Cloned (Comment) | Organism |
---|---|
overexpression into Escherichia coli | Psychrobacter sp. ANT206 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 0.25 mM, 62.7% loss of activity | Psychrobacter sp. ANT206 | |
CaCl2 | 0.25 mM, 9.1% loss of activity | Psychrobacter sp. ANT206 | |
CdCl2 | 0.25 mM, 80.9% loss of activity | Psychrobacter sp. ANT206 | |
CoCl2 | 0.25 mM, 72.7% loss of activity | Psychrobacter sp. ANT206 | |
CrCl2 | 0.25 mM, 52.7% loss of activity | Psychrobacter sp. ANT206 | |
CuCl2 | 0.25 mM, 79.7% loss of activity | Psychrobacter sp. ANT206 | |
EDTA | 0.25 mM, 71.8% loss of activity | Psychrobacter sp. ANT206 | |
FeCl2 | 0.25 mM, 55.5% loss of activity | Psychrobacter sp. ANT206 | |
HgCl2 | 0.25 mM, 89.1% loss of activity | Psychrobacter sp. ANT206 | |
KCl | 0.25 mM, 9.7% loss of activity | Psychrobacter sp. ANT206 | |
Pb(NO3)2 | 0.25 mM, complete loss of activity | Psychrobacter sp. ANT206 | |
ZnCl2 | 0.25 mM, 66.4% loss of activity | Psychrobacter sp. ANT206 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01695 | - |
NADPH | pH 7.5, 25°C | Psychrobacter sp. ANT206 | |
0.06896 | - |
glutathione disulfide | pH 7.5, 25°C | Psychrobacter sp. ANT206 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.25, 1.1fold activation | Psychrobacter sp. ANT206 | |
NaCl | most active in 1.0 M NaCl and 62.5% of its full activity remains in 3.0 M NaCl, demonstrating its high salt tolerance | Psychrobacter sp. ANT206 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53500 | - |
SDS-PAGE | Psychrobacter sp. ANT206 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | Psychrobacter sp. ANT206 | - |
2 glutathione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter sp. ANT206 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Psychrobacter sp. ANT206 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
168.8 | - |
pH 7.5, 25°C | Psychrobacter sp. ANT206 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Psychrobacter sp. ANT206 | 2 glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53500, SDS-PAGE | Psychrobacter sp. ANT206 |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Psychrobacter sp. ANT206 |
psgr | - |
Psychrobacter sp. ANT206 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Psychrobacter sp. ANT206 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 45 | 5°C: about 50% of maximal activity, 45°C: about 55% of maximal activity | Psychrobacter sp. ANT206 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
60 min, about 15% loss of activity | Psychrobacter sp. ANT206 |
30 | - |
60 min, about 20% loss of activity | Psychrobacter sp. ANT206 |
35 | - |
60 min, about 40% loss of activity | Psychrobacter sp. ANT206 |
40 | - |
60 min, about 45% loss of activity | Psychrobacter sp. ANT206 |
45 | - |
60 min, about 55% loss of activity | Psychrobacter sp. ANT206 |
50 | - |
60 min, about 60% loss of activity | Psychrobacter sp. ANT206 |
55 | - |
60 min, complete loss of activity | Psychrobacter sp. ANT206 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95.78 | - |
NADPH | pH 7.5, 25°C | Psychrobacter sp. ANT206 | |
95.78 | - |
glutathione disulfide | pH 7.5, 25°C | Psychrobacter sp. ANT206 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Psychrobacter sp. ANT206 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 50% of maximal activity, pH 8.5: about 70% of maximal activity | Psychrobacter sp. ANT206 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
30 min, about 60% loss of activity | Psychrobacter sp. ANT206 |
5 | - |
30 min, about 50% loss of activity | Psychrobacter sp. ANT206 |
5.5 | - |
30 min, about 40% loss of activity | Psychrobacter sp. ANT206 |
6 | - |
30 min, about 35% loss of activity | Psychrobacter sp. ANT206 |
6.5 | - |
30 min, about 30% loss of activity | Psychrobacter sp. ANT206 |
7 | - |
30 min, about 15% loss of activity | Psychrobacter sp. ANT206 |
7.5 | - |
30 min, about less than 5% loss of activity | Psychrobacter sp. ANT206 |
8 | - |
30 min, about 15% loss of activity | Psychrobacter sp. ANT206 |
8.5 | - |
30 min, about 30% loss of activity | Psychrobacter sp. ANT206 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme possesses the flavin adenine dinucleotide binding motif | Psychrobacter sp. ANT206 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme provides protection against peroxide (H2O2)-induced oxidative stress in recombinant cells | Psychrobacter sp. ANT206 |