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Literature summary for 1.8.5.1 extracted from

  • Kim, Y.R.; Kang, S.O.
    Purification and characterization of dehydroascorbate reductase from Pleurotus ostreatus (1998), J. Microbiol., 36, 164-170.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 34% loss of activity Pleurotus ostreatus
Cd2+ 5 mM CdCl2, complete loss of activity Pleurotus ostreatus
Cu2+ 0.1 mM CuSO4, 43% loss of activity Pleurotus ostreatus
Fe2+ 0.1 mM FeSO4, 34% loss of activity Pleurotus ostreatus
HgCl2 5 mM HgCl2, 94% loss of activity Pleurotus ostreatus
imidazole 1 mM, 14% loss of activity Pleurotus ostreatus
iodoacetic acid 1 mM, 26% loss of activity Pleurotus ostreatus
Mn2+ 5 mM MnSO4, 82% loss of activity Pleurotus ostreatus
NEM 1 mM, 23% loss of activity Pleurotus ostreatus
PCMB 1 mM, 32% loss of activity Pleurotus ostreatus
Sodium azide 1 mM, 32% loss of activity Pleurotus ostreatus
Zn2+ 5 mM ZnSO4, 50% loss of activity Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
GSH
-
Pleurotus ostreatus
2.5
-
dehydroascorbate
-
Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Pleurotus ostreatus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
gel filtration Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.3
-
-
Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSH + dehydroascorbate specific for glutathione as hydrogen donor Pleurotus ostreatus GSSG + ascorbate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 86000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38 45
-
Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 6.2, 1 h, stable below Pleurotus ostreatus
65
-
complete loss of activity above Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pleurotus ostreatus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 25% of maximal activity, pH 8.5: about 60% of maximal activity Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
highest stability at, unstable under acidic and highly alkaline conditions Pleurotus ostreatus

Cofactor

Cofactor Comment Organism Structure
flavin enzyme shows an unusual flavin peak, enzyme might form a flavin adduct Pleurotus ostreatus