Application | Comment | Organism |
---|---|---|
synthesis | a multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol | Paraburkholderia xenovorans |
Cloned (Comment) | Organism |
---|---|
gene bphC, recombinant expression in Escherichia coli | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Paraburkholderia xenovorans | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Paraburkholderia xenovorans | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | low activity | Paraburkholderia xenovorans | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Paraburkholderia xenovorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
additional information | multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol, substrate specificity and molecular docking, product identification by HPLC-MS, overview | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxy-biphenyl 1,2-dioxygenase | - |
Paraburkholderia xenovorans |
BphCLA-4 | - |
Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paraburkholderia xenovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | over 60% at pH 6.0 and 9.0, and less than 50% catalytic efficiency at pH 5.0 and 10.0, strain BphCLA-4 | Paraburkholderia xenovorans |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology modeling, overview | Paraburkholderia xenovorans |