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Literature summary for 1.3.1.53 extracted from

  • Saller, E.; Laune, H.; Oppenberg, H.R.S.; Cook, A.M.
    Purification and some properties of (1R,2S)-1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2 (1995), FEMS Microbiol. Lett., 130, 97-102.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
NAD+
-
Comamonas testosteroni
0.09
-
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate
-
Comamonas testosteroni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Comamonas testosteroni
60000
-
-
Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ Comamonas testosteroni the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate Protocatechuic acid + NADH + CO2
-
?
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ Comamonas testosteroni T-2 the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate Protocatechuic acid + NADH + CO2
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
T-2
-
Comamonas testosteroni T-2
-
T-2
-

Purification (Commentary)

Purification (Comment) Organism
-
Comamonas testosteroni

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:p-toluate-grown cell
-
Comamonas testosteroni
-
culture condition:terephthalate-grown cell
-
Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ the hydride ion is transferred to the si face of NAD+, i.e. B-specific Comamonas testosteroni Protocatechuic acid + NADH + CO2
-
?
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate Comamonas testosteroni Protocatechuic acid + NADH + CO2
-
?
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ the hydride ion is transferred to the si face of NAD+, i.e. B-specific Comamonas testosteroni T-2 Protocatechuic acid + NADH + CO2
-
?
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate Comamonas testosteroni T-2 Protocatechuic acid + NADH + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Comamonas testosteroni