Information on EC 1.3.1.53 - (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase

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The expected taxonomic range for this enzyme is: Comamonas testosteroni

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
1.3.1.53
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SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase
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SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ = 3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
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-
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(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ = 3,4-dihydroxybenzoate + CO2 + NADH + H+
show the reaction diagram
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SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-toluenecarboxylate degradation
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Polycyclic aromatic hydrocarbon degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate:NAD+ oxidoreductase
Requires FeII. Involved in the terephthalate degradation pathway in bacteria [2].
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
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(1R,2S)-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
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Dehydrogenase, 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate
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-
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SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
162032-77-1
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SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain YZW-D
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Manually annotated by BRENDA team
Comamonas testosteroni T-2
T-2
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Manually annotated by BRENDA team
Comamonas testosteroni YZW-D
strain YZW-D
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Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
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the hydride ion is transferred to the si face of NAD+, i.e. B-specific, the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
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(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
Comamonas testosteroni T-2
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the hydride ion is transferred to the si face of NAD+, i.e. B-specific, the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
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cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,4-dicarboxylate + NAD+
3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni YZW-D
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-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
Protocatechuic acid + NADH + CO2
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni T-2
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the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate
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cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,4-dicarboxylate + NAD+
3,4-dihydroxybenzoate + CO2 + NADH
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni YZW-D
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-
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
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activates
SplaateInhibitors, Organism, Commentary, Literature,Filename
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.09
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate
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0.043
NAD+
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SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Comamonas testosteroni T-2
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Manually annotated by BRENDA team
Comamonas testosteroni T-2
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Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60000
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12307
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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2 * 39000, SDS-PAGE
dimer
Comamonas testosteroni T-2
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2 * 39000, SDS-PAGE
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SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature