vmax: 34.12 micromol/min/mg Pinus bungeana
1.03
-
glutathione Vmax: 136.20 micromol/min/mg Pinus bungeana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24260
-
calculated from cDNA Pinus bungeana
29690
-
molecular mass of the recombinant protein including a 6xHis tag determined by SDS-PAGE and MALDI-TOF/MS Pinus bungeana

Organism

Organism UniProt Comment Textmining
Pinus bungeana B2ZHM6
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity chromatography Pinus bungeana

Source Tissue

Source Tissue Comment Organism Textmining
additional information RT-PCR reveals that the PbDHAR is a constitutive expression gene in Pinus bungeana Pinus bungeana
-
needle
-
Pinus bungeana
-
phloem
-
Pinus bungeana
-
root
-
Pinus bungeana
-
seedling the expression level of PbDHAR mRNA in Pinus bungeana seedlings do not show significant change under high temperature stress Pinus bungeana
-
stem
-
Pinus bungeana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.84
-
-
Pinus bungeana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
Pinus bungeana glutathione disulfide + ascorbate
-
?

Subunits

Subunits Comment Organism
monomer native PAGE, MALDI-TOF/MS Pinus bungeana

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
Pinus bungeana
PbDHAR
-
Pinus bungeana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
the recombinant PbDHAR is a thermostable enzyme, retaining 77% of its initial activity at 55°C Pinus bungeana
70
-
at 70°C PbDHAR retains 46% of its initial activity Pinus bungeana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.15
-
dehydroascorbate
-
Pinus bungeana
40.53
-
glutathione
-
Pinus bungeana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
39.34
-
glutathione
-
Pinus bungeana
126.9
-
dehydroascorbate
-
Pinus bungeana