Literature summary for 1.2.3.1 extracted from

Mahro, M.; Bras, N.F.; Cerqueira, N.M.; Teutloff, C.; Coelho, C.; Romao, M.J.; Leimkuehler, S.
Identification of crucial amino acids in mouse aldehyde oxidase 3 that determine substrate specificity (2013), PLoS ONE, 8, e82285.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
F1014I	amino acid exchange in the active site	Mus musculus
F1014L	amino acid exchange in the active site, 10fold increase in molybdenim content	Mus musculus
F1014V	amino acid exchange in the active site	Mus musculus
F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L	all residues in the first coordination sphere around the substrate are exchanged to their counterparts in bovine xanthine oxidoreductase. Mutant shows activity towards benzaldehyde, phthalazine and hypoxanthine	Mus musculus
F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L	all residues in the first coordination sphere around the substrate except K889 are exchanged to their counterparts in bovine xanthine oxidoreductase, mutant is devoid of activity towards most substrates tested, while allopurinnol is oxidized at a low rate	Mus musculus
additional information	exchange of several residues in the active site to the ones found in other Aox homologues in mouse or to residues present in bovine xanthine oxidoreductase. Conversion of Aox3 to an xanthine oxidoreductase is achieved exchanging eight residues in the active site. Exchange of the iron-sulfur clusters FeSI, FeSII and both FeSI/FeSII by the corresponding domains of isoform Aox1 results in a decrease in catalytic activity with all substrates tested and both electron acceptor dichlorophenol indophenol and O2	Mus musculus
P1015A	amino acid exchange in the active site	Mus musculus
P1015G	amino acid exchange in the active site	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0014	-	Phthalazine	wild-type, pH 8.0,37°C	Mus musculus
0.0022	-	benzaldehyde	mutant P1015A, pH 8.0,37°C	Mus musculus
0.0025	-	benzaldehyde	wild-type, pH 8.0,37°C	Mus musculus
0.0026	-	Phthalazine	mutant P1015A, pH 8.0,37°C	Mus musculus
0.0033	-	benzaldehyde	mutant P1015G, pH 8.0,37°C	Mus musculus
0.009	-	Phthalazine	mutant F1014I, pH 8.0,37°C	Mus musculus
0.0103	-	Phthalazine	mutant P1015G, pH 8.0,37°C	Mus musculus
0.0125	-	Phthalazine	mutant F1014V, pH 8.0,37°C	Mus musculus
0.0215	-	benzaldehyde	mutant F1014V, pH 8.0,37°C	Mus musculus
0.0227	-	benzaldehyde	mutant F1014I, pH 8.0,37°C	Mus musculus
0.032	-	phenanthridine	wild-type, pH 8.0,37°C	Mus musculus
0.047	-	phenanthridine	mutant P1015G, pH 8.0,37°C	Mus musculus
0.073	-	phenanthridine	mutant F1014I, pH 8.0,37°C	Mus musculus
0.087	-	N1-methyl-nicotineamide	mutant P1015A, pH 8.0,37°C	Mus musculus
0.089	-	N1-methyl-nicotineamide	mutant P1015G, pH 8.0,37°C	Mus musculus
0.099	-	phenanthridine	mutant P1015A, pH 8.0,37°C	Mus musculus
0.128	-	N1-methyl-nicotineamide	wild-type, pH 8.0,37°C	Mus musculus
0.133	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
0.385	-	phenanthridine	mutant F1014V, pH 8.0,37°C	Mus musculus
0.426	-	allopurinol	mutant P1015G, pH 8.0,37°C	Mus musculus
0.482	-	N1-methyl-nicotineamide	mutant F1014V, pH 8.0,37°C	Mus musculus
0.527	-	allopurinol	mutant P1015A, pH 8.0,37°C	Mus musculus
0.733	-	Phthalazine	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
1.595	-	allopurinol	wild-type, pH 8.0,37°C	Mus musculus
2.21	-	benzaldehyde	mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
2.93	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
3.86	-	allopurinol	mutant F1014V, pH 8.0,37°C	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Molybdenum	-	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	G3X982	isoform Aox3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
allopurinol + H2O + 2,6-dichlorophenolindophenol	-	Mus musculus	? + reduced 2,6-dichlorophenolindophenol	-	?
benzaldehyde + H2O + 2,6-dichlorophenolindophenol	-	Mus musculus	benzoate + reduced 2,6-dichlorophenolindophenol	-	?
N1-methyl-nicotineamide + H2O + 2,6-dichlorophenolindophenol	-	Mus musculus	? + reduced 2,6-dichlorophenolindophenol	-	?
phenanthridine + H2O + O2	-	Mus musculus	6-phenanthridone + H2O2	-	?
phthalazine + H2O + 2,6-dichlorophenol indophenol	-	Mus musculus	? + reduced 2,6-dichlorophenolindophenol	-	?

Synonyms

Synonyms	Comment	Organism
AOX3	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.18	-	phenanthridine	mutant F1014I, pH 8.0,37°C	Mus musculus
0.27	-	allopurinol	mutant F1014V, pH 8.0,37°C	Mus musculus
0.31	-	benzaldehyde	mutant F1014I, pH 8.0,37°C	Mus musculus
0.38	-	N1-methyl-nicotineamide	mutant F1014V, pH 8.0,37°C	Mus musculus
0.4	-	N1-methyl-nicotineamide	wild-type, pH 8.0,37°C	Mus musculus
0.62	-	allopurinol	mutant P1015G, pH 8.0,37°C	Mus musculus
0.77	-	allopurinol	mutant P1015A, pH 8.0,37°C	Mus musculus
0.97	-	Phthalazine	mutant F1014I, pH 8.0,37°C	Mus musculus
1.08	-	N1-methyl-nicotineamide	mutant P1015A, pH 8.0,37°C	Mus musculus
1.1	-	Phthalazine	wild-type, pH 8.0,37°C	Mus musculus
1.13	-	benzaldehyde	wild-type, pH 8.0,37°C	Mus musculus
1.22	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
1.22	-	allopurinol	wild-type, pH 8.0,37°C	Mus musculus
1.27	-	benzaldehyde	mutant P1015A, pH 8.0,37°C	Mus musculus
1.42	-	phenanthridine	mutant F1014V, pH 8.0,37°C	Mus musculus
1.42	-	phenanthridine	wild-type, pH 8.0,37°C	Mus musculus
1.55	-	benzaldehyde	mutant P1015G, pH 8.0,37°C	Mus musculus
1.58	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
1.92	-	N1-methyl-nicotineamide	mutant P1015G, pH 8.0,37°C	Mus musculus
2.13	-	benzaldehyde	mutant F1014V, pH 8.0,37°C	Mus musculus
2.98	-	Phthalazine	mutant P1015A, pH 8.0,37°C	Mus musculus
3.9	-	benzaldehyde	mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
4.63	-	Phthalazine	mutant F1014V, pH 8.0,37°C	Mus musculus
5.78	-	Phthalazine	mutant P1015G, pH 8.0,37°C	Mus musculus
8.73	-	Phthalazine	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
8.83	-	phenanthridine	mutant P1015G, pH 8.0,37°C	Mus musculus
9.68	-	phenanthridine	mutant P1015A, pH 8.0,37°C	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
[2Fe-2S]-center	contains two [2Fe2S] clusters	Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.00008	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
0.0001	-	allopurinol	mutant F1014V, pH 8.0,37°C	Mus musculus
0.0007	-	allopurinol	wild-type, pH 8.0,37°C	Mus musculus
0.0008	-	N1-methyl-nicotineamide	mutant F1014V, pH 8.0,37°C	Mus musculus
0.0015	-	allopurinol	mutant P1015A, pH 8.0,37°C	Mus musculus
0.0015	-	allopurinol	mutant P1015G, pH 8.0,37°C	Mus musculus
0.0018	-	benzaldehyde	mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
0.0027	-	phenanthridine	mutant F1014I, pH 8.0,37°C	Mus musculus
0.0031	-	N1-methyl-nicotineamide	wild-type, pH 8.0,37°C	Mus musculus
0.0037	-	phenanthridine	mutant F1014V, pH 8.0,37°C	Mus musculus
0.0119	-	allopurinol	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
0.0119	-	Phthalazine	mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C	Mus musculus
0.0124	-	N1-methyl-nicotineamide	mutant P1015A, pH 8.0,37°C	Mus musculus
0.013	-	benzaldehyde	mutant F1014I, pH 8.0,37°C	Mus musculus
0.0216	-	N1-methyl-nicotineamide	mutant P1015G, pH 8.0,37°C	Mus musculus
0.044	-	phenanthridine	wild-type, pH 8.0,37°C	Mus musculus
0.098	-	benzaldehyde	mutant F1014V, pH 8.0,37°C	Mus musculus
0.098	-	phenanthridine	mutant P1015A, pH 8.0,37°C	Mus musculus
0.11	-	Phthalazine	mutant F1014I, pH 8.0,37°C	Mus musculus
0.189	-	phenanthridine	mutant P1015G, pH 8.0,37°C	Mus musculus
0.37	-	Phthalazine	mutant F1014V, pH 8.0,37°C	Mus musculus
0.45	-	benzaldehyde	wild-type, pH 8.0,37°C	Mus musculus
0.47	-	benzaldehyde	mutant P1015G, pH 8.0,37°C	Mus musculus
0.56	-	Phthalazine	mutant P1015G, pH 8.0,37°C	Mus musculus
0.58	-	benzaldehyde	mutant P1015A, pH 8.0,37°C	Mus musculus
0.79	-	Phthalazine	wild-type, pH 8.0,37°C	Mus musculus
1.148	-	Phthalazine	mutant P1015A, pH 8.0,37°C	Mus musculus