Information on EC 1.8.1.7 - glutathione-disulfide reductase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.8.1.7
-
RECOMMENDED NAME
GeneOntology No.
glutathione-disulfide reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glutathione metabolism
-
-
Glutathione metabolism
-
-
glutathione-glutaredoxin redox reactions
-
-
glutathione-peroxide redox reactions
-
-
SYSTEMATIC NAME
IUBMB Comments
glutathione:NADP+ oxidoreductase
A dimeric flavoprotein (FAD); activity is dependent on a redox-active disulfide in each of the active centres.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-48-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Achromobacter starkeyi
-
-
-
Manually annotated by BRENDA team
iron-grown, AP19-3
-
-
Manually annotated by BRENDA team
iron-grown, AP19-3
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Amphiprora kufferathii
-
-
-
Manually annotated by BRENDA team
strain 7119, filamentous cyanobacterium
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cyanobacterium
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
toad
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Colwellia sp.
strain MH2
-
-
Manually annotated by BRENDA team
Colwellia sp. MH2
strain MH2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
yeast, IFO 0895
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 011
-
-
Manually annotated by BRENDA team
strain S33
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
L. cv. Ehimehadaka no. 1
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
cestode
-
-
Manually annotated by BRENDA team
no activity in Drosophila melanogaster
-
-
-
Manually annotated by BRENDA team
no activity in Trypanosoma sp.
-
-
-
Manually annotated by BRENDA team
sainfoin
-
-
Manually annotated by BRENDA team
strain ATCC 24466 and 24467
SwissProt
Manually annotated by BRENDA team
Pibocella sp.
strain MH3
-
-
Manually annotated by BRENDA team
Pibocella sp. MH3
strain MH3
-
-
Manually annotated by BRENDA team
isozymes from the cold-hardened strains GR-1H and GR-2H and noncold-hardened strains GR1NH and GR-2NH; red spruce
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Chinese brake fern
-
-
Manually annotated by BRENDA team
Wistar
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Sulfitobacter sp.
strain MH1
-
-
Manually annotated by BRENDA team
Sulfitobacter sp. MH1
strain MH1
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-
Manually annotated by BRENDA team
parasite isolated from mice, enzyme is a multifunctional thioredoxin-glutathione reductase TGR
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-
Manually annotated by BRENDA team
Trachemys scripta elegans
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-
Manually annotated by BRENDA team
durum wheat
-
-
Manually annotated by BRENDA team
parasitic protozoa
-
-
Manually annotated by BRENDA team
parasitic protozoa
-
-
Manually annotated by BRENDA team
cultivars EPACE-1 and 1183
-
-
Manually annotated by BRENDA team
Xanthomonas campestris pv. phaseoli
TrEMBL
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-dihydroxy-9,10-anthraquinone + NADPH
?
show the reaction diagram
1,4-naphthoquinone + NADPH
?
show the reaction diagram
1,4-naphthoquinone + NADPH + H+
?
show the reaction diagram
1,8-dihydroxy-9,10-anthraquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
2,3-dimethylquinoxaline-5,8-dione + NADPH + H+
?
show the reaction diagram
2,5-bis(aziridin-1-yl)-3,6-dimethylcyclohexa-2,5-diene-1,4-dione + NADPH
?
show the reaction diagram
-
-
-
-
?
2,5-bis(aziridin-1-yl)-3-(hydroxymethyl)-6-methylcyclohexa-2,5-diene-1,4-dione + NADPH
?
show the reaction diagram
-
-
-
-
?
2,5-bis(aziridin-1-yl)cyclohexa-2,5-diene-1,4-dione + NADPH
?
show the reaction diagram
-
-
-
-
?
2,5-bis(ethylamino)-3,6-di(aziridinyl)-1,4-benzoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
2,6-dimethyl-1,4-benzoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
2-hydroxy-1,4-naphthoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
2-hydroxy-3-methyl-1,4-naphthoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
2-methyl-1,4-naphthoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
show the reaction diagram
-
-
-
-
?
5,8-dihydroxy-1,4-naphthoquinone + NADPH
?
show the reaction diagram
5-hydroxy-1,4-naphthoquinone + NADPH
?
show the reaction diagram
6,7-dimethylquinoline-5,8-dione + NADPH + H+
?
show the reaction diagram
6-methylquinoline-5,8-dione + NADPH + H+
?
show the reaction diagram
7-methylquinoline-5,8-dione + NADPH + H+
?
show the reaction diagram
9,10-phenanthrene quinone + NADPH
?
show the reaction diagram
-
-
-
-
?
ajoene + NADPH
4,5,9-trithiadodeca-1,6,11-triene + NADP+
show the reaction diagram
ajoene + NADPH
4,8,9,13-tetrathiahexadeca-1,6,1,15-tetraene + NADP+
show the reaction diagram
AuCl4- + NADPH + H+
?
show the reaction diagram
-
formation of gold nanoparticles at the active site of glutathione reductase that are tightly bound through the catalytic cysteines. The nanoparticles can be removed from the glutathione reductase active site with thiol reagents such as 2-mercaptoethanol
-
-
?
bis-N-chloro-gamma-L-glutamyl derivative of GSSG + NADPH + H+
? + glutathione + NADP+
show the reaction diagram
-
-
-
-
?
diallyl trisulfide + NADPH
?
show the reaction diagram
-
-
-
-
?
diethyl [2,5-bis(aziridin-1-yl)-3,6-dioxocyclohexa-1,4-diene-1,4-diyl]biscarbamate + NADPH
?
show the reaction diagram
-
-
-
-
?
glutathione disulfide + NADH + H+
2 glutathione + NAD+
show the reaction diagram
-
-
-
?
glutathione disulfide + NADH + H+
glutathione + NAD+
show the reaction diagram
glutathione disulfide + NADPH
glutathione + NADP+
show the reaction diagram
glutathione disulfide + NADPH + H+
glutathione + NADP+
show the reaction diagram
GSSG + NADPH
glutathione + NADP+
show the reaction diagram
GSSG + NADPH + H+
glutathione + NADP+
show the reaction diagram
GSSG + NADPH + H+
GSH + NADP+
show the reaction diagram
menadione + NADPH + H+
?
show the reaction diagram
peroxynitrite + NADPH
nitrite + H2O + NADP+
show the reaction diagram
quinoline-5,8-dione + NADPH + H+
?
show the reaction diagram
quinoxaline-5,8-dione + NADPH + H+
?
show the reaction diagram
S-nitrosoglutathione + NADPH + H+
? + NADP+
show the reaction diagram
-
-
-
-
?
tetramethyl-1,4-benzoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
trimethyl-1,4-benzoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
trimethyl-aziridinyl-1,4-benzoquinone + NADPH
?
show the reaction diagram
-
-
-
-
?
trypanothione + NADPH
reduced trypanothione + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutathione disulfide + NADPH
glutathione + NADP+
show the reaction diagram
glutathione disulfide + NADPH + H+
glutathione + NADP+
show the reaction diagram
GSSG + NADPH
glutathione + NADP+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
deamino-NADPH
-
69% of the activity with NADPH
ferricyanide
-
-
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
0.05 mM, 2fold stimulation
diphosphate
-
activates
KBr
-
below 0.1 M
KCl
-
IC50: 0.51 M
KH2PO4
-
below 0.1 M
Mn2+
-
activates
Na+
-
activates
NaBr
-
activates
NaCl
-
activates
NaF
-
below 0.1 M
NH4+
-
activates
NH4Cl
-
below 0.1 M
phosphate
selenium
-
regulates the enzyme expression pattern, enzyme contains a catalytic selenocysteine
SO42-
-
stimulates
Sodium acetate
-
activates
Sodium citrate
-
activates
Sodium phosphate
-
activates
Zn2+
-
glutathione reductase is non-competitively inhibited up to 2 mM and activated above this concentration
additional information
-
optimum ionic strength of enzyme is 50 mM Tris–HCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R(S),2R(S),3S(R),4S(R))-2,3-dihydroxycyclo-hexane-1,4-diyl dinitrate
-
non-competitive inhibition
(1R(S),2R(S),4R(S),5R(S))-2,5-dihydroxycyclo-hexane-1,4-diyl dinitrate
-
non-competitive inhibition
(1S(R),2S(R),5R(S),6R(S))-5-bromo-9-oxabicyclo[4.2.1] nonan-2-yl nitrate
-
non-competitive inhibition
(1S(R),3S(R),4S(R),6S(R))-4,6-dihydroxycyclo-hexane-1,3-diyl dinitrate
-
non-competitive inhibition
(2R(S),7R(S))-7-hydroxybicyclo[2.2.1]heptan-2-yl nitrate
-
non-competitive inhibition
(2S(R),7R(S))-7-hydroxybicyclo[2.2.1] heptan-2-yl nitrate
-
non-competitive inhibition
(9R(S))-hydroxy-1,2,3,4-tetrahydro-1,4-methano-naphthalen-2R(S)-yl nitrate
-
non-competitive inhibition
1,2-bis[methylsulfonyl]-1-[2-[chloroethyl]-2-(methylamino)carbonyl]hydrazine
-
0.05 mM, 28% inhibition
1,2-Cyclohexanedione
-
-
1,3-Bis(2-chloroethyl)-1-nitrosourea
-
significant inhibition of recombinant isoform GR1 is observed at high concentrations of 1 mM and above
1,3-bis-(2-chloroethyl) 1-nitrosourea
1,3-Bis-(2-chloroethyl)-1-nitrosourea
1,3-bis[2-chloroethyl]-2-nitrosourea
1,4-dihydroxy-9,10-anthraquinone
1,4-Naphthoquinone
1,8-dihydroxy-9,10-anthraquinone
1-(2-Chloroethyl)-3-(2-hydroxyethyl)-1-nitrosourea
-
-
1-chloro-2,4-dinitrobenzene
-
reversible
1-Fluoro-2,4-dinitrobenzene
-
reversible
1-methyl-4-(2-methyl-1,3-dioxo-2,3,5a,9a-tetrahydropyrido[3,4-b]quinoxalin-5(1H)-yl)pyridinium
-
poor inhibitor
11-(3-methyl-1,4-dioxo-1,4-dihydronaphthalen-2-yl)undecanoic acid
2,3-Butanedione
-
-
2,3-dimethylquinoxaline-5,8-dione
2,4,6-Trinitrobenzenesulfonate
2,4-dihydroxybenzylamine
-
specific inhibitor, complete inhibition at 0.001 mM
-
2,5-bis(aziridin-1-yl)-3,6-bis[(2-hydroxyethyl)amino]cyclohexa-2,5-diene-1,4-dione
-
-
2,5-bis(aziridin-1-yl)-3,6-dimethylcyclohexa-2,5-diene-1,4-dione
-
-
2,5-bis(aziridin-1-yl)-3-(hydroxymethyl)-6-methylcyclohexa-2,5-diene-1,4-dione
2,5-bis(aziridin-1-yl)cyclohexa-2,5-diene-1,4-dione
-
-
2,5-bis(ethylamino)-3,6-di(aziridinyl)-1,4-benzoquinone
2,6-dimethyl-1,4-benzoquinone
2-acetylamino-3-[4-(2-acetylamino-2-carboxy-ethylsulfanylthio carbonylamino)phenylthiocarbamoylsulfanyl]propionic acid
-
irreversible and selective glutathione reductase inhibitor, almost complete inhibition at 0.1 mM for 1 h, thereafter, the enzyme activity starts to return and reaches 63% of the control at the end of 8 h
2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]propionic acid
-
irreversible glutathione reductase inhibitor
2-Chloroethylisocyanate
-
-
2-Hydroxy-1,4-naphthoquinone