Protein Variants | Comment | Organism |
---|---|---|
C44S | 0.003% of the activity of wild-type enzyme with NAD+ and dihydrolipoamide. Enzyme is capable to catalyze reactions with NADH as electron donor and ferricyanide, thio-NAD+, 2,6-dichlorophenol indophenol and O2 as electron acceptor. The fluorescence of FAD in oxidized wild-type enzyme is markedly temperature dependent, while the fluorescence of FAD in mutants C44S and C49S is independent of temperature | Escherichia coli |
C49S | 0.012% of the activity of wild-type enzyme with NAD+ and dihydrolipoamide. Enzyme is capable to catalyze reactions with NADH as electron donor and ferricyanide, thio-NAD+, 2,6-dichlorophenol indophenol and O2 as electron acceptor. The fluorescence of FAD in oxidized wild-type enzyme is markedly temperature dependent, while the fluorescence of FAD in mutants C44S and C49S is independent of temperature | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | activity with wild-type enzyme and mutant enzymes C44S and C49S | Escherichia coli | 2 ferrocyanide + NAD+ + H+ | - |
? | |
dihydrolipoamide + NAD+ | mutant enzymes C44S and C49S show minute activity | Escherichia coli | lipoamide + NADH | - |
? | |
O2 + NADH | activity with wild-type enzyme and mutant enzymes C44S and C49S | Escherichia coli | ? | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADH | activity with wild-type enzyme and mutant enzymes C44S and C49S | Escherichia coli | ? + NAD+ | - |
? | |
thio-NAD+ + NADH | activity with wild-type enzyme and mutant enzymes C44S and C49S | Escherichia coli | thio-NADH + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the fluorescence of FAD in oxidized wild-type enzyme is markedly temperature dependent, while the fluorescence of FAD in mutants C44S and C49S is independent of temperature | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |