Information on EC 1.3.1.53 - (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase

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The expected taxonomic range for this enzyme is: Comamonas testosteroni

EC NUMBER
COMMENTARY
1.3.1.53
-
RECOMMENDED NAME
GeneOntology No.
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ = 3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
-
-
-
-
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ = 3,4-dihydroxybenzoate + CO2 + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
4-toluenecarboxylate degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
Polycyclic aromatic hydrocarbon degradation
-
SYSTEMATIC NAME
IUBMB Comments
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate:NAD+ oxidoreductase
Requires FeII. Involved in the terephthalate degradation pathway in bacteria [2].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
-
-
-
-
(1R,2S)-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
-
-
-
-
Dehydrogenase, 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
162032-77-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain YZW-D
-
-
Manually annotated by BRENDA team
Comamonas testosteroni T-2
T-2
-
-
Manually annotated by BRENDA team
Comamonas testosteroni YZW-D
strain YZW-D
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
-
the hydride ion is transferred to the si face of NAD+, i.e. B-specific
-
-
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
-
the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
-
-
-
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
Comamonas testosteroni T-2
-
the hydride ion is transferred to the si face of NAD+, i.e. B-specific
-
-
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
Comamonas testosteroni T-2
-
the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
-
-
-
cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,4-dicarboxylate + NAD+
3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni YZW-D
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni T-2
-
the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
-
-
-
cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,4-dicarboxylate + NAD+
3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni YZW-D
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Fe2+
-
activates
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.09
-
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Comamonas testosteroni T-2
-
-
-
Manually annotated by BRENDA team
Comamonas testosteroni T-2
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 39000, SDS-PAGE
dimer
Comamonas testosteroni T-2
-
2 * 39000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE