Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Colwellia psychrerythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
0.0109 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95240 | - |
gel filtration | Colwellia psychrerythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins | Colwellia psychrerythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Colwellia psychrerythraea | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE, 2 * 48700, MALDI-TOF | Colwellia psychrerythraea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
3 h, 75% resiudal activity | Colwellia psychrerythraea |
50 | - |
half-life 40 min | Colwellia psychrerythraea |
55 | - |
half-life 8 min | Colwellia psychrerythraea |
60 | - |
half-life below 2 min | Colwellia psychrerythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
217 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
700 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins | Colwellia psychrerythraea | |
NADPH | - |
Colwellia psychrerythraea |
General Information | Comment | Organism |
---|---|---|
physiological function | gene is able to complement an Escherichia coli glutathione reductase deletion strain in oxidative stress growth assays | Colwellia psychrerythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.7 | - |
NADPH | 4°C, pH not specified in the publication | Colwellia psychrerythraea | |
63.3 | - |
NADPH | 25°C, pH not specified in the publication | Colwellia psychrerythraea |