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Literature summary for 1.8.5.1 extracted from

  • Jiang, Y.C.; Huang, C.Y.; Wen, L.; Lin, C.T.
    Dehydroascorbate reductase cDNA from sweet potato (Ipomoea batatas [L.] Lam): expression, enzyme properties, and kinetic studies (2008), J. Agric. Food Chem., 56, 3623-3627.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ipomoea batatas

General Stability

General Stability Organism
resistant to digestion by trypsin and chymotrypsin even at a high enzyme/substrate (w/w) ratio of 1:10 Ipomoea batatas
slight decrease in activity with increasing concentration of imidazole to 0.8 M Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
dehydroascorbate 25°C Ipomoea batatas
2.38
-
glutathione 25°C Ipomoea batatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 25000, SDS-PAGE Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas D2CGD4 (L.) Lam
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Ipomoea batatas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
-
Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
Ipomoea batatas glutathione disulfide + ascorbate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
Ipomoea batatas
DHAR
-
Ipomoea batatas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 10.1 min Ipomoea batatas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100.6
-
glutathione 25°C Ipomoea batatas
368.2
-
dehydroascorbate 25°C Ipomoea batatas

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 stable Ipomoea batatas